نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری شیمی و حاصلخیزی خاک، دانشکده کشاورزی، دانشگاه زنجان

2 استاد گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

3 استادیارگروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه زنجان

4 استادیار پژوهشی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان همدان، سازمان تحقیقات، آموزش و ترویج کشاورزی

چکیده

استفاده تلفیقی از کلات­های شیمیایی در کنار کاشت گیاهان بیش انباشتگر،  به عنوان یک روش مؤثر در افزایش کارایی استخراج فلزات سنگین و کمک به اصلاح خاک­های آلوده، متداول است. وتیور به عنوان یک گیاه مقاوم به تنش شوری، خشکی و غلظت بالای فلزات سنگین شناخته شده است. اخیراً استفاده از کلات­های زیست­تخریب­پذیر در پالایش خاک از فلزات سنگین مد نظر پژوهشگران قرار گرفته است. پژوهش حاضر با هدف بررسی تأثیر سطوح مختلف کلات‌های زیست­تخریب­پذیر اتیلن دی آمین دی سوکسینیک اسید (EDDS)  و متیل گلایسین دی استیک اسید (MGDA) در چهار سطح (0، 1، 2و 4 میلی­مول در کیلوگرم خاک) بر افزایش میزان استخراج کروم 6 ظرفیتی در چهار سطح آلودگی خاک (.، 100، 200 و 400میلی­گرم در کیلوگرم خاک) توسط گونه وتیور (Chyrsopogon zizanioides L.)  انجام شد. استفاده از کلات MGDA با غلظت 4 میلی­مول در غلظت­های 0، 100، 200 و 400  میلی­گرم در کیلوگرم، به ترتیب نسبت جذب کروم ریشه را 62/1، 0/2، 48/1 و 5/2 برابر و در کلات EDDS به­ترتیب 5/1، 7/2، 25/2 و 9/2 برابر نسبت به شاهد افزایش داد. همچنین، استفاده از کلات MGDA با غلظت 4 میلی­مول، به­ترتیب نسبت کروم شاخسار را  08/3، 73/3، 23/3 و 33/2 برابر و در کلات EDDS، به ترتیب 52/2، 97/4، 49/3 و 5/5  برابر نسبت به شاهد افزایش داد. اثر سطوح غلظت کروم خاک و همچنین غلظت کلات­ها بر زیست­توده و خصوصیات رویشی ریشه و شاخسار کاهشی بود، اما کلات  EDDSموجب افزایش قطر بوته شد. جمع­بندی نتایج حاصل از بررسی شاخصه­های زیستی BCF و TF نشان داد گیاه وتیور توانمندی خاصی در پالایش خاک­های آلوده به کروم 6 ظرفیتی دارد و کلات EDDS با غلظت 4 میلی­مول در کیلوگرم خاک، سبب تشدید جذب کروم به خصوص در ریشه و افزایش انتقال آن به شاخسار گیاه وتیور می­شود. لذا، این ترکیب به عنوان یک کلات زیست­تخریب­پذیر مناسب در افزایش بازده تثبیت زیستی وتیور در خاک­های آلوده به کرومات معرفی می­شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Biodegradable Chelates MGDA and EDDS on Phytoextraction of Chromium byVetiver Grass in Chromate Contaminated Soils

نویسندگان [English]

  • mahdi Ahmadian 1
  • Ahmad Golchin 2
  • Parisa Alamdari 3
  • Ghasem Asadian 4

1 PhD candidate, Department of Soil Science, College of Agriculture, Zanjan University, Iran

2 Professor, Department of Soil Science, Faculty of Agriculture, Zanjan University, Zanjan. Iran

3 Professor, Department of Soil Science, Faculty of Agriculture, Zanjan University, Zanjan, Iran

4 Professor Researh, Hamedan agriculture and natural resources center, Areeo., Hamedan, Iran

چکیده [English]

The combined use of chemical chelates along with the planting of hyperaccumulator plants has been suggested as an effective method to increasing the extraction efficiency of heavy metals and helping to improve contaminated soils. Vetiver is known as a resistant plant to salinity, drought and high concentrations of heavy metals. Recently, the use of biodegradable chelates in soil refining from heavy metals has been considered by researchers. This study was designed to investigate the effect of different levels of biodegradable chelates Ethylenediamine-N,N'-disuccinic acid (EDDS) and methylglycinediacetic acid (MGDA) on increasing the adsorption of Cr(IV) by Vetiver grass. Chelates concentration were defined at four levels (0, 1, 2, 4 mmol/Kg soil) and soil chromium contamination at four levels of potassium dichromate (0, 100, 200, 400 ppm). The results showed that the vegetative growth characteristic decreased with increasing soil chromium and chelates concentration. The use of 4 Mmol of MGDA on soil chromium concentrations (0, 100, 200 and 400 ppm) increased the chromium ratio in root by 1.26, 2.0, 1.48 and 2.5 times and this ratio in treatments of EDDS were 1.5, 2.7, 2.25 and 2.9 times more than the control treatment, respectively. Also, the use of 4 Mmol of MGDA in shoots increased the chromium ratio by 2.8, 3.7, 4.1 and 4.2 times and in EDDS were 1.5, 3.8, 4.1 and 6.7 times more than the control. Increasing chelates concentration from 1 to 4 Mmol in all soil chromium levels increased the ratio of shoo/root chromium and showed higher performance of EDDS than MGDA. The effect of soil chromium levels and chelate concentrations on biomass and vegetative characteristics of roots and shoots was decreasing, but EDDS increased plant diameter. The results of bioconcetration factor (BCF) and translocation factor (TF) showed that Vetiver have a special ability in phytoextraction of soils contaminated with hexavalent chromium. The treatment of EDDS at 4 Mmol intensifies the absorption of chromium, especially in the roots and to some extent increases its transfer to the shoot of vetiver grass. Therefore, this compound is introduced as a suitable biodegradable chelate to increase phytoremediation efficiency by vetiver in chromate-contaminated soils.

کلیدواژه‌ها [English]

  • soil contamination
  • Chromium bioaccumulation
  • (Chyrsopogon zizanioides)
  • MGDA
  • EDDS
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