تاثیر هیومیک اسید بر ویژگی های رویشی، جذب فسفر و پتاسیم و رنگدانه های فتوسنتزی دانهال های پسته تحت تنش خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم و مهندسی خاک، دانشکده کشاورزی،دانشگاه شهید چمران اهواز

2 دانشجوی دکتری علوم خاک، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

3 دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

کمبود آب عامل عمده محدود کننده رشد و تولید پسته در خاک های مناطق خشک و نیمه خشک ایران می باشد. هدف از این پژوهش بررسی تاثیر کاربرد هیومیک اسید بر ویژگی های رویشی ریشه و اندام هوایی، رنگدانه های فتوسنتزی و جذب فسفر و پتاسیم توسط دانهال های پسته در شرایط تنش خشکی بود. این پژوهش در گلخانه به صورت آزمایش فاکتوریل با دو فاکتور تنش خشکی در 3 سطح (شاهد یا 80 درصد، 60 درصد و 40 درصد ظرفیت زراعی) و هیومیک اسید در 3 سطح (شاهد، 1 و 2 گرم هیومیک اسید در کیلوگرم خاک)، در قالب طرح بلوک های کامل تصادفی در سه تکرار انجام شد. نتایج نشان داد با افزایش تنش خشکی در خاک، سطح برگ، وزن خشک برگ و ساقه، وزن خشک کل ریشه و اندام هوایی، محتوای کلروفیل و غلظت و مقدار فسفر جذب شده در ریشه و اندام هوایی کاهش یافت، درحالی که مقدار کاروتنوئیدها و غلظت پتاسیم در ریشه و اندام هوایی افزایش یافت. کاربرد هیومیک اسید سبب افزایش معنی دار طول بخش هوایی (17 درصد)، سطح برگ (39 درصد)، وزن خشک ریشه (31 درصد)، وزن خشک برگ (16 درصد)، وزن خشک ساقه (28 درصد)، وزن خشک کل گیاه (19 درصد)، کلروفیل کل (37 درصد)، کاروتنوئیدها (19 درصد)، غلظت فسفر در ریشه (10 درصد) و اندام هوایی (23 درصد) و مقدار پتاسیم جذب شده در ریشه (11 درصد) و اندام هوایی (22 درصد) شد. بنابراین می توان نتیجه گیری کرد که کاربرد هیومیک اسید (به ویژه سطح 2 گرم هیومیک اسید در کیلوگرم خاک) می تواند با بهبود محتوای رنگدانه های فتوسنتزی، و افزایش جذب عناصر غذایی فسفر و پتاسیم در ریشه و اندام هوایی، سبب بهبود رشد دانهال های پسته و افزایش بردباری آن ها در شرایط تنش خشکی می شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Humic Acid on Growth Characteristics, Phosphorous and Potassium Uptake and Photosynthesis Pigments of Pistachio Seedlings under Drought Stress

نویسندگان [English]

  • abdolamir moezzi 1
  • naser rashidi 2
  • Afrasyab Rahnama 3
1 Associate Professor, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz
2 Ph.D Student of Soil Science, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz
3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

Water deficit is the main growth-limiting factor for pistachio (Pistacia vera L.) production in semiarid and arid soils of Iran. The objective of this study was to evaluate the effect of humic acid application on root and shoot growth characteristics, phosphorus (P) and potassium (K) uptake and photosynthetic pigments of P. vera L. seedlings under drought stress condition. This study was carried out in greenhouse condition as a factorial experiment based on a randomized complete block design with two factors including drought stress (in three levels: 80% (control), 60% and 40% of field capacity moisture content) and humic acid (in three levels including control, 1 g HA kg-1 soil and 2 g HA kg-1 soil) in three replications. Results indicated that leaf area, dry weights of leaf and stem, shoot and root dry weights, chlorophyll contents and shoot and root P content decreased by increasing of soil drought levels, while the reverse result was found in carotenoids and shoot and root K concentrations. application of humic acid leads to significant increase (P≤0.05) in shoot length (17 %), leaf area (39 %) root dry weight (31 %), leaf dry weight (16 %), Stem dry weight (28 %), total plant dry weight (19 %), total chlorophyll (37 %), carotenoids (19 %), root P concentration (10 %), shoot P concentration (23 %) and K content in root (11 %) and shoot (22 %) in all moisture levels. It can be concluded that application of humic acid (especially at level of 2 g HA kg-1 soil) results in improve of the plant growth properties and tolerance to drought stress due to increase of photosynthesis pigments and enhance of shoot and root P and K contents.

کلیدواژه‌ها [English]

  • Carotenoid
  • Chlorophyll
  • growth
  • nutrients
  • Water deficit
Abbaspour H., Saeidi-Sar S., Afshari H. and Abdel-Wahhab M. A. 2012. Tolerance of mycorrhiza infected pistachio (Pistacia vera L.) seedling to drought stress under glasshouse conditions. Journal of Plant Physiology, 169(7): 704-709.

Akbarpour M., Khavari-Nejad R.A., Moumeni A., and Najafi F. 2016. Molecular and physiological performance in response to drought stress in Iranian rice cultivars. Russian Journal of Plant Physiology, 63(1): 158-165.

Amini B., Farahbakhsh M., and Kianirad M., 2017. Study on the Effects of Humic Acid-Urea Fertilizers Application on Some Agronomic Characteristics of Maize (Zea Mays L.). Applied Soil Research, 5(2): 31- 40). In Persian).

Asik B.B., Turan M.A., Celik H., and Katkat, A.V. 2009. Effect of humic substances to dry weigth and mineral nutrients uptake of wheat on saline soil conditions. Asian Journal of Crop Science, 1(2): 87-95.

Arnon D.T. 1949. Copper enzymes in isolation chloroplast phenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-15.

Bhatt R.M., and Srinivasa-Rao N.K. 2005. Influence of pod load on response of okra to water stress. Indian Journal Plant Physiology, 10: 54-59.

Burd G.I., Dixon, D.G., and Glick, B.R. 2010. A plant growth-promoting bacterium that decreases nickel toxicity in seedlings. Applied and Environmental Microbiology, 64: 3663-3668.

Canellas L.P., Silva S.F., Olk D.C., and Olivares F.L. 2015 Foliar application of plant growth-promoting bacteria and humic acid increase maize yields. Journal of Food, Agriculture and Environment, 13: 131-138.

Carter M.R., and Gregorich E.G. 2008. Soil sampling and methods of analysis (2nd ed). CRC Press. Boca Raton. FL. 1204p.

Dalal V.K., and Tripathy B.C. 2012. Modulation of chlorophyll biosynthesis by water stress in rice seedlings during chloroplast biogenesis. Plant Cell and Environment, 35: 1685-1703.

Delshadi S., Ebrahimi M., and Shirmohammadi E. 2017. Effectiveness of plant growth promoting rhizobacteria on Bromus tomentellus Boiss seed germination, growth and nutrients uptake under drought stress. South African Journal of Botany, 113: 11-18.

Esmaeilpour A., Van Labeke M.C., Samson R., Boeckx P., and Van Damme, P. 2016. Variation in biochemical characteristics, water status, stomata features, leaf carbon isotope composition and its relationship to water use efficiency in pistachio (Pistacia vera L.) cultivars under drought stress condition. Scientia Horticulturae, 211: 158-166.

Fahimi kuyerdi F., and Shamshiri, M.H. 2016. Comparison of photosystem II efficiency in four Pistachio rootstocks under drought stress using chlorophyll fluorescence technique. Journal of Plant Process and Function, 5 (17): 95-108. (In Persian).

FAO, O., 2012. http://faostat.fao.org/site/339/default.aspx.

Ferrara G., Pacifico A., Simeone P., and Ferrara, E. 2008. Preliminary study on the effects of foliar applications of humic acids on ‘Italia’ table grape. Journal International des Sciences de la Vigne et du Vin, 42: 79-87.

Galindo A., Collado-González J., Griñán I., Corell M., Centeno A., Martín-Palomo M. J., Girón I. F., Rodríguez P., Cruz Z. N., Memmi H. and Carbonell-Barrachina A. A. 2017. Deficit irrigation and emerging fruit crops as a strategy to save water in Mediterranean semiarid agrosystems. Agricultural Water Management. Agricultural Water Management, 202: 311-324.

Ghasemi M., Arzani K., Yadollahi A., and Hokmabadi H., 2013. Effect of drought stress on fluorescence, chlorophyll content and indices of four Pistachio Seedlings. Water Research in Agriculture, 27(4): 475-485. (In Persian).

Ghorchiani M., Etesami H., and Alikhani. H.A. 2018. Improvement of growth and yield of maize under water stress by co-inoculating an arbuscular mycorrhizal fungus and a plant growth promoting rhizobacterium together with phosphate fertilizers. Agriculture, Ecosystems and Environment. 258: 59-70.

Lotfi R., Gharavi-Kouchebagh P., and Khoshvaghti H. 2015. Biochemical and physiological responses of Brassica napus plants to humic acid under water stress. Russian Journal of Plant Physiology, 62(4): 480-486.

Madadi E., and Fallah S. 2017. Effects of jasmonic acid and humic acid to mitigate drought dtress Effect during pollination of forage Maize. Journal of Water and Soil, 31(5): 1396-1408. (In Persian)

Maleki Kuhbanani A. and Karimi H. 2013. An evaluation of the resistance of Pistachio rootstocks and one inter-spicific hybrid, P. atlantica × P. vera cv. ‘Badami- Riz- Zarand’ against drought Stress. Iranian Journal of Horticultural Science, 44(1): 81-93. (In Persian).

Munne-Bosch S., and Alegre L. 2004. Die and let live: leaf senescence contributes to plant survival under drought stress. Functional Plant Biology, 31: 203-216.

Orsi M. 2014. Molecular dynamics simulation of humic substances. Chemical and Biological Technologies in Agriculture, 1: 10.

Osuagwu G. G. E., Edeoga H. O., and Osuagwu A. N. 2010. The influence of water stress (drought) on the mineral and vitamin potential of the leaves Ocimum gratissimum L. Recent Research in Science and Technology, 2: 27-33.

Ozdamar Unlu H., Unlu H., Karakurt Y., and Padem, H. 2011. Changes in fruit yield and quality in response to foliar and soil humic acid application in cucumber. Scientific Research and Essays, 6: 2800-2803.

Ozfidan-Konakci C., Yildiztugay E., Bahtiyar M., and Kucukoduk M. 2018. The humic acid-induced changes in the water status, chlorophyll fluorescence and antioxidant defense systems of wheat leaves with cadmium stress. Ecotoxicology and Environmental Safety, 155: 66–75.

Pizzeghello D., Francioso O., Ertani A., Muscolo A., and Nardi, S., 2013. Isopentenyladenosine and cytokinin-like activity of different humic substances. Journal of Geochemical Exploration, 129: 70–75.

Salehi A., Tasdighi H., Gholamhoseini M. 2016. Evaluation of proline, chlorophyll, soluble sugar content and uptake of nutrients in the German chamomile (Matricaria chamomilla L.) under drought stress and organic fertilizer treatments. Asian Pacific Journal of Tropical Biomedicine, 6(10): 886–891.

Sarcheshmehpour M., Savaghebi G. R., Siadat H., Alikhani H. A. 2013. Effect of plant growth promoting rhizobacteria on improvement of nutrition and growth of Pistachio Seedlings under drought Stress. Iranian Journal of Soil Research, 27(1): 107-119. (In Persian)

Shamshiri M., Hasani M., Karimi H., and Esmaail Zadeh M. 2015. Effect of arbuscular mycorrhizae and salicylic acid on nutrient elements content of abareqi pistachio seedling under drought stress. Journal of Plant Productions, 38(1): 75-89.

Shool A., Shamshiri M., Akhgar A., Esmaeilizadeh M. 2014. Effect of arbuscular mycorrhizal fungi and Pseudomonas fluorescence on vegetative growth of pistachio seedlings (Pistacia vera cv. Qazvini) under four different irrigation regimes. Iranian Journal of Horticultural Science, 45(3): 297-307. (In Persian).

Turan M.A, Asik B.B., Katkat A.V., and Celik H. 2011. The effects of soil-applied humic substances to the dry weight and mineral nutrient uptake of maize plants under soil salinity conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39: 171-177.