بررسی واکنش لاین های خویش آمیخته نوترکیب آفتابگردان به تنش کلرید سدیم در مرحله گیاهچه ای

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد اصلاح نباتات، گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی دانشگاه ارومیه

2 دانشیار گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی دانشگاه ارومیه (مکاتبه کننده)

3 استادیار گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه مراغه

4 استادیار گروه علوم خاک دانشکده کشاورزی دانشگاه ارومیه

5 استادیار گروه زیست شناسی، دانشکده علوم دانشگاه ایلام

چکیده

به­منظور بررسی واکنش لاین­های خویش آمیخته نوترکیب آفتابگردان به تنش شوری در مرحله گیاهچه­ای، آزمایشی به­صورت فاکتوریل بر پایه طرح بلوک­های کامل تصادفی در شرایط کنترل شده اجرا گردید. فاکتور اول شامل 77 لاین خویش آمیخته نوترکیب (نسل F9) به همراه والدین و فاکتور دوم تنش شوری NaCl با سطوح شوری صفر و 6 (دسی­زیمنس­بر­متر) و صفات مورد مطالعه شامل طول ریشه­چه و ساقه­چه، وزن تر ریشه­چه و ساقه­چه و وزن خشک ریشه­چه و ساقه­چه بود. نتایج تجزیه واریانس نشان داد تمامی صفات مورد مطالعه تحت تأثیر ژنوتیپ، شوری و اثر متقابل ژنوتیپ و شوری قرار گرفتند. از مجموع 77 لاین، 43% (33 لاین) در سطح شوری 6 دسی­زیمنس­بر­متر قادر به جوانه زنی نبودند و 57% (44 لاین) قدرت زنده­مانی خود را حفظ کردند. میانگین تمام صفات مورد مطالعه در سطح شوری 6 دسی­زیمنس بر­متر در مقایسه با شرایط عادی کاهش نشان داد. خوشه­بندی لاین­های مورد مطالعه با در نظر گرفتن جمیع صفات در هر یک از دو شرایط تنش شوری و عادی با محاسبه فواصل مربع اقلیدسی (Squard Euclidean) و روش Ward انجام شد. لاین­ها در 3 خوشه گروه­بندی شدند. در شرایط بدون تنش در خوشه اول، دوم و سوم به ترتیب 17، 18 و 42 لاین و در شرایط تنش شوری به ترتیب 26، 8 و 9 لاین در خوشه­های مربوطه قرار گرفتند. بیشترین فاصله بین خوشه­های 1 با 2 مشاهده شد. نتایج تجزیه خوشه­ای در تطابق با نتایج حاصل از تجزیه به مؤلفه­های اصلی و تجزیه تابع تشخیص بود. لاین C86 برای اکثریت صفات مورد بررسی دارای بیشترین میانگین بود و به عنوان متحمل­ترین لاین به تنش شوری معرفی می­شود. 

کلیدواژه‌ها


عنوان مقاله [English]

Investigation on the reaction of sunflower recombinant inbred lines to NaCl stress at seedling stage

نویسندگان [English]

  • Fariba Morsali Aghajari 1
  • Reza Darvishzadeh 2
  • Hamid Hatami Maleki 3
  • Mohsen Barin 4
  • Ali Asghar Hatamnia 5
1 MSc Student, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Urmia, Urmia, Iran
3 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
4 Assistant Professor, Department of Soil Scinece, Faculty of Agriculture, Urmia University, Urmia, Iran.
5 Assistant Professor, Department of Biology, Faculty of Science, Ilam University, Ilam, Iran.
چکیده [English]

In order to investigate the reaction of sunflower recombinant inbred lines to salt stress in seedling stage, a factorial experiment based on randomized complete block design was implemented in controlled condition. The first factor consisted of 77 recombinant inbred lines (generation F9) and the second factor was salinity stress with NaCl (salinity levels 0 and 6 dS m-1) and traits including shoot length, root length, shoot fresh weight, root fresh weight, shoot dry weight and root dry weight were measured. Results of analysis of variance revealed that all studied traits influenced by genotype, salinity and genotype×salinity interaction. 33 lines out of 77 lines (43%) in salinity level of 6 dS m-1 could not germinate and 44 lines out of 77 lines (57%) could maintain their viability. Mean values of all of studied traits were decreased in salinity level of 6 dS m-1 compared to normal conditions. Clustering of genotypes was performed using the studied traits in each condition by squared Euclidean distance criteria and Ward algorithm. Studied lines were grouped in three classes. In normal conditions, 17, 18 and 42 lines were located in cluster one, two and three, respectively, while, in salinity conditions, 26, 8, and 9 lines were classified in above mentioned groups. Maximum distance was observed between cluster 1 and cluster 2. Results of cluster analysis were in coincidence with those obtained by principle component analysis and discriminant function analysis. Line C86 was possessed maximum values of studied traits and hence, present as salinity tolerant line.

کلیدواژه‌ها [English]

  • Cluster analysis
  • Salt stress
  • Seedling growth
  • Sunflower
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