تاثیر زمان و ترکیب محیط کشت بر تولید IAA توسط جدایه های مختلف سودوموناس فلورسنت و تاثیر جدایه ها بر رشد ذرت (Zea mays L)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد گروه علوم خاک دانشگاه ولی‌عصر رفسنجان

2 استادیار گروه علوم خاک دانشگاه ولی‌عصر رفسنجان (مکاتبه کننده)

3 دانشیار گروه علوم خاک دانشگاه ولی‌عصر رفسنجان

4 استادیار گروه علوم و مهندسی خاک دانشکده فناوری کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی

چکیده

باکتری­های سودوموناس فلورسنت از مهم­ترین باکتری­های محرک رشد گیاه در ریزوسفر گیاهان مختلف زراعی می­باشند که با داشتن خصوصیات محرک رشدی متعدد، می­توانند موجب بهبود رشد گیاه گردند. تولید غلظت­های بالای IAA یکی از ویژگی‌های بارز برای اکثر این باکتری­ها است. به­منظور بررسی توانایی تولید اکسین توسط سویه­های مختلف باکتری سودوموناس فلورسنت در زمان­های متفاوت و در محیط­های کشت­ TSB و DF و همچنین تعیین تاثیرات این سویه­ها بر شاخص­های رشد گیاه ذرت، آزمایشی در قالب طرح کاملا تصادفی اجرا گردید. نتایج نشان­دهنده توانایی تمام سویه­ها، در تولید IAA بود. متوسط میزان تولید IAA در محیط کشت TSB بین 040/0 تا 3/34 میلی­گرم بر لیتر متغیر بود. سویه­های13p و 26p در روز پنجم به­ترتیب کم­ترین و بیشترین میزان IAA را تولید نمودند. در محیط کشت DF نیز متوسط تولید IAA توسط جدایه­ها بین 043/0 تا 03/6 میلی­گرم بر لیتر متغیر بود. همبستگی معنی­دار بین اکسین تولید شده در دو محیط کشت در روزهای مختلف، نشان داد که روند تولید اکسین توسط جدایه­ها در هر دو محیط کشت یکسان است و جدایه­هایی که توان بالایی در تولید اکسین دارند در هر دو محیط مقادیر بالاتری اکسین در مقایسه با جدایه­های ضعیف تولید کردند. نتایج آزمون گلخانه­ای نیز نشان داد که تلقیح جدایه­های مورد استفاده، وزن خشک اندام هوایی را تا بیش از 100 درصد و طول اندام هوایی، سطح برگ و کلروفیل را به­ترتیب 1/53، 2/57، 3/22 درصد افزایش دادند. کاربرد اکثر جدایه­ها سبب کاهش وزن خشک ریشه گردید. نتایج نشان­دهنده­ی وجود همبستگی مثبت و معنی­دار بین تولید IAA در دو محیط غنی TSB و حداقل DF بود. این همبستگی بیان­گر این است که در انتخاب جدایه­های برتر می­توان از هر دو محیط کشت استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of time and media composition on IAA production by different fluorescent Pseudomonads isolates and impact of isolates on the growth of maize (Zea mays L)

نویسندگان [English]

  • Dina Alsadat Rezayi 1
  • Payman Abbaszadeh-Dahaji 2
  • Abdolreza Akhgar 3
  • Ali Ashraf Soltani 4
1 1- M.Sc Student, Department of Soil Science, Vali-e-Asr University
2 Assistant professor, Department of Soil Science, Vali-e-Asr University
3 Associate professor Department of Soil Science, Vali-e-Asr University
4 Assistant Professor, Department of Soil Science, Faculty of Agricultural Technology and Natural Resources, University of Mohaghegh Ardabili
چکیده [English]

Fluorescent Pseudomonads are one of the most important plant growth promoting bacteria which exists in the rhizosphere of different crops, having different growth stimulating properties that can improve plant growth. Production of high concentrations of IAA is one of the typical characteristics for most of these bacteria. In order to evaluate the ability of auxin production by various strains of Pseudomonas fluorescens in different time period (in TSB and DF medium) and also to determine the effects of these strains on the growth of maize, a greenhouse experiment was done in a completely randomized design. The results showed that all isolates were able to produce IAA. The average amount of IAA in TSB medium ranged between 0.04 to 34.3 mg.l-1. The lowest and the highest IAA produced on fifth day by p13 and p26 isolates respectively. IAA production by the isolates in DF medium varid between 0.043 to 6.03 mg.l-1. Significant correlation between auxin production in both medium in different days showed that manner of auxin production by isolates were the same and isolates that have a high potential to produce auxin in both medium, produced higher amounts of auxin compared to inefficient isolates. The results of greenhouse experiment showed that the isolates inoculation increased shoot dry weight up to 100 percent while shoot length, leaf area and chlorophyll increased by 53.1, 57.2 and 22.3 percent respectively. Root dry weight was decreased by inoculation of most isolates. The results showed a significant positive correlation between the productions of IAA in both rich TSB and minimal DF medium. This correlation suggests that both media can be used for selection of superior strains.

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