Effect of Dual Inoculation with Pseudomonas fluorescens and Glomus Intraradices on Nutrient Uptake in Tomato Under Defferent Levels on Salinity

Document Type : Original Article

Authors

1 MSc Student, Department of Soil Science, University of Tabriz

2 Professor, Soil Science Department, Faculty of Agriculture, University of Tabriz

3 Assistant Professor, Soil Science Department, Faculty of Agriculture, University of Tabriz

4 Department of Soil Science, College of Agriculture, University of Tabriz

Abstract

The plants in symbiosis with Arbuscular Mycorrhizal Fungi (AMF) can absorb more nutrients and water which increases their resistant’s against environmental stresses such as drought or salinity. It is perceived that the presence of Plant Growth Promoting Rihzobacteria (PGPR) especially Pseudomonas in the rhizosphere of these plants has probably synergistic effect with mycorrhizal fungi. This experiment was performed to study the effect of single and dual inoculation of Pseudomonas fluorescens and Glomus intraradices on nutrient uptake in tomato variety Super strain B under salinity stress. A factorial experiment based on completely randomized block design with application of Glomus intraradices (including 2 levels; with inoculation (+AM) and without inoculation (-AM)), Pseudomonas fluorescens (including 3 levels; P. fluorescens strain Tabriz P. fluorescens strain Chao and without bacterial inoculation (control)) and four levels of salinity (S1:1.56, S2: 3 S3: 6 and S4: 9 dS m-1) was done in a pot culture with 3 replications. Mixed of different salts such as NaCl, CaCl2, MgSO4 and Na2SO4 were used to make salinity levels. Salinity treatments were daily exerted after two weeks of seedling and continued for 45 days. S1 (1.56 dS‌‌ ‌m-‌1) EC of the nutrient solution in distilled water was used as medium. Statistical analysis showed that concentration of nutrients such as phosphorus of shoot, potassium, calcium and magnesium of root and also root Ca/Na, root and shoot K/Na in PFT was higher than PFC and control treatment significantly (P<0.05). The sodium of root and chloride of shoot in the presence of PFC was higher than in PFT and control treatment. The concentrations of Na, Ca, Mg and Cl in plant tissues increased with increasing salinity, while P and K concentrations showed a decreasing trend. Based on the result obtained in this study, inoculating tomato plants with PFT improves salt tolerance index that cause favorable growth conditions for tomato plant.  

Keywords

References

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Applied Soil Research
Volume 1, Issue 2 - Serial Number 2
Vol: 1, No: 2
February 2014
Pages 45-60

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History

  • Receive Date: 08 March 2013
  • Revise Date: 02 March 2016
  • Accept Date: 28 August 2013

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