Evaluation of the effect of plant growth promoting bacteria and municipal waste compost on enzyme changes in black mulberry leaf

Document Type : Original Article

Authors

1 Ph. D Soil physics and conservation, Faculty of Agriculture, Guilan University.

2 2- Iran silkworm research center, Guilan, Rasht,

3 Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht,

4 silkworm research center, Guilan, Rasht,

5 Iran silkworm research center, Guilan, Rasht,

6 ran silkworm research center, Guilan, Rasht,

Abstract

Mulberry is a single-stemmed tree, growing in a variety of climates. Mulberry leaves are the main source of food for silkworm, which plays a key role in silk industry. In recent decades, since the use of chemical fertilizers has caused many environmental problems, the application of organic and bio-fertilizers has increased significantly. The current study aimed to investigate the impact of growth promoting bacteria and municipal waste on enzyme changes in mulberry leaves as well as soil phosphatase enzyme. For this research, 27 mulberry seedlings were collected from Iran Silk Research Centre. This research was conducted as a completely randomized design with three replications in the research greenhouse of Guilan University. In this study, the treatments used included two levels of municipal waste (two and four percent), two levels of Pseudomonas sp. inocula (106 and 5×106 cells per liter) and control sample. Catalase, polyphenol oxidase and superoxide dismutase, chlorophyll a and b in mulberry leaves were measured and also soil phosphatase was determined. The results indicated that the application of bacteria inocula led to the increment in the amount of soil phosphatase enzyme. The results also showed that the amount of superoxide dismutase enzyme were increased from 77.2 in the control treatment to 276 μmol/g of fresh leaf tissue in the higher level of compost and bacteria treatment (second level). Furthermore, the highest amount of this enzyme was equal to 0.16 (μmol · min−1 · g−1 wet weight), which was observed in the higher level of Pseudomonas sp. inoculum (5×106) along with the higher level of organic fertilizer (4%). In general, the results demonstrated that a combination of organic fertilizer and beneficial bacteria inocula can be used instead of chemical fertilizers to provide the elements required by the mulberry plant.

Keywords

References

Andrade M. C., Winemiller K. O., Barbosa P. S., Fortunati A., Chelazzi D., Cincinelli A., and Giarrizzo T. 2019. First account of plastic pollution impacting freshwater fishes in the Amazon: Ingestion of plastic debris by piranhas and other serrasalmids with diverse feeding habits. Environmental Pollution, 244, 766–773.
Ashton K., Holmes L., and Turner A. 2010. Association of metals with plastic production pellets in the marine environment. Marine Pollution Bulletin, 60(11), 2050–2055.
Book U. Y. 2014. Emerging issues update air pollution: World’s worst environmental health risk. United Nations Environment Programme.
Bouyoucos G. J. 1962. Hydrometer method improved for making particle size analyses of soils 1. Agronomy Journal, 54(5), 464–465.
Bradney L., Wijesekara H., Palansooriya K. N., Obadamudalige N., Bolan N. S., Ok Y. S., Rinklebe J., Kim K.-H., and Kirkham M. B. 2019. Particulate plastics as a vector for toxic trace-element uptake by aquatic and terrestrial organisms and human health risk. Environment International, 131, 104937.
Chen S., Tan Z., Qi Y., and Ouyang C. 2019. Sorption of tri-n-butyl phosphate and tris (2-chloroethyl) phosphate on polyethylene and polyvinyl chloride microplastics in seawater. Marine Pollution Bulletin, 149, 110490.
Cherian C., and Arnepalli D. N. 2015. A Critical Appraisal of the Role of Clay Mineralogy in Lime Stabilization. International Journal of Geosynthetics and Ground Engineering, 1(1), 1–20.
Cole J. C., Smith M. W., Penn C. J., Cheary B. S., and Conaghan K. J. 2016. Nitrogen, phosphorus, calcium, and magnesium applied individually or as a slow release or controlled release fertilizer increase growth and yield and affect macronutrient and micronutrient concentration and content of field-grown tomato plants. Scientia Horticulturae, 211, 420–430.
de Souza Machado A. A., Kloas W., Zarfl C., Hempel S., and Rillig M. C. 2018. Microplastics as an emerging threat to terrestrial ecosystems. Global Change Biology, 24(4), 1405–1416.
de Souza Machado A. A., Lau C. W., Kloas W., Bergmann J., Bachelier J. B., Faltin E., Becker R., Görlich A. S., and Rillig M. C. 2019. Microplastics can change soil properties and affect plant performance. Environmental Science and Technology, 53(10), 6044–6052.
Ekebafe L., Ogbeifun D., Biokemistri F. O.-, and 2011, undefined. 2011. Polymer applications in agriculture. Ajol.Info, 23(2), 81–89. https://www.ajol.info/index.php/biokem/article/view/77680.
Europe P. 2016. Plastics—The Facts 2016. An Analysis of European Latest Plastics Production, Demand and Waste Data.
Geyer R., Jambeck J. R., and Law K. L. 2017. Production, use, and fate of all plastics ever made. Science Advances, 3(7), e1700782.
Guo J., Huang X., Xiang L., Wang Y., Li Y., Li H., and Cai Q. 2020. Source, migration and toxicology of microplastics in soil. Environment International, 137(July 2019), 105263.
Havlin J. L. 2020. Soil: Fertility and Nutrient Management. Landscape and Land Capacity, 251–265.
Holmes L. A., Turner A., and Thompson R. C. 2012. Adsorption of trace metals to plastic resin pellets in the marine environment. Environmental Pollution, 160(1) 42–48.
Holmes L. A., Turner A., and Thompson R. C. 2014. Interactions between trace metals and plastic production pellets under estuarine conditions. Marine Chemistry, 167, 25–32.
Jalali M., Arian T. M., and Ranjbar F. 2020. Selectivity coefficients of K, Na, Ca, and Mg in binary exchange systems in some calcareous soils. Environmental Monitoring and Assessment, 192(2).
Lambert S., and Wagner M. 2018. Microplastics are contaminants of emerging concern in freshwater environments: An overview. Handbook of Environmental Chemistry, 58, 1–23.
Liu E. K., He W. Q., and Yan C. R. 2014. ‘White revolution’to ‘white pollution’—agricultural plastic film mulch in China. Environmental Research Letters, 9(9), 91001.
Liu S., Shi J., Wang J., Dai Y., Li H., Li J., Liu X., Chen X., Wang Z., and Zhang P. 2021. Interactions between Microplastics and Heavy Metals in Aquatic Environments: A Review. Frontiers in Microbiology, 12(April), 1–14.
Loeppert R. H., and Suarez D. L. 1996. Carbonate and gypsum. Methods of Soil Analysis: Part 3 Chemical Methods, 5, 437–474.
Mao R., Lang M., Yu X., Wu R., Yang X., and Guo X. 2020. Aging mechanism of microplastics with UV irradiation and its effects on the adsorption of heavy metals. Journal of Hazardous Materials, 393, 122515.
Nassr, M., Krupa, I., Ouederni, M., Krishnamoorthy, S. K., & Popelka, A. (2023). An Adhesion Improvement of Low-Density Polyethylene to Aluminum through Modification with Functionalized Polymers. Polymers, 15(4), 916.
Rhoades J. D., and Miyamoto S. 1990. Testing soils for salinity and sodicity. Soil Testing and Plant Analysis, 3, 299–336.
Richards L. A. 1954. Diagnosis and Improvement of. Saline and Alkali Soils. Handbook, 60.
Rillig M. C. 2012. Microplastic in terrestrial ecosystems and the soil? ACS Publications.
Rillig M. C., Ingraffia R., and Machado A. A. D. S. 2017. Microplastic Incorporation into Soil in Agroecosystems. 8(October), 8–11.
Rochman C. M., Browne M. A., Halpern B. S., Hentschel B. T., Hoh E., Karapanagioti H. K., Rios-Mendoza L. M., Takada H., Teh S., and Thompson R. C. 2013. Classify plastic waste as hazardous. Nature, 494(7436), 169–171.
Rochman C. M., Hentschel B. T., and Teh S. J. 2014. Long-term sorption of metals is similar among plastic types: implications for plastic debris in aquatic environments. PLOS One, 9(1), e85433.
Ruimin Q., Jones D. L., Zhen L., Qin L., and Changrong Y. 2019. Behavior of microplastics and plastic film residues in the soil environment: A critical. Science of the Total Environment, 134722.
Thomas G. W. 1996. Soil pH and soil acidity. Methods of Soil Analysis: Part 3 Chemical Methods, 5, 475–490.
Turner A., and Holmes L. A. 2015. Adsorption of trace metals by microplastic pellets in fresh water. Environmental Chemistry, 12(5), 600–610.
Wan Y., Wu C., Xue Q., and Hui X. 2019. Effects of plastic contamination on water evaporation and desiccation cracking in soil. Science of the Total Environment, 654, 576–582.
Wang J., Peng J., Tan Z., Gao Y., Zhan Z., Chen Q., and Cai L. 2017. Microplastics in the surface sediments from the Beijiang River littoral zone: Composition, abundance, surface textures and interaction with heavy metals. Chemosphere, 171, 248–258.
Wijesekara H., Bolan N. S., Bradney L., Obadamudalige N., Seshadri B., Kunhikrishnan A., Dharmarajan R., Ok Y. S., Rinklebe J., and Kirkham M. B. 2018. Trace element dynamics of biosolids-derived microbeads. Chemosphere, 199, 331–339.
Xu B., Liu F., Cryder Z., Huang D., Lu Z., He Y., Wang H., Lu Z., Brookes P. C., Tang C., Gan J., and Xu J. 2020. Microplastics in the soil environment: Occurrence, risks, interactions and fate–A review. Critical Reviews in Environmental Science and Technology, 50(21), 2175–2222.
Zhang G. S., Zhang F. X., and Li X. T. 2019. Effects of polyester microfibers on soil physical properties: Perception from a field and a pot experiment. Science of the Total Environment, 670, 1–7.
Zhou J., Wen Y., Marshall M. R., Zhao J., Gui H., Yang Y., Zeng Z., Jones D. L., and Zang H. 2021. Microplastics as an emerging threat to plant and soil health in agroecosystems. Science of the Total Environment, 787, 147444.
Zou J., Liu X., Zhang D., and Yuan X. 2020. Adsorption of three bivalent metals by four chemical distinct microplastics. Chemosphere, 248, 126064.

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History

  • Receive Date: 23 November 2021
  • Revise Date: 01 February 2022
  • Accept Date: 19 January 2022

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