Effects of Humic Acid, Vermicompost and Zinc Foliar Spray on Some Quantitative and Qualitative Characteristics of Pomegranate (Khazar Bardaskan cv.)

Document Type : Original Article

Authors

1 PhD student, Department of Soil Science, College of Agriculture, Ferdowsi University of Mashhad, Iran

2 Professor, Department of Soil Science, College of Agriculture, Ferdowsi University of Mashhad, Iran

3 Assistant Professor of Soil and Water Research, Department Agriculture and Natural Resources Research and Education Center of Khorasan Razavi, Agricultural Research, Education & Extension organization, Iran

4 Associate professor, Department of Soil Science, Agricultural College, Ferdowsi University of Mashhad

Abstract

Abstract
Considering the high cultivation area of pomegranate in Iran, it is necessary to pay attention to this plant nutrition in order to increase quantitative and qualitative of pomegranate fruit. Nutrition management is one of the most important factors for determining the yield and quality of fruit that there are a few studies to reach the optimal management of nutrition in pomegranate. Therefore, the present study was conducted to investigate the effect of different levels of organic component and chemical fertilizers on quantity and quality characteristics of Punica granatum fruit cv. KhazarBardaskanas a factorial design based on randomized complete blocks design with three replications. Treatments include vermicompost in three levels of 0, 12.5 and 18.75 (V1, V2, V3) kg tree-1 and humic acid in two levels of 0 and 5 g tree-1 (H1, H2) as the soil application and zinc chelate in the two levels of 0 and 0.005 (Zn1, Zn2) as foliar spray. The measured factors were yield, length and diameter of fruit and some qualitative characteristics of fruit juice such as total phenol, antioxidant activity, titratable acidity (TA), total soluble solids (TSS). Also, the concentration of nitrogen, phosphorus, potassium and zinc were determined in leaves. The results of present study showed that the effects of treatments were significant for more measured parameters. The maximum amount of yield 29.09 kg tree-1, antioxidant activity (39.79 %) and total phenol (863.12 mg GAE 100g-1) were observed in V2H2Zn2 treatment of 12.5 kg tree-1 vermicompost, 5 g tree-1 humic acid and 0.005 foliar sprayof zinc. Also, the level of nitrogen, potassium and zinc in the leaves were obtained from the above mentioned treatment (V2H2Zn2), which compared with the control treatment can be recommended as the superior treatment.

Keywords


References
Ahmadi K., Ebadzadeh H.R., Hatami F., Hosseinpour R., and Abdshah H. 2018. Statistic information of agriculture of 2016 year (Volume 3). Horticulture. Ministry of Agriculture of Deputy of Planning and Economic Center of Information and Communication Technology. (In Persian)
Alipour H., and Hosseinipour S.J. 2006. Identification and Elimination of Nutrient Deficiencies in Pistachios (2nd Ed.). Pistachio Research Institute of the Country, Rafsanjan, Iran, 53p.
Alloway BJ. 2008. Zinc in Soils and Crop Nutrition (2nd Ed.). International Zinc Association (IZA) and International Fertilizer Industry Association (IFA). Brussels, Belgium and Paris, France, 139p.
Amiri M.E., and Fallahi E. 2009. Impact of animal manure on soil chemistry, mineral nutrients, yield, and fruit quality in Golden Delicious apple. Journal of Plant Nutrition, 32: 610-617.
Anari Anaraki B., Ghasemnezhad M., and Meighani H. 2016. Effect of Foliar and Soil Application of Humic Acid on Quantity and Quality of Pomegranate Fruit cv. 'Malas-E- Saveh'. Knowledge of Agriculture and Sustainable Production, 26(3): 143-153. (In Persian)
Ashiono GB., Wasike W., Ouma JP., Gatwiku SW., and Gachuki PN. 2005. Residual effects of farmyard manure on stover and grain yield of cold tolerant dual purpose sorghum in the dry high land of Kenya. Agronomy, 4: 300-303.
Baladi E., Toselli M., and Marangoni B. 2010. Nutrient partitioning in potted peach (Prunus persica L.) trees supplied with mineral and organic fertilizers. Journal of Plant Nutrition, 33: 2050-2061.
Brand-Williams W., Cuvelier M.E., and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Science Technology, 28: 25–30.
Bremener J.M., and Mulvaney C.S. 1982. Nitrogen-area. In: Page A.L., Miller R.H., and Keeney D.R. (Ed.), Methods of Soil Analysis, Part 2, Chemical and Micro Biological Properties. Agronomy Monograph NO. 9, American Society of Agronomy, Madison, WI.
Cerdán M., Sánchez-Sánchez A., Juárez M., Sánchez-Andreu J.J., Jordá J.D., and Bermúdez D. 2007 Partial replacement of Fe (o,o-EDDHA) by humic substances for Fe nutrition and fruit quality of citrus. Journal of Plant Nutrition and Soil Science, 170: 474–478.
Chapman H.D., and Pratt P.F. 1961. Methods of Analysis for Soils, Plants and Water. University of California, Berkelery, CA, USA, 309p.
Day P.R. 1955. Particle fractionation and particle-size analysis. In: Black, C.A. (Ed.), Method of Soil Analysis, Part 1, Agronomy Monograph NO. 9, American Society of Agronomy, Madison, WI.
Derzhi M.T., Ghalond A., and Rejali F. 2009. The effect of biological fertilizers on the adsorption of K, P, N, and grain yield in fennel (Feniculum Vulgare Mill). Iranian Medicinal and Aromatic Plants Research, 25(1): 1-19. (In Persian)
Deh Dashti Zadeh B., Aria H., Azizi M., and Davari Nejad Gh.H. 2009. The effect of various levels of vermicompost and mineral element of phosphorus on growth and development and absorption of some nutrients in tomato transplant. Iranian Journal of Horticulture, 40(3): 49-58. (In Persian)
Edwards C.A., and Burrows I. 1988. The potential of earthworm composts as plant growth media. In: Edwards C.A. and Neuhauser E.F (Ed.), Earthworms in Waste and Environmental Management, SPB Academic Publ, Co, The Hague, The Netherlands, pp. 211-219.
Fathy MA., Gabr MA., and El Shall SA. 2010. Effect of humic acid treatments on 'Canino' apricot growth, yield and fruit quality. New York Science Journal, 3: 109-115.
Gholami H., Saharkhiz M.J., Raouf Fard F., Ghan A., and Nadaf F. 2018. Humic acid and vermicompost increased bioactive components, antioxidant activity and herb yield of Chicory (Cichorium intybus L.). Biocatalysis and Agricultural Biotechnology, 1-33.
Giasuddin ABM., Kanel S., and Choi H. 2007. Adsorption of humic acid onto nanoscale zerovalent iron and its effect on arsenic removal. Journal Environment Science Technology, 41(6): 2022-2027.
Gil MI., Tomas-Barberan FA., Hess-Pierce B., Holcroft DM., and Kader AA. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48: 4581–4589.
Golshadi A., Ansari R., Asgari S., Sarafzadegan N., and Bashtam M. 2002. Knowledge, belief and practice of herbal medicine in people of Isfahan. Joturnal Medical Plants, 2: 21-28.
Hallmann E. 2012. The influence of organic and conventional cultivation systems on the nutritional value and content of bioactive compounds in selected tomato types. Journal of the Science of Food and Agriculture, 92(14): 2840-2848.
Hasani M., Zamani Z., Savaghebi G., and Fatahi R. 2012. Effects of zinc and manganese as foliar spray on pomegranate yield, fruit quality and leaf minerals. Journal of Soil Science and Plant Nutrition, 12: 471-480.
Hmid I., Elothmani D., Hanine H., Ooukabli A., and mehinagic E. 2013. Comparative study of phenolic compounds and their antioxidant attributes eighteen pomegranate (Punica granatum L.) cultivars grown in Morocco. Arabian Jornal of Chemistery.
Khattab M., Shaban A., El-Shrief HA., and El-Deen-Mohamed A. 2012. Effect of humic acid and amino acids on Pomegranate trees under deficit irrigation. I: growth, flowering and fruiting. Journal of Horticultural Science and Ornamental Plants, 4: 253-259.
Khoshghalb H., Arzani K., Malakouti M.J., and Barzgar M. 2013. Effect of Ca, Zn and B spray application on preharvest fruit drop, sugar and nutrient contents and on some quantitative and qualitative fruit characterestics in some Asian pear (Pyrus serotina Rehd.) cultivars. Iranian Journal of Horticultural Science, 44(2): 149-159. (In Persian)
Kulkarni A.P., and Aradhya S.M. 2005. Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93: 319–324.
Leonel S., and Tecchio M.A. 2009. Cattle manure fertilization increases fig yield. Science Agriculture, 66 (6): 806-811.
Lester G.E., Jifon J.L., and Makus D.J. 2010. Impact of potassium nutrition on postharvest fruit quality: melon (Cucumis melo L.) Case study. Plant and Soil, 335: 117-131.
Mc Lean E.O. 1982. Soil PH and lime requirement. In: Page et al. (Ed.), Methods of Soil Analysis, part 2, Chemical and Micro Biological Properties. Agronomy, Soil Science Society of America, Madison, Wisconsin, USA, pp. 192-224.
Magkos F., Arvaniti F., and Zampelas A. 2003. Organic food: nutritious food or food for thought? A review of the evidence. International Journal of Food Sciences and Nutrition, 54(5): 357–371.
Mahmoud A.R., and Hafez M.M. 2010. Inceasing productivity of potato plants (solanum tuberosum L.) by using potassium fertilizer and humic acid application. International Journal Academic Research, 2:83-88.
Marschner H. 1995. Mineral nutrition of higher plants. (2nd Ed.). Academic Press, San Diego.
Mirdehghan S.H., and Rahemi M. 2007. Seasonal changes of mineral nutrients and phenolics in pomegranate (Punica granatum L.) fruit. Scientia Horticulturae, 111: 120–127.
Mohammadi Z., Rusta H.R., Taj Abadipour A., and Hokam Abadi H. 2013. Effect of nitrogen, organic fertilizer, potassium and iron on crop, fruit quality, and nutrient element concentration in Pistachio noodle fungi on almond rocks. Iranian Journal of Horticulture, 27(2): 117-129. (In Persian)
Nardi S., Pizzeghello D., Muscolo A., and Vianello A. 2002. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, 34: 1527–1536.
Natesan R., Kandasamy S., Thiyageshwari S., and Boopathy PM. 2006. Influence of lignite humic acid on the micronutrient availability and yield of blackgram in an Alfisol. The 18th WCSS, Philadelphiat, Pennsylvania, USA.
Olyaie Torshiz A., Goldansaz S.H., Motesharezadeh B., Asgari Sarcheshmeh M.A., and Zarei A. 2017. Effect of organic and biological fertilizers on pomegranate trees: yield, cracking, sunburning and infestation to pomegranate fruit moth Ectomyelois ceratoniae (Lepidoptera: Pyralidae). Journal of Crop Protection, 6(3): 327-340.
Olsen S.R., and Sommers L.E. 1982. Phosphorous. In: Page, A.L. (Ed.), Methods of Soil Analysis, Chemical and Micro Biological Properties, Part 2, (2nd Ed.). Agronomy Mongraph No. 9, Soil Science Society of America and American Society of Agronomy, Madison, WI.
Page A.L., Miller R.H., and Keeney D.R. 1982. Methods of Soil Analysis, Chemical and Biological Properties, Part 2, (2nd Ed.), Soil Science Society of America and American Society of Agronomy, Madison.
Pansu M., and Gautheyrou J. 2006. Handbook of Soil Analysis: Mineralogical, Organic and Inorganic Methods. Springer-Verlag, Berlin.
Parvin M., Ziaeyan A., Dastfal M., and Ghorbani M.S. 2017. Combined effects of mycorrhizal inoculation, organic fertilizer and potassium application on the yield and chemical composition of pomegranate (Punica granatum L.) leaves. Applied Soil Research, 5(1): 92-103. (In Persian)
Peymani Foroshani A., Pourjavand N., Haghighi M., and Khajeh Ali J. 2017. Effect of vermicompost solids and aqueous extracts on growth characteristics of tomato and White Plate (Trialeurodes vaporariorum).Journal of Greenhouse Crop Science and Technology, 7(26): 35-45. (In Persian)
Poozeshi R., Zabihi H.R., Ramezani Moghaddam M.R., Rajabzadeh M., and Mokhtari A. 2011. Effect of zinc, oxidative and acetic acid spraying on yield, yield components and elemental composition in grapevine Peakani variety. Journal of Horticultural Science, 25(3): 351-360.(In Persian)
Ramezanian A., Rahemi M., and Vazifehshenas MR. 2009. Effects of foliar application of calcium chloride and urea on quantitative and qualitative characteristics of pomegranate fruits. Scientia Horticulturae, 121: 171–175.
Razavi Nasab A., Shirani H., Tajabadi pour A., and Dashti H. 2011. Effect of salinity and organic matters on chemical composition and root morphology of pistachio seedlings. Journal of Crops Improvement, 13(1): 31-42. (In Persian)
Rogiers S.Y., Greer D.H., Hatfield J.M., Orchard B.A., and Keller M. 2006. Mineral sinks within ripening grape berries (Vitis vinifera L.). Vitis, 45: 115- 23.
Sarkhosh A., Zamani Z., Fatahi R., and Ebadi A. 2006. RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Science Horticulture, 111: 24–29.
Salehi B., Bagherzadeh A., Ghasemi M., and Ebrahimi M. 2013. Effect of different amounts of humic humic acid on quality and quantity of tomato varieties (Lycopersium esculantium). Journal of Plant Production, 20(4): 189-198. (In Persian)
Sedaghat Kish Z., Moallemi N., Rahemi M., Khaleghi I., and Mortazavi S.M.H. 2013. The effect of urea and zinc sulfate solution on the storage and transfer of nitrogen and zinc elements in leaf and fruit of pomegranate Punica granatum L. (cv. ‘Rabab-e-Neyriz’). Horticultural Science of Iran, 34(2): 67-80. (In Persian)
Sepehr E., and Zebardast R. 2013. Effect of Humic Acid on Adsorption Behavior of Phosphorus in a Calcareous Soil. Journal of Water and Soil, 27(4): 720-731. (In Persian)
Singleton V.L., and Rossi J.L. 1965. Colorimetry of total phenolics with phosphomolybdic– phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16 (3): 144–158.
Shaaban S., Manal F., and Afifi M. 2009. Humic acid foliar application to minimize soil applied fertilization of surface-irrigated wheat. Journal of Agricultural Science, 5(2): 207-210.
Shehata S.A., Gharib A.A., El-Mogy M.M., Abdel-Gawad K.F., and Shalaby E.A. 2011. Influence of compost, amino and humic acids on the growth, yield and chemical parameters of strawberries. Journal of Medicinal Plants Research, 5: 2304-2308.
Song CZ., Liu M-Y., Meng J-F., Chi M., Xi Z-M., and Zhang Z-W. 2015. Promoting effect of foliage sprayed Zinc sulfate on accumulation of sugar and phenolics in berries of Vitis vinifera cv. Merlot growing on Zinc deficient soil. Journal of Molecules, 20: 2536-2554.
Soumare M., Tak G., and Verloo M.G. 2003. Effects of municipal solid waste compost and mineral fertilization on plant growth in two tropical agricultural soils of Mali. Bioresource Technology, 86: 15-20.
Srikanth K., Srinivasamurthy C.A., and Siddamarappa V.R. 2000. Direct and residual effect of enriched compost, vermicompost and fertilizerz on properties of an Alfisol. Journal of the Indian Society of Soil Science, 48(3):496-499.
Strategic Oversight Deputy, Office of the Technical Executive. 2009. Instructions for laboratory analysis of soil and water samples. 2009. Strategic Oversight Deputy, Office of the Technical Executive; Ministry of Agricultural Jihad, Soil and Water Research Institute, 278p. (In Persian)
Tadayon MS. 2015. Guidelines for feeding pomegranate. Agricultural Research and Education Center and Natural Resources, Fars Province, Iran, 56p. (In Persian)
Tehranifar A., and Mahmoodi Tabar S. 2009. Foliar application of potassium and boron during pomegranate (Punica granatum L.) fruit development can improve fruit quality. Horticulture, Environmental and Biotechnology, 50(3): 191-196.
Tehranifar A., Zarei M., Nemati Z., Esfandiyari B., and Vazifeshenas M.R. 2010. Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars. Scientia Horticulturae, 126: 180–185.
Turkmen O., Demir S., Sensoy S., and Dursun A. 2005. Effect of arbuscular mycorrhizal fungus and humic acid on the seedling development and nutrient content of pepper grown under saline soil conditions. Journal of Biosciences, 5(5):565-574.
Walkley A., and Black I.A. 1934. An examination degtijarf method for determination for role organic matter and proposed modification of the chromic acid titration method. Soil Science, 37:29–38.
Zandonadi DB., Canellas LP., and Facmana, AR. 2007. Indolacetic and humic acids induce lateral root development through a concerted plasmalemma and tonoplast H+ pumps activation. Planta, 225: 1583-1595.
Zaouay F., Mena P., Garcia-Viguera C., and Mars M. 2012. Antioxidant activity and physico-chemical properties of Tunisian grown pomegranate (Punica granatum L.) cultivars. Industrial Crops and Products, 40: 81– 89.