اثر باکتری‌های ریزوسفری محرک رشد گیاه و سیدروفور بر توزیع شکل‌های شیمیایی روی در خاک و جذب آن توسط ذرت

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم خاک دانشگاه ولی عصر رفسنجان

2 دانشیار گروه علوم خاک دانشگاه ولی عصر رفسنجان

3 استادیار گروه علوم خاک دانشگاه ولی عصر رفسنجان

4 استاد گروه علوم باغبانی دانشگاه ولی عصر رفسنجان

چکیده

موفقیت در امر گیاه پالایی خاک­های آلوده به فلزات سنگین تا حد زیادی به فراهمی زیستی آن­ها بستگی دارد. برای بررسی برهمکنش سیدروفور DFOB (0، 70 و 140 میکرو مول دسفرواکسامین بر کیلوگرم خاک) و جدایه­های مقاوم به فلزات باکتری سودوموناس (p19, p18, p15, p0) بر تجمع روی در ذرت و تغییرات گونه­بندی شیمیایی آن، نمونه­هایی از یک خاک آلوده به روی به گلدان­های یک کیلوگرمی منتقل شد. گیاهان بعد از 60 روز برداشت شدند. نتایج نشان داد تلقیح باکتریایی گیاهان، غلظت روی در اندام هوایی گیاه را افزایش داد. هم­چنین اضافه نمودن 140 میکرومول سیدروفور بر گیلوگرم خاک به گیاهان تلقیح شده با جدایه p15، غلظت روی در ریشه گیاه را در مقایسه با شاهد به طور معنی‌داری افزایش داد. جذب روی نیز در گیاهان تلقیح شده با جدایه­های باکتریایی به طور معنی‌داری افزایش یافت. با کاربرد سیدروفور غلظت روی قابل استخراج توسط DTPA در خاک افزایش یافت. کاربرد سیدروفور و تلقیح جدایه­های باکتری، غلظت روی پیوند شده با کربنات­ها را به­طور معنی‌داری کاهش و غلظت روی محلول را افزایش داد. براساس نتایج این پژوهش، کاربرد لیگاند سیدروفور به همراه جدایه­های مقاوم باکتریایی می­تواند فراهمی زیستی روی را در خاک افزایش دهد که این موضوع از اهمیت زیادی در گیاه پالایی خاک­های آلوده برخوردار است.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of plant growth promoting rhizobacteria and siderophore on distribution of chemical fractions of Zn in soil and its uptake by corn

نویسندگان [English]

  • Hamideh Nemati 1
  • Mohsen hamid pur 2
  • Peyman Abbaszadeh 3
  • Hamid Reza Rosta 4
1 M.Sc. Student, Department of Soil Science, University of Vali Asr Rafsanjan
2 Associate Professor, Department of Soil Science, University of Vali Asr Rafsanjan
3 Associate Professor, Department of Soil Science, University of Vali Asr Rafsanjan
4 Professor, Department of Horticulture, University of Vali Asr Rafsanjan
چکیده [English]

Successful phytoremediation of metal-contaminated soils largely depends on their bioavailability in soil. Samples of a contaminated soil with elevated concentration of Zn were applied in the pots to evaluate the interactive effects of DFOB siderophore (0, 70 and 140 μM kg−1) and metal tolerant bacterial inoculation (p0, p15, p18, and p19) on Zn accumulation in corn and metal speciation and sequential fractions in soil. Results indicated that bacterial inoculation increased Zn concentrations in corn shoot. Addition of DFOB (140 μM kg−1) to pots of the soil which was inoculated with p15 isolate significantly enhanced Zn concentration in roots as compared to control. The uptake of Zn by shoots was increased in pots inoculated by bacterial isolates compared to those in the un-amended soils. Ligand addition significantly enhanced the concentration of DTPA-extractable Zn. Also co-application of DFOB and bacterial inoculation significantly decreased Zn bound to carbonates fraction and increased water soluble Zn concentration. Based on the results of this study, it can be concluded that co-application of DFOB and metal tolerant bacteria are efficient in increasing the bioavailability of Zn when expressed relative to the control treatment, which might be of great significance for the successful phyto-extraction of Zn-polluted soils.

کلیدواژه‌ها [English]

  • Soil pollution
  • Heavy metals
  • phytoremediation
  • Siderophore
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