استفاده از زادمایه های باکتری سودوموناس فلورسنس محرک رشد گیاه در افزایش رشد و جذب عناصر غذایی گیاه گندم

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه تهران

2 استاد گروه علوم و مهندسی خاک دانشگاه تهران

3 استاد گروه شیمی دانشگاه آزاد رشت

چکیده

هزینه بالای تولید کودهای شیمیایی و ایجاد آلودگی‌های زیست محیطی ناشی از مصرف این کودها، استفاده از منابع دیگر به ویژه کودهای زیستی را ضروری می‌سازد. هدف از این پژوهش بررسی تاثیر کود زیستی باکتری حل کننده فسفات‌ و محرک رشد گیاه سودوموناس فلوئورسنس بر پایه حامل‌های مختلف آلی و معدنی شامل ورمی‌کمپوست، پرلیت و خاک فسفات و چهار فرمولاسیون از این مواد، بر روی شاخص‌های رشد گیاه گندم بوده است. آزمایش در قالب طرح بلوک‌های کاملا تصادفی و با چهار تکرار، 7 تیمار زادمایه، 2 تیمار کودی سوپرفسفات ساده و تریپل و شاهد صورت گرفت. نتایج نشان داد اگرچه در مورد صفات سبزینگی، ارتفاع، فسفر اندام هوایی، وزن خشک اندام هوایی و ریشه، تیمار سوپرفسفات تریپل و ساده بهتر بوده ولی تفاوت معنی-داری با زادمایه‌های حاوی ورمی‌کمپوست نداشتند (P>0.05). در مورد صفات روی و آهن اندام هوایی زادمایه ورمی‌کمپوست بیشترین مقدار را دارا بوده و در مورد صفت روی اندام هوایی تفاوت معنی‌داری با تیمارهای کودی و شاهد داشت (P

کلیدواژه‌ها


عنوان مقاله [English]

The use of plant growth promotion bacteria inoculants Pseudomonas fluorescens in increasing growth and nutrient uptake in wheat

چکیده [English]

High cost of fertilizers manufacture and environmental pollution due to their application make it necessary to use other sources especially biofertilizers. The purpose of this study was to determine capability of bacteria Pseudomonas fluorescence survival as one of phosphate solubilizing and plant growth promoting bacteria on different organic and inorganic carriers including vermicompost, perlite, rock phosphate, and four formulations of them and also, their effect on growth indices in wheat. The experiment was performed through complete randomized block design with four replicates, seven inoculants and two fertilizers (simple and triple superphosphate) treatments. Based on the results although triple super phosphate treatment was the best regarding to chlorophyll, plant height, phosphorus content in the shoot, and shoot and root dry weight attributes but did not show significant difference with inoculants containing vermicompost (P>0.05). About the Iron and zinc in the shoot, vermicompost inoculant was observed higher than other treatments, and about zinc in the shoot this difference was significant with fertilizer and control (p

کلیدواژه‌ها [English]

  • Biofertilizer
  • Carrier
  • Superphosphate triple
  • Vermicompost
References

Adesemoye A. O., and Kloepper J. W. 2009. Plant-microbes interactions in enhanced fertilizer-use efficiency. Applied Microbiology and Biotechnology, 85:1-12.

Ahmad F., Ahmad I., and Khan M.S. 2006. Screening of free living rhizosphere bacteria for their multiple plant growth promoting activities. Microbiological Research, 163: 173-181.

Albareda M., Rodriguez-Navarro D. N., Camacho M., and Temprano, F. J. 2008. Alternatives to peat as a carrier for rhizobia inoculants: solid and liquid formulations. Soil Biology and Biochemistry, 2008: 40: 2771-2779.

Alexander D.B., and Zuberer D.A. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biology and Fertility of Soils, 12: 39-45.

Amico E.D., Cavalca L., and Andreoni V. 2005. Analysis of rhizobacterial communitiesin perennial Graminaceae from polluted water meadow soil, and screeningof metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiol Ecology, 52: 153-162.

Ardakani S.S., Hedari A., Tayebi L., and Mohammadi M. 2010 Promotion of cotton seedlings growth characteristics by development and use of new Bioformulations. International Journal of Botany, 6: 95-100.

Arora N. K., Tiwari S., and Singh R. 2014. Comparative study of different carriers inoculated with nodule forming and free living plant growth promoting bacteria suitable for sustainable agriculture. Journal of Plant Pathology and Microbiology, 5(2):1-3.

Azarmi F., Mozafari V., Abbaszadeh P., and Hamidpour M. 2014. Isolation and evaluation of plant growth promoting indices of Pseudomonas fluorescens isolated from Pistachio rhizosphere. Journal of Soil Biology, 2(2):173-186. (In Persian)

Bashan Y. 1998. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances, 16 (4): 729-770.

Bashan Y., Bustillos J.J., Leyva L.A., Hernandez J.P., and Bacilio M. 2006. Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by azospirillum brasilence. Biology and Fertility of Soil, 42: 279-285.

Besharati H., Saleh rastin N., Malakouti M., and Alizade A. 2004. The investigation on viability potential of Thiobacillus on several kinds of carriers. Journal of soil and water sciences, 18(2):170-181. (In Persian)

Carter M.R., and Gregorich E.G. 2008. Soil Sampling and Methods of Analysis (2nd Ed.), CRC Press. Boca Raton, Florida, 1204p.

Fernandez-Luqueno F., Reyes-Varela V., Martinez-Suarez C., Salomon-Hernandez G., Yanez-Meneses J., Ceballos-Ramerez J.M., and Dendooven L. 2010. Effect of different nitrogen sources on plant characteristics and yield of common bean (Phaseolus vulgaris L.). Bioresource Technology, 101: 396–403.

Gandi A., and Sivakumar K. 2010. Impact of vermicompost carrier based bioinoculants on growth, yield and quality of rice (oryza sativa). An International Quarterly Journal of Environmental Science 4(1): 83-88.

Ghevariya K.K., and Desai P.B. 2014. Rhizobacteria of sugarcane: In vitro screening for their plant growth promoting potentials. Research Journal of Recent Sciences, 3: 52-58.

Glick B. R., Penrose D., and Wendo M. 2001. Bacterial promotion of plant growth. Biotechnology Advanced, 19:135-138.

Glick B.R. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol Research, 169:30–9.

Hamidi A., Asgharzadeh A., Choukan R., Dehghan Shoar M., Ghalavand A., and Malakouti M.J. 2010. Effects of pgpr application on dry matter partitioning and some growth characteristics of maize (zea mays L.) hybrids under greenhouse conditions. Iranian Journal of Soil Research, 24 (1):55-67. (In Persian)

Hellal F.A., Zewainy R.M., Khalil A.A., and Ragab A.A.M. 2014. Effect of organic and bio-fertilizer management practices on nutrient availability and uptake by faba bean- maize sequence. American-Eurasian Journal of Sustainable Agriculture, 8 (5): 35-42.

Jain R., Saxena J., and Sharma V. 2012. Effect of phosphate solubilizing fungi Aspergillus awamori S29 on mungbean (Vigna radiata cv. RMG 492) growth. Folia Microbiologica, 57 (6): 533-541.

Javanmard A., Nazari B., Jalilian A., and Dashti S. 2015. Response of wheat to vermicompost and chemical fertilizers residual in soil. Sustainable Agriculture and Production Science, 25(4):87-103. (In Persian)

Jeon J. S., Lee S.S., Kim H.Y., Ahn T. S. and Song H. G. 2003. Plant growth promoting in soil by some inoculated microorganism. Journal of Microbioogyl, 271-276.

Joshi R., Vig A.P., and Singh, J. 2013. Vermicompost as soil supplement to enhance growth, yield and quality of Triticum aestivum L.: a field study. International Journal of Recycling of Organic Waste in Agriculture, 2(16):1-7.

Kaur G., and Reddy M.S. 2015. Effects of phosphate solublizing bacteria, rock phosphate and chemical fertilizer on maize-wheate cropping cycle and economics. Pedosphere, 25(3):428-437.

Larsen J., Cornejo P., and Miguel Barea J. 2009. International between the arbuscular mycorrhizal fungus Glomus intraradices and the plant growth promoting rhizobacteria Paenibacillus polymyxa and P. macerans in the mycorrhizosphere of cucumsi sativas. Soil Biology and Biochemistry, 41: 286-292.

Lindsay W. L., and Norvell W. A. 1978. Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of American Journal, 42: 421-428.

Lujan A.M., Gomez P., and Buckling A. 2015. Siderophore cooperation of the bacterium Pseudomonas fluorescens in soil. Biology Letters, 11: 20140934.

Magnucka E.G., and Pietr S. J. 2015. Various effects of fluorescent bacteria of the genus Pseudomonas containing ACC deaminase on wheat seedling growth. Microbiological Research, 181: 112–119.

Moradi M., Siadat S.A., Khavazi K., Naseri R., Maleki A., and Mirzae A. 2011. Effect of application of biofertilizers and phosphorus fertilizers on qualitative and quantitative traits of spring wheat (Triticum aestivum L.). Journal of Crop and Weed Ecophysiology, 5(18): 51-66.

Nada W.M., Rensburg L.V., Claassens S., and Blumenstein O. 2011. Effect of vermicompost on soil and plant properties of coal spoil in the lusatian region (eastern Germany). Communications in Soil Science and Plant Analysis, 42:1945–1957.

Packialakshmi N., and Aliya Riswana T. 2014. Screening and production of phosphate solubilising bacterial inoculants using different carrier. Research Journal of Pharmaceutical, Biological and Chemical Sciences, (2): 1762.

Patten C.L., and Glick B. R. 2002. Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and Enviromental Microbiology, 68:3795-3801.

Reetha S., Bhuvaneswari G., Thamizhiniyan P., and Ravi Mycin T. 2014. Isolation of indole acetic acid (IAA) producing rhizobacteria of Pseudomonas fluorescens and Bacillus subtilis and enhance growth of Onion (Allim cepa.L). International Journal of Current Microbiology and Applied Sciences, 3(2): 568-574.

Ryan J., Estefan G., and Rashid R. 2001. Soil and Plant Analysis Laboratory Manual. 2nd (Ed.). Available from ICARDA, Aleppo, Syria, 172 p.

Sara thambalm C., Thangaraju M., Paulraj C., and Gomathy, M. 2010. Assessing the zinc solubilization ability of Gluconocetobacter diazotrophicus in maize rhizosphere using labeled Zn compounds. Indian Journal of Microbiology, 50(1): 103-109.

Schoebitz M., Ceballos C., and Ciampi L. 2013. Effect of immobilized phosphate solubilizing bacteria on wheat growth and phosphate uptake.Journal of Soil Science and Plant Nutrition, 13 (1):1-10.

Sheikhi J., Ronaghi A., Karimian N., Zarei M., and Yasrebi J. 2015. Influence of arbuscular mycorrhizal fungi and vermicompost on biological yield, concentration and uptake of micronutrients in wheat cultivars ‘Bahar and Shiraz’. Journal of Soil Biology, 3(1):45-57. (In Persian)

Sinha R.K., Dalsukh V., Krunal C., and Sunita A. 2010. Embarking on a second green revolution for sustainable agriculture by vermiculture biotechnology using earthworms: Reviving the dreams of Sir Charles Darwin. Journal of Agricultural Biotechnology and Sustainable, 2 (7): 113-128.

Tahmasbi F., Lakzian A., Khavazi K., and Pakdin Parizi, A. 2014. Isolation, identification and evaluation of sidrophore production in Pseudomonas bacteria and its effect on hydroponically grown corn. Journal of Soil Biology, 3 (1):75-87. (In Persian)

Takahashi S., and Anwar M. R. 2007. Wheat grain yield phosphorus uptake and soil phosphorus fraction after 23 y of annual fertilizer application to an Andosol. Field Crops Research, 101: 160-171.

Theunissen J., Ndakidemi P. A., and Laubscher C. P. 2010. Potential of vermicompost produced from plant waste on the growth and nutrient status in vegetable production. International Journal of Physical Science, 5(13):1964-1973.

Wilhem J. M., Johnson F., Karlen L., and David T. 2007. Corn stover sustain soil organic carbon further constrains biomass supply. Agronomy Journal, 99:1665-1667.

Young L.S., Hameed A., Peng S.Y., Shan Y.H., and Wu S.P. 2013. Endophytic establishment of the soil isolate Burkholderia sp. CC-Al74 enhances growth and P-utilization rate in maize (Zea mays L.). Applied Soil Ecology, 66: 40-47.