Document Type : Original Article



Anise Hyssop (Agastache foeniculum) is a perennial and aromatic herb plant, belonging to the Lamiaceae family. Its essential oil has antifungal and antibacterial properties and used in food and pharmaceutical industries. To study the effects of foliar application of urea and amino acids on some morpho-physiological and biochemical characteristics of anise hyssop, a pot experiment was conducted in completely randomized design with four replications. The treatments were foliar application of urea (0.5, 1 and 2 g/l), aspartic acid (100, 200 and 300 mg/l), glutamic acid (100, 200 and 300 mg/l) and control (no nitrogen application). The results showed that foliar application of urea and amino acids had significant effects on growth, physiological and biochemical parameters. The highest and the lowest amounts of plant height, leaf number and area, stem diameter, fresh and dry weight of leaves and stems were obtained in 2 g/l of urea and control treatments, respectively. Foliar application of urea and amino acids increased photosynthesis pigments, proline, total soluble sugars, total protein and nitrogen content of leaves compared to control. Total protein, total soluble sugars, proline, leaf chlorophyll and nitrogen content increased as the concentration of amino acids increased. Overall, the findings of this study showed that foliar application of 2 g/l of urea was the most effective treatment for the studied characteristics however amino acids through improvement of growth and physiological characteristics can be used as new sources of nitrogen for plant nutrition.


Abd El-Aziz N.G., and Balbaa L.K. 2007. Influence of Tyrosine and zinc on growth, flowering and chemical constituents of Salvia farinacea plants. Journal of Applied Sciences Research, 3(11): 1479-1489.
Alizadeh Sahzabi A., Sharifi Ashorabadi E., Shiranirad A.H., Bigdeli M., and Abaszadeh B. 2007. The effects of different methods and levels of using nitrogen on some quality and quantity characteristics of Satureja hortensis L. Iranian Journal of Medicinal and Aromatic Plants, 23(3): 416-431. (In Persian)
Ameri A., Nasiri Mahallati M., and Rezvani P. 2007. Effects of different nitrogen levels and plant density on flower, essential oils and extract production and nitrogen use efficiency of Marigold (Calendula officinalis). Iranian Journal of Field Crops Research, 5(2): 315-325. (In Persian)
Ashrafi S. 2014. Foliar spray effects of urea and some amino acids on morphological, physiologicaland biochemical characteristics of costmary (Tanacetum balsamita L.). M.Sc. Thesis, Urmia University, Iran. 103 p. (In Persian)
Attoa G.W., Wahba E., and Farahat A.A. 2002. Effect of some amino acids and sulphur fertilization on growth and chemical composition of Iberis amara L. plants. Egyptian Journal of Horticulture, 29:17-37.
Bojovic B., and Stojanovic J. 2005. Chlorophyll and carotenoid content in wheat cultivars as a function of mineral nutrition. Archives of Biological Science Belgrade, 57(4): 283-290.
Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Charles D.J., Simon J.E., and Widrlechner M.P. 1991. Characterization of essential oil of Agastache species. Journal of Agricultural and Food Chemistry, 39: 1946-1949.
Devlin R.M. 1969. Plant Physiology. 2nd Ed., S. Muthiah at Tamil and Printers and Traders Pvt. Ltd., Madra, India. 164 p.
El-Naggar A.A.M., Adam A.I., and El-Tony F.E.Z.H. 2013. Response of Longiflorum X Asiatic Hybrid Lilium plants to foliar spray with some amino acids. Alex. Journal of Agricultural Research, 58(3): 197-208.
Gamal El-Din K. M., Tarraf A.Sh., and Balbaa A. 1997. Physiological studies on the effect of some amino acids and microelements on growth and essential oil content in lemon grass (Cymbopogon citrates Hort.). Journal of Agricultural Science Mansoura University, 22(12): 4229-4241.
Govind S., and Prasad A. 1982. Effect of nitrogen on fruit-set, fruit drop and yield of sweet orange.   Punjab Horticultural Journal, 22(1/2): 15-20.

Gross J. 1991. Pigments in Vegetables. Van Nostrand Reinhold, New York, 351 p.

Guler S., Ibrikci H., and Buyuk G. 2006. Effects of different nitrogen rates on yield and leaf nutrient contents of drip-fertigated and greenhouse-grown cucumber. Asian Journal of Plant Sciences, 5(4): 657-662.
Hassanein R.A.M.2003. Effect of some amino acids, trace elements and irradiation on fennel (Foeniculum vulgare L.). Ph.D. Thesis, Faculty of Agriculture, Cairo University, Egypt.
Irigoyen J.J., Emerich D.W., and Sanchez-Diaz M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84: 55-60.
Kamar M.E., and Omar A. 1987. Effect of nitrogen levels and spraying with aminal-forte (amino acids salvation) on yield of cucumber and potatoes. Journal of Agricultural Science Mansoura University, 12(4): 900 - 907.
Karima A., Gamal El-Din K.M., and Abdel-Wahed M.S.A. 2005. Effect of some amino acids on growth and essential oil content of chamomile plant. International Journal of Agriculture and Biology, 7(3): 376-380.
Lichtenthaler H.K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350-382.
Mallavarapu G.R., Kulkarni R.N., Baskaran K., and Ramesh S. 2004. The essential oil composition of anise hyssop grown in India. Flavour and Fragrance Journal, 19: 351-353.
Mardaninejad S.H., Khold Barin B., Sadat Y., Moradshahi A., and Vazir Pour M. 2005 Vegetative behavior change and the amount of essential oil of lavender (Lavandula officinalis) in response to different amounts of ammonium nitrate. Iranian Journal of Medicinal and Aromatic Plants, 19: 16-35. (In Persian)
Meawad A.A., Awad A.E., and Afify A. 1984. The combined effects of N-fertilization and growth regulators on chamomile plants. Acta Horticulturae, 144: 123-134.
Meek J.C. 1974. Chlorophylls. In: Stewart WDP, (ed.) "Algal Physiology and Biochemistry." Blackwell, London, pp: 161-75.
Milad S.M.N. 1998. Effect of tryptophan and methionine on the productivity of two Mentha species and American Ocimum plants. M.Sc. Thesis, Faculty of Agriculture, Cairo University, Egypt.
Moursy H.A., Hussein S.M., and El-Bahar K.M. 1988. Effect of some alkaloid precursors on the growth and alkaloid production of Datura stramonium L. cultured in vitro. Egyptian Journal of Botany, 31: 153-165.
Omer E.A., Said-Al Ahl H.A.H., El Gendy A.G., Shaban Kh.A., and Hussein M.S. 2013. Effect of amino acids application on production, volatile oil and chemical composition of chamomile cultivated in saline soil at sinai. Journal of Applied Sciences Research, 9(4): 3006-3021.
Omidbaigi R. 2014. Production and processing of medicinal plants. Astane Gods Razavi Press, Vol1, 347p. (In Persian)
Omidbaigi R., and Mahmoodi M. 2010. Effect of Irrigation Regimes on the Essential Oil Content and Composition of Agastache foeniculum. Journal of Essential Oil Bearing Plants, 13(1): 59-65.
Page A.L. 1982. Methods of soil analysis. Part 2: Chemical and microbiological properties. 2nd Eds. Soil Science Society of America, Book Series 5, Madison, WI.
Paquin R., and Lechasseur P. 1979. Observations sur une methode de dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany, 57(18): 1851-1854.
Phillips I.D.J. 1971. Introduction to the Biochemistry and Physiology of Plant Growth Hormones. Mc Graw-Hill Book Company, New York, 173p.
Qranjik A., and Galeshi S. (2001). Effect of nitrogen spray on yield and yield component of wheat. Agriculture and Natural Resource Journal, 8(2): 87- 98.
Salisbury F.B., and Ross C.W. 1992. Plant physiology. 4nd Eds, Wadsworth Publishing Company, Belmont, California, USA, 682 p.
Wojcik S.M., Rhee J.S., Herzog E., Sigler A., Jahn R., Takamori S., Brose N. and Rosenmund C., 2004. An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size. Proceedings of the National Academy of Sciences of the United States of America, 101 (18), pp.7158-7163.