Role of Soil Microbes in Phytoremediation of Cadmium Contaminated Soils by onopordon (Onopordon acanthium L.)

Document Type : Original Article

Author

Department of Soil Science, Urmia University, Iran

Abstract

Microbial inoculation improves plants growth and increases their tolerance to environmental stresses and induces phytoremediation of heavy metals contaminated soils. In order to evaluate the role of some strains of AMF (a mixture of Glomus speciesincluding G. intraradices, G. mosseae and G. fasciculatum) and PGPR (a mixture of Pseudomonas speciesincludeing P. putida, P. fluorescens, and P. aeruginosa) in reclamation of cadmium (Cd) contaminated soils by Onopordon (Onopordon acanthium L) a factorial experiment based on a randomized complete block design and in three replications was carried out in greenhouse condition. The first factor was Cd concentration in four levels including 0, 10, 30 and 100 mg kg-1 and the second factor was microbial treatment in three levels including non-inoculation, and AMF or PGPR inoculation. A soil sample was spiked uniformly with Cd nitrate salt to create different Cd concentrations. The contaminated soils were then sterilized and subsequently inoculated with AMF and PGPR. Results showed that with increasing soil Cd concentration, colonization percent, and abundance of rhizobacteria, shoot biomass and shoot relative biomass significantlydecreased, while proline content and the shoot Cd concentration significantly increased (P ≤ 0.05). Mean Cd extracted in AMF and PGPR treatments were respectively 3.1 and 2.6 order of magnitude higher compared to the corresponding blank treatments. Therefore, it could be concluded that inoculation with species of AMF and PGPR can be a promising technique for enhancing the potential of onopordon plant in extraction of Cd from contaminated soils.
 

Keywords

References

References
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Applied Soil Research
Volume 2, Issue 1 - Serial Number 1
Vol: 2, No: 1
August 2014
Pages 59-70

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History

  • Receive Date: 24 August 2014
  • Revise Date: 08 March 2016
  • Accept Date: 16 January 2015

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