Effect of different strains of Azotobacter chroococcum on morphophysiologic traits and yield components of rapeseed (Brassica napus L)

Document Type : Original Article

Authors

Abstract

In order to evaluate the effect of Azotobacter chroococcum different strains on morphophysiologic characteristics, seed yield and yield components of fall rapeseed (Brassica napus L.), an experiment was conducted in Rasht. The experimental design was completely randomized block, with three replication. Investigated treatments were including seed inoculation with Azotobacter different strains (6, 9, 11, 12, 13, 14, 15, 16, 19, 21, 23, 25, 28, 35 and 38) and chemical fertilizer application (full Urea fertilizer application) and without seed inoculation conditions (control). Results showed that Azotobacter strains significantly affected on all traits except plant height, weight of 1000 seeds, stem diameter and number of secondary branches. The most distance of the first secondary branch until ground obtained by A. chroococcum strain 35, as A. chroococcum strain 14 caused the highest of silique length, dry weight, seed yield and number of seeds per silique. The most leaf erea, dry weight, seed yield and yield components caused by A. chroococcum strain 12. Generally, A. chroococcum strain 12, A. chroococcum strain 14 and A. chroococcum strain 9 had the more positive effect on traits compared with other strains. Results of this experiment showed the use of A. chroococcum increase quantitative and qualitative characteristics of canola in relation to full Urea fertilizer application and the savings in fertilizer consumption and reduce environmental degradation, therefore can be used as supplementary of chemical fertilizer.

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Ahmad, F., Ahmad, I., and Khan, M. S. (2005). Indole acetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turkish Journal of Biology, 29(1), 29-34.
Ahmed, A. G., Orabi, S., and Gomaa, A. M. (2010). Bio-organic farming of grain sorghum and its effect on growth, physiological and yield parameters and antioxidant enzymes activity. Research Journal of Agriculture and Biological Science, 6(3), 270-279.
Al-Noaim, A. A., and Hamad, S. H. (2004). Effect of bio-fertilization along with different levels of nitrogen fertilizer application on the growth and grain yield of Hassawi rice (Oryza sativa L.). Scientific Journal of King Faisal University (Basic and Applied Sciences), 5(2), 1425.
Amirabadi, M., Borji, M., Ardakani, M. R., and Rejali, F. Determination of Efficiency of Mycorrhiza and Azotobacter in Uptake of Microelements, Zn, Cu and Fe under Different Levels of Phosphorus in Corn Hybrid of KSC 704. World Journal of Agriculture Science, 2:16-20.
Ananthanaik, T., Earanna, N., and Suresh, C.K., (2006). Influence of Azotobacter chroococcum strains on growth and biomass of adathoda vasica Nees. Agronomy and Crop Science, 178, 164-171.
Asghar, H. N., Zahir, Z. A., and Arshad, M. (2004). Screening rhizobacteria for improving the growth, yield, and oil content of canola (Brassica napus L.). Crop and Pasture Science, 55(2), 187-194.
Ashrafuzzaman, M., Akhtar, H., and Razi, Ismail, M. (2009). Efficiency of plant growth promoting bacteria for the enhancement of rice growth. African Journal of Biotechnology, 8, 1247-1252.
Azadchegani, M.R., and Bohrani, M.J., (2003). The effect of nitrogen and planting day on yield and yield components of rapeseed (Brassica napus L.). In: proceeding of 7th Iranian Congress in Agronomy and Plant Breeding, (In Persian).
Ehteshami, S.M.R., AminDeldar, Z., and Khavazi, K. (2010b). Effect of Pseuodomonas sp. bacteria on germination characteristics and seedling growth of rice cultivars. Proceedings of the 11th Iranian Congrees in Agronomy and Plant Breeding, Shahid Beheshti University, Tehran, Iran, (In Persian).
Ehteshami, S.M.R., AminDeldar, Z., and Khavazi, K., (2010a). Effect of Pseuodomonas sp. spraying on quantitative characteristics and yield components of rice cultivars. Proceedings of the 11th Iranian Congrees in Agronomy and Plant Breeding, Shahid Beheshti University, Tehran, Iran, (In Persian).
Gaur, AC. (2001a). Effects of Azotobacter isolation on the yield of canola (Brassica napus L.): Laboratory experiment. Indian Society of Soil Science, 40, 19-22.
Gholami, A., Shahsavani, S., and Nezarat, S. (2009). The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. World Academy of Science, Engineering and Technology, 49.
Gholizadeh, A., Amin, M.S.M., Anuar, A.R., and Aimrun, W. (2009). Evaluation of spad chlorophyll meter in two different rice growth stage and its temporal variability. European Journal of Scientific Research, 37, 591-598.
Glick, B.R., Patten, C.L., Holguin, G., and Penrose, D.M. (1999). Biochemical and genetic mechanisms used by plant growth promoting bacteria. London: Imperial College Press.
Habekotte, B. (1993). Quantitative analysis of pod formation, seed set and seed filling in winter oilseed rape (Brassica napus) under field crop condition. Field Crop Research, 35, 21-33.
Hanson, W.C. (1950). The photometric determination of phosphorus in fertilizers using the phosphor vanadomolybdate complex. Journal of Science in Food and Agriculture, 1, 172-173.
Khavazi, K., Asadi, Rahmani, H., and Malakouti, M.J., (2005). Necessity of biofertilizer industrial production in the country (Collection of papers-Second Edition). Research Institute of Soil and Water, Agricultural Research, Education and Extension Organization, Ministry of Agriculture, 439p. (In Persian).
Mahato, P., Badoni, A., and Chauhan, J.S., (2009). Effect of Azotobacter and nitrogen on seed germination and early seedlind growth in tomato. Researcher, 1(4). http: www. Sciencepub. Net.
Mahfouz, S.A., and Sharaf-Eldin, M.A. (2007). Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics, 21, 361-366.
Mahmoodi, H., Khosravi, H., and Asgharzadeh, A. (2004). The role of Azotobacter biofertilizer in dryland wheat yield. Proceedings of the 8th Iranian Congrees in Agronomy and Plant Breeding, Guilan University, Rasht, Iran. (In Persian)
Mcgregor, D.I. (1988). Glucosinolate content of development rapeseed (Brassica napus L. ’Midas’) seedlings. Canadian Journal of Plant Science, 68, 367-580.
Megawer, E. A., and Mahfouz, S. A. (2010). Response of Canola (Brassica napus L.) to Biofertilizers under Egyptian conditions in newly reclaimed soil. International Journal of Agriculture Sciences, 2(1), 12.
Mendham, A.J., Shipway, P.A., and Scott, R.K. (1991). The effects of delayed sowing and wheather on growth, development and yield of winter oilseed rape (Brassica napus L). Journal of Agricultural Science, 96, 389-416.
Mirzakhani, M., Ardakani, M.R., Aeene, Band, A., Shirani, Rad, A.H., and Rejali, F. (2009). Effects of dual inoculation of Azotobacter and mycorrhiza with nitrogen and phosphorus fertilizer rates on grain yield and some of characteristics of spring safflower. International Journal of Environmental Science and Engineering, 1, 39-43.
Mohammadi, N. (2009). Effect of different levels of water stress on some physiological and morphological characteristics in two spring canola cultivars (Brassica napus L.). MSc Thesis of Agronomy, Tehran University, (In Persian).
Mrkovacki, N., and Milic, V. (2001). Use of Azotobacter chroococcum as potential useful in agricultural application. Annual Microbiology, 51, 145-158.
Narula, N., Kumar, V., Behl, R.K., Deubel, A., Gransee, A., and Merbach, W. (2000). Effect of P-solubilizing Azotobacter chroococcum on N, P and K uptake in P-responsive wheat genotypes grown under greenhouse conditions. Journal of Plant Nutrition, 163, 393-398.
Naseri, F. (1991). Oil seeds (Translation). Astan Ghods Razavi Press, 823p. (In Persian).
Nelson LM. 2004. Plant growth promoting rhizobacteria (PGPR): Prospects for new inoculants. Crop Management Doi:10.1094/CM-2004-0301-05-RV.
Nielsen, D.C. (1997). Water use and yield of canola under dry land conditions in central Great Plains. Journal of Production and Agriculture, 10, 307-313.
Ozer, H. (2003). Sowing date and nitrogen rates effects on growth, yield and yield components of two summer rapeseed cultivars. European Journal of Agronomy, 19, 453-463.
Pallai, R. (2005). Effect of Plant Growth-Promoting Rhizobacteria on canola (Brassica napus L.) and lentil (Lens culinaris Medik.) plants. Head of the Department, Applied Microbiology and Food Science. University of Saskatchewan.
Piromyou, P., Buranabanayat, B., Tantasawat, P., Tittabutr, P., Boonkerd, N., and Teaumroong, N. (2011). Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand. European Journal of Soil Biology, 47, 44-54.
Rokhzadi, A., Asgharzadeh, A., Darvish, F., Nourmohamadi, G., and Majidi, E. (2008). Influence of plant growth promoting rhizobacteria on dry matter accumulation and yield of chickpea (Cicer arietinum L.) under field conditions. American-Eurasian Journal Agriculture and Environmental Science, 3, 253-257.
Roshdi, M., Rezadoust, S., Khalili, Mahalleh, J., and Hajihasani, Asl, N. (2009). Effect of biofertilizer on yield and yield components of three oil sunflower cultivars. Journal of Agricultural Sciences, Islamic Azad University, Tabriz branch, 3(10), 11-24, (In Persian).
ShiraniRad, A.H., and Dehshiri, A. (2003). Guide for canola (planting, careful and harvesting). Assistance of Training and Equipping Human Resources, Ministry of Agriculture, Agricultural Education Press, 48p. (In Persian).
Sirjani, A., Farahbakhsh, H., Ravari, Z., Pasandipour, N., and Karami, A. (2011). Investigation the effect of biofertilizer consumption, zinc sulfate and nitrogen fertilizer on quantitative and qualitative yield of wheat. Iranian Journal of Soil Research (Soil and Water Sciences), 25(2), 125-135, (In Persian).
Stijn, S., Vanderleyden, J., and Okon, Y. (2009). Chapter 7 Plant Growth-Promoting Actions of Rhizobacteria. Advances in Botanical Research, 51, 283-320.
Timmusk, S. (2003). Mechanism of action of the plant growth promoting Bacterium Paenibacillus polymixa. Acta Universitatis Upsaliensis. Comprehensive Summaries of Upsala Dissertations from the Faculty of Science and Technology 908, 40 pp. Uppsala. ISBN 91-554-5802-5.
Tommy, A.M., and Evans, E.J. (1992). Analysis of post-flowering compensatory growth in rape (Brassica napus L) winter oilseed. Journal of Agricultural Science, 118, 301-308.
Turan, M., Gulluce, M., Karadayi, M., Baris, O., and Sahin, F. (2011). Role of soil enzymes produced by PGPR strains in wheat growth and nutrient uptake parameters in the field conditions. Current Opinion in Biotechnology, 22, 15–152.
Yasari, E., Esmaeili, M.A., Mozafari, S., and Alashti, M. (2009). Enhancement of growth and nutrient uptake of rapeseed (Brassica napus L.) by applying mineral nutrients and biofertilizaers. Pakistan Journal of Biological Science, 12, 127-133. 
Yosefi, K., Galavi, M., Ramrodi, M., and Mousavi, R. (2011). Effect of bio-phosphate and chemical phosphorus fertilizer accompanied with micronutrient foliar applicatin on growth, yield and yield components of maize (single cross 704). Australian Journal of Crop Science, 5: 175-180.
Zahir, A.Z., Arshad, M., and Frankenberger, W.F. (2004). Plant growth promoting rhizobacteria: application and perspectives in agriculture. Advances in Agronomy, 81, 97-168.
Zaidi, A., Khan, M.S., and Amil, M. (2003). Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). European Journal of Agronomy, 19,15-21.