تاثیر ورمی‌کمپوست هرس درختان سیب بر بهبود برخی خواص بیولوژیک خاک آلوده به سرب

نوع مقاله: مقاله پژوهشی

نویسندگان

1 فارغ‌التحصیل کارشناسی ارشد گروه علوم خاک، دانشکده کشاورزی دانشگاه ارومیه

2 دانشیار گروه علوم خاک، دانشکده کشاورزی دانشگاه ارومیه

چکیده

سرب (Pb) از پرخطرترین عوامل آلودگی زیست­محیطی می­باشد که بر سلامت انسان و حیوانات تأثیر سوء دارد. حضور سرب در خاک باعث کاهش فعالیت­های بیولوژیکی خاک نیز می­شود. هدف از این پژوهش، بررسی تاثیر ورمی­کمپوست در کاهش اثر آلودگی سربی بر فعالیت ریزجانداران خاک بود. بدین منظور آزمایش گلخانه­ای به صورت فاکتوریل در قالب طرح پایه کاملا تصادفی شامل فلز سرب (Pb) در چهار سطح (غلظت­های 0، 250، 500 و 1000 میلی­گرم بر کیلوگرم) و ورمی­کمپوست (C) ضایعات هرس درختان سیب در سه سطح (0، 20 و 40 تن در هکتار) و در سه تکرار انجام شد. یک نمونه خاک با نمک نیترات سرب به­طوریکنواخت برای ایجاد غلظت­های مختلف، آلوده شد. بعد از سپری شدن دوره خواباندن (90 روز)، برخی شاخص­های بیولوژیک خاک اندازه­گیری گردید. نتایج نشان داد که با افزایش غلظت سرب در خاک تنفس پایه میکروبی و برانگیخته و کربن زیست توده میکروبی به شدت کاهش یافتند. ولی با افزایش سطوح ورمی­کمپوست، این شاخص­های بیولوژیک بهبود یافتند. کاربرد 20 و 40 تن در هکتار ورمی­کمپوست تنفس پایه میکروبی را بطور میانگین به­ترتیب بیش از 95/1 و 33/2 برابر نسبت به شاهد افزایش داد. کربن زیست­توده میکروبی بطور میانگین در تیمار 40 تن در هکتار ورمی­کمپوست، 18/3 برابر نسبت به تیمار شاهد بیش­تر بود. مقدار qCO2 در تیمار 20 کیلوگرم در هکتار ورمی­کمپوست نسبت به تیمار شاهد بیش از 6/2 برابر افزایش نشان داد. بنابراین می­توان نتیجه گرفت که کاربرد ورمی­کمپوست باعث رفع تاثیر سوء سرب بر فعالیت ریزجانداران خاک می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Apple Pruning Vermicompost on Improving some Biological Properties of a Pb-Contaminated Soil

نویسندگان [English]

  • Parinaz Khodaei 1
  • MirHassan Rasouli-Sadaghiani 2
  • Habib Khodaverdiloo 2
چکیده [English]

Lead (Pb) is one of dangerous environmental contaminated factors which threaten the health of plants, animals and humans. Lead in the soil led to reduce the biological activities of soil. The aim of this study was to evaluate the effect of vermicompost (C) of apple pruning wastes on microorganism’s activity of a Pb-contaminated soil. For this purpose an experiment was carried out in greenhouse condition as a factorial experiment based on a randomized completely design with two factors including Pb concentration in four levels (0, 250, 500 and 1000 mg Pb kg-1 soil) and vermicompost in three levels (0, 20 and 40 t ha-1). A soil sample was selected and spiked uniformly with different concentrations of Pb. These samples were incubated for 90 days, and then some biological experiments were measured. Results showed that by increasing the Pb concentration in soil, BR, SIR and MBC were reduced intensively, but these indices were improved by adding the vermicompost. Using 20 and 40 t ha-1 vermicompost increased BR up to 1.95 and 2.33 times higher than control treatment, respectively. MBC at 40 t ha-1 vermicompost application treatment on average was 3.18 times higher than control treatment. The amount of qCO2 increased up to 2.6 times in C20 (20 t ha-1 vermicompost) treatment compared to control. Therefore it could be concluded that application of vermicompost ameliorate the toxic impacts of Pb on microorganisms activity.  

کلیدواژه‌ها [English]

  • lead (Pb)
  • Metabolic Quotient
  • Microbial biomass carbon (MBC)
  • Microbial respiration
  • Vermicompost
References

Alef K., and Nannipieri P. 1995. Methods in Applied Soil Microbiology and Biochemistry.  Academic Press, London, 453p.

Alkorta I., Aizpurua A., Riga P., Albizu I., Amezaga I., and Garbisu C. 2003. Soil enzyme activities as biological indicators of soil health. Reviews on Environmental Health, 18: 65–73.

Alloway B.J. 1990.  Heavy Metals in Soils:  Lead.  Blackie and Son Ltd, Publishers, Bishop Briggs, Glasgow, London, pp.  177-196.

Anderson J.P. 1982. Soil Respiration. In: A.L. and Mille R.H. (Eds.), Methods of Soil Analysis Part 2, Chemical and Microbiological Properties. American Society of Agronomy, Madison, WI, pp. 831-871.

Anderson T.H., and Domsch K.H. 1993. The metabolic quotient from CO2 (qCO2) as a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils. Soil Biology and Biochemistry, 25: 393–395.

Baath E. 1989. Effects of heavy metals in soil on microbial processes and populations. Water, Air, and Soil Pollution, 47: 335-379.

Belyaeva O.N., Haynes R.J. and Birukova O.A. 2005. Barley yield and soil microbial and enzyme activities as affected by contamination of two soils with lead, zinc or copper. Biology and Fertility of Soils, 41: 85-94.

Cariny T. 1995. The Reuse of Contaminated Land. John Wiley and Sons Ltd. Publisher, 219p.

Carter M.R., and Gregorich, E.G. 2008. Soil Sampling and Methods of Analysis, 2nd ed. CRC Press, Boca Raton, FL, 1204p.

Cherif H., Ayari F., Ouzari H., Marzorati M., Brusetti L., Jedidi N., Hassen A. and Daffonchio D. 2009. Effects of municipal solid waste compost, farmyard manure and chemical fertilizers on wheat growth, soil composition and soil bacterial characteristics under Tunisian arid climate. European Journal of Soil Biology, 45: 138-145.

Epelde L., Hernandez-allica J., Becerril J.M., and Garbisu C. 2006. Assessment of the efficiency of a metal phytoremediation process with biological indicators of soil health. In: Difpolmine Conference, 12-14 December, Le Corum, Montpel- lier, France.

Gai N., Yang Y., Li T., Yao J., Wang F., and Chen H. 2011. Effect of lead contamination on soil microbial activity measured by micro-calorimetry. Chinese Journal of Chemistry, 29: 1541-1547.

Giller K.E., Witter F., and McGrath S.P. 1998. Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils, a review. Soil Biology and Biochemistry, 30: 1389-1414.

Hattori H. 1992.  Influence of heavy metals on soil microbial activities. Soil Science and Plant Nutrition, 38: 93-100.

Haynes R.J., Fraser P.M., Piercy J.E., and Tregurtha R.J. 2003. Casts of Aporrectodea caliginosa (Savigny) and Lumbricus rubellus (Hoffmeister) differ in microbial activity, nutrient availability and aggregate stability. Pedobiologia, 47: 882–887.

Khan A.G. 2005.  Role of soil microbes in the rhizosphere of plants growing on trace element contaminated soils in phytoremediation. Journal of Trace Elements in Medicine and Biology, 18: 355-364.

Kennedy A.C., and Smith K.L. 1995. Soil microbial diversity and the sustainability of agricultural soils. Plant and Soil, 170: 75-86.

Knasmuller S., Gottmann E., Steinkellner H., Fomin A., Pickl C., and Paschke A. 1998. Detection of genotoxic effects of heavy metal contaminated soils with plant bioassays. Mutation Research, 420: 37-48.

Jenkinson D.S., and Ladd J.N. 1981. Microbial biomass in soil measurement and turnover, In: Paul E.A., and Ladd J.N. (Eds). Soil Biochemistry, Marcel Dekker, Inc. pp. 415-471.

Liao M., and Xie X.M.  2007. Effect of heavy metals on substrate utilization pattern, biomass, and activity of microbial communities in a reclaim wasteland of red soil area. Ecotoxicology and Environmental Safety, 66: 17-223.

Landi L., Renella G., Moreno J.L., Falchini L., and Nannipieri P. 2000. Influence of cadmium on  the  metabolic  quotient,  l-D-glutamic  acid  respiration  ratio  and  enzyme  activity:  microbial biomass ratio under laboratory conditions. Biology and Fertility of Soils, 32: 8-16.

McLaughlin M.J., Hamon R.E., McLaren R.G., Speir T.W., and Rogers S.L. 2000. A bioavailability-based rationale for controlling metal and metalloid contamination of agricultural land in Australia and New Zealand. Australian Journal of Soil Research, 38: 1037-1086.

Moreno J.L., Bastida F., Ros M., Hernandez T., and Garcia C. 2009. Soil organic carbon buffers heavy metal contamination on semiarid soils: effects of different metal threshold levels on soil microbial activity. European Journal of Soil Biology, 45: 220-228.

Nannipieri P. 1994. The potential use of soil enzymes as indicators of productivity, sustainability and pollution. In: Pankhurst C.E., Doube B.M., Gupta V.V.S.R., and Grace P.R. (Eds). Soil Biota: Management in Sustainable Farming Systems. CSIRO Publications, Melbourne, Australia, pp. 238-244.

Nelson R.E., and Sommers L.E. 1982. Total carbon, organic carbon and organic matter. In: Page, A.L., Miller R.H., and Keeney D.R. (Eds.), Methods of Soil Analysis. Part 2. Soil Science Society of America, Madison, WI. pp. 539-579.

Nwachukwu O.I., and Pulford I.D. 2011. Microbial respiration as an indication of metal toxicity in contaminated organic materials and soil. Journal of Hazardous Materials, 185: 1140-1147.

Obbard P. 2001. Ecotoxicological assessment of heavy metals in sewage sludge amended soils. Applied Geochemistry, 16: 1405-1411.

Papa S., Bartoli Pellegrino G., and Fioretto A.A. 2010. Microbial activities and trace element contents in an urban. Plant, Soil and Environment, 165:193-203.

Raskin I., and Ensley B.D. 2000. Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment. John Wiley and Sons, Inc. New York 304p.

Vig K., Megharaj M., Sethunathan N., and Naidu R. 2003. Bioavailability and toxicity of lead to microorganisms and their activities in soil: a review. Advances in Environmental Research, 8: 121-135.

Verma R. K., Yadav D.V., Singh C.P., Suman A., and Gaur A. 2010.  Effect of heavy metals on soil respiration during decomposition of sugarcane (Saccharum officinarum L.) trash in different soils. Plant, Soil and Environment, 56: 76-81.

Wang Y.P., Shi J.Y., Lin Q., Chen X., and Chen Y.X. 2007. Heavy metal availability and impact on activity of soil microorganisms along a Cu/Zn contamination gradient. Journal of Environmental Sciences, 19: 848-853.

Zhang Y., Zhang H.W., Su, Z.C., and Zhang C.G. 2008. Soil microbial characteristics under long-term heavy metal stress: a case study in Zhangshi wastewater irrigation area, Shenyang. Pedosphere, 18: 1-10.