Impact of Phosphate-Solubilizing Bacteria on the Microbial Activity and Inorganic Fractions of Phosphorus in Sugarcane Cultivated Calcareous Soil

Document Type : Original Article

Authors

1 Researcher, Department of Agronomy Research, Khuzestan Sugarcane Research and Training Institute, Ahvaz, Iran.

2 Director of Applied Research, Hakim Farabi Agro-Industry CO., Khuzestan, Iran

3 Head of Soil Science, Debal Khozaei Agro-Industry CO., Ahvaz, Iran.

4 Head of Water, Soil and Plant Laboratory Debal Khozaei Agro-Industry CO., Ahvaz, Iran.

5 Professor, Department of Soil Science , Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

10.30466/asr.2025.55605.1860

Abstract

Phosphate solubilizing bacteria are effective in improving phosphorus availability in calcareous soils by different mechanisms. The objective of this study was to investigate the effect of phosphate solubilizing bacteria inoculation on microbial activity, phosphorus availability, and inorganic phosphorus fractions in calcareous soil cultivated with second ratoon sugarcane. The study was conducted in a research field (CP73-21 variety) at the Dabal Khozaei Agro-industry in Khuzestan. The experiment followed a randomized complete block design with three treatments: 1- control (C), 2- inoculation with Enterobacter cloacae R33 (B33), and inoculation with a bacterial consortium (Enterobacter cloacae R33, Brevundimonas sp. Staphylococcus hominis 9E) (Bmix). Phosphate solubilizing bacteria were applied using the soil spraying method. Three months after the treatments inoculated, basal microbial respiration, microbial biomass carbon, soil available phosphorus, and chemical fractions of phosphorus in the soil were measured. The results indicated that phosphate solubilizing bacteria inoculation caused a significant increase in microbial respiration, microbial biomass carbon, and soil available phosphorus. In the B33 and Bmix treatments, available soil phosphorus concentrations were 52.5% and 24.6% higher than in the control treatment, respectively. B33 inoculation led to a significant increase in the Ca2-P and Ca8-P fractions and a decrease in Al-P and Ca10-P fractions in the soil. The proportion of inorganic phosphorus fractions in the control treatment was as follows: Ca10-P > Ca8-P > Fe-P > Al-P > Ca2-P, while in the B33 treatment, the trend was: Ca8-P > Ca10-P > Fe-P > Ca2-P > Al-P. The results revealed that the B33 treatment was more effective in enhancing phosphorus availability in the soil than the Bmix treatment. In general, the results of this study demonstrated that inoculation of Enterobacter cloacae R33 can effectively increase phosphorus availability in calcareous soil by converting phosphorus from less available fractions to more available forms.

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References

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Applied Soil Research
Volume 13, Issue 3 - Serial Number 3
December 2025
Pages 37-48

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History

  • Receive Date: 28 September 2024
  • Revise Date: 12 May 2025
  • Accept Date: 13 May 2025

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