Effects of Two Species of Arbuscular Mycorrhizal Fungi on Leachates pH of Sorghum and Tomato in Vegetative Growth Period under Micronutrient Deficient Condition

Document Type : Original Article

Authors

1 Department of Soil Science, College of Water and Soil Engineering, University of Zabol. Iran.

2 Professors, Department of Soil Science, University of Tabriz. Iran

Abstract

Colonization of roots with arbuscular mycorrhizal fungi (AMF) often improves micronutrients uptake by most of the plants. Measurement of pH in mycorrhizae leachates is an evidence for acidification or alkalinization of rhizosphere and hyphosphere. Leachate pH is very important factor for assessment of micronutrient uptake by AMF. This experiment was laid out in factorial complete randomized block design with three replications. The first factor consists of arbuscular mycorrhizal fungi with Glomus etunicatum and Glomus intraradices species and control, the second factor was Rorison’s nutrient solution with zero, half and full strength of micronutrients, and the third factor was time of measurement of leachates pH that was include 45, 65 and 85 days after sowing. Tomato (Lycopersicon esculentum Miller) and sorghum (Sorghum bicolor L.) plants were grown in sterile perlite and were inoculated with either Glomus etunicatum or G. intraradices, while the control set was left un-inoculated. Rorison's nutrient solution with three levels of 0, half and full strength of micronutrients was applied to the pots during vegetative growth period. The pH of leachates Measured at 45, 65 and 85 days after sowing (DAS). Results show that, colonization of sorghum roots by G. etunicatum and G. intraradices fungi were 43 and 37%, respectively. On the contrary of sorghum plants, the mycorrhizal symbiosis was not observed in tomato plants. The pH of leachates was lower in mycorrhizal than non-mycorrhizal plants. G. intraradices were efficient than G. etunicatum in this respect. The reduction in leachate pH was induced at 0 levels of the micronutrients. 65 DAS, leachates had minimum amount of pH. In all of treatments, pH of leachates were higher than 7.6. It seems that, the main agent of this phenomenon is nitrate nutrition of plants, because nitrate is the most source of N in Rorison’s nutrient solution.

Keywords

References

References
Aliasgharzad N, Shirmohamadi E and Oustan S. 2009. Siderophore production by mycorrhizal sorghum roots under micronutrient deficient condition. Soil and Environment, 28: 119-123.
Angle JS, Heggo A and Chaney RL. 1989. Soil pH, rhizobia, vesicular-arbuscular mycorrhiza inoculation effect on growth and heavy metal uptake of alfalfa (Medicago sativa L.). Biology and Fertility of Soils, 8: 61-65.
Becard G, Douds DD and PefferPE. 1992. Extensive in vitro hyphal growth of vesicular-arbuscular mychorrhizal fungi in the presence of CO2 and flavonols. Applied and Environmental Microbiology, 58: 821-825.
Bienfait HF. 1985. Regulated redox processes at the plasmalemma of plant root cells and their function in iron uptake. Journal of Bioenergetics and Biomembranes, 17: 73- 83.
Bolan NS. 1991. A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Plant and Soil, 134: 189-207.
Cairney JW and Ashford AE. 1989. Reducing activity at the root surface in Eucaliptus pilularris- Pisolithus tinctorius ectomycorrhizas. Plant Physiology, 16: 99-105.
Cakmak I and Marschner H. 1990. Decrease in nitrate uptake and increase in proton release in zinc deficient cotton, sunflower and buckwheat plants. Plant and Soil, 129: 261-268.
Caris C, Hordt W, Hawkins HJ, Romheld V and George E. 1998. Studies of iron transport by arbuscular mycorrhizal hyphae from soil to peanut and sorghum plants. Mycorrhiza, 8: 35-39.
Clark RB and Zeto SK. 1996. Mineral acquisition by mycorrhizal maize grown on acid and alkaline Soil. Soil Biology and Biochemistry, 28: 1495-1503.
Ding X, Sui X, Wang F, Gao J, He X, Zhang F, Yang J, Feng G. 2012. Synergistic interactions between Glomus mosseae and Bradyrhizobium japonicum in enhancing proton release from nodules and hyphae. Mycorrhiza, 22: 51-58.
Dinkelaker B, Römheld V and Marschner H. 1989. Citric acid excretion and precipitation of calcium citrate in the rhizosphere of white lupin (Lupinus albus L). Plant, Cell and Environment, 12: 285-292.
Gianinazzi-Pearson V, Branzanti B and Gianinazzi S. 1989. In vitro enhancement of spore germination and early hyphal growth of a vesicular-arbuscular mycorrhizal fungus by host root exudates and plant flavonoids. Symbiosis, 7: 243-255.
Giovannetti M and Mosse B. 1980. An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytologist, 84: 489-500.
Goh TB, Banerjee MR, Tu S and Burton DL. 1997. Vesicular arbuscular mycorrhizae-mediated uptake and translocation of P and Zn by wheat in a calcareous soil. Canadian Journal of Soil Science, 77: 339-346. 
Grace C and Stribley DP. 1991. A safer procedure for routine staining of vesicular arbuscular mycorrhizal fungi. Mycological Research, 95: 1160-1162.
Kormanik PP and McGraw AC. 1982. Quantification of VA mycorrhizae in plant roots. In: SchenkNC (ed.). Methods and principles of mycorrhizal research. American Phytopathological Society, Saint PaulMinnesota, pp: 37-45.
Landsberg EC. 1981. Organic acid synthesis and release of hydrogen ions in response to Fe deficiency stress of mono and dicotyledonous plant species. Journal of Plant Nutrition, 3: 579-591.
Lee YJ and George E. 2005. Contributions of mycorrhizal hyphae to the uptake of metal cations by cucumber plants at two levels of phosphorus supply. Plant and Soil, 278: 361-370.
Li X, George E and Marschner H. 1991. Phosphorus depletion and pH decrease at the root–soil and hyphae–soil interfaces of VA mycorrhizal white clover fertilized with ammonium. New Phytologist, 119: 397-404.
Manoharan PT, Pandi M, Shanmugaiah V, Gomathinayagam S and Balasubramanian N. 2008. Effect of vesicular arbuscular mycorrhizal fungus on the physiological and biochemical changes of five different tree seedlings grown under nursery conditions. African Journal of Biotechnology, 7: 3431-3436.
Merryweather JW and Fitter AH. 1991. Techniques in arbuscular mycorrhiza research. York Mycorrhiza Research Group, UK, 973p.
Mihashi S, Mori S and Nishizawa N. 1991. Enhancement of ferric-mugineic acid uptake by iron deficient barley roots in the presence of excess free mugineic acid in the medium. Plant and Soil, 130: 135-141.
Miller AJ and Smith SJ. 1996. Nitrate transport and compartmentation in cereal root cells. Journal of Experimental Botany, 47: 843-854.
MiyasakaSC, Habte M, Friday JB and Johnson EV. 2003. Manual on arbuscular mycorrhizal fungus production and inoculation techniques. Crop and Soil Management, 5:1-4.
Mwangi MW, Monda EO, Okoth SA and Jefwa JM. 2011. Inoculation of tomato seedlings with Trichoderma harzianum and arbuscular mycorrhizal fungi and their effect on growth and control of wilt in tomato seedlings. Brazilian Journal of Microbiology, 42: 508-513.
Powell CL and Bagraraj DJ. 1984. VA Mycorrhiza. CRC Press, Florida, USA, 234p.
Remy W, Taylor TN, Hass H and Kerp H. 1994. Four hundred-million-year-old vesicular arbuscular mycorrhizae. Plant Biology, 91: 11841-11843.
Ric DV, Lubbrding CHJ and Bienfait HF. 1986. Rhizosphere acidification as a response to iron deficiency in bean Plants. Plant Physiology, 81: 842-846.
Romheld V, Muller C and Marschner H. 1984. Localization and capacity of proton pumps in roots of intact sunflower plants. Plant Physiology, 76: 603-606.
Romheld V. 1991. The role of phytosiderophores in acquisition of iron and other micronutrients in graminaceous species: An ecological approach. Plant and Soil, 130: 127-134.
Rosenfield CL, Reed DW and Kent MW. 1991. Dependency of iron reduction on development of unique root morphology in Ficus benjamina L. Plant Physiology, 95: 1120-1124.
Sanders FE and Tinker PB. 1973. Phosphate flow into mycorrhizal roots. Journal of Pest Science, 4: 385-395.
Schreiner PR. 2007. Effect of native and non native arbuscular mycorrhizal fungi on growth and nutrient uptake of ‘Pinot noir’ (Vitis vinifera L.) in two soils with contrasting levels of phosphorus. Applied Soil Ecology, 36: 205-215.
SchumakerKS and Heven S. 1987. Decrease of pH gradients in tonoplast vesicles by N03- and Cl-: Evidence for H+-coupled anion transport. Plant Physiology, 83: 490-496.
SubramanianKS and Charest C. 1997. Nutritional, growth, and reproductive responses of maize (Zea mays L.) to arbuscular mycorrhizal inoculation during and after drought stress at tasselling. Mycorrhiza, 7: 25-32.
Takagi S, Nomoto K and Takemoto T. 1984. Physiological aspects of mugineic acid, a possible phytosiderophore of graminaceous plants. Journal of Plant Nutrition, 7: 469-477.
Yao Q, Li X, Feng G and Christie P. 2001. Mobilization of sparingly soluble inorganic phosphates by the external mycelium of an arbuscular mycorrhizal fungus. Plant and Soil, 230: 279-285.
Applied Soil Research
Volume 2, Issue 1 - Serial Number 1
Vol: 2, No: 1
August 2014
Pages 71-83

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History

  • Receive Date: 06 April 2014
  • Revise Date: 08 March 2016
  • Accept Date: 10 November 2014

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