تاثیر جدایه‌های محرک رشد گیاهی و مقاوم به آرسنیک بر خصوصیات فیزیولوژیک و غلظت عناصر غذایی پرمصرف گیاه پونه در یک خاک آلوده به آرسنیک

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشگاه محقق اردبیلی

2 دانش آموخته

3 هیات علمی

چکیده

به‌منظورانجام این تحقیق، یک آزمایش گلخانه‌ای به صورت طرح کاملا تصادفی در سه تکرار با استفاده از یک خاک آلوده به آرسنیک انجام شد. تیمار‌های آزمایش شامل مایه‌زنی گیاه پونه با 5 جدایه برتر از نظر برخی صفات محرک رشد گیاهی و مقاوم به آرسنیک و عدم مصرف مایه تلقیح بود. نتایج نشان داد که اثر مایه‌زنی پونه با جدایه‌های برتر بر مقدار پرولین در این گیاه معنی‌داری شد. بیشترین میزان تجمع پرولین (µg g-1FW 23/0) در گیاهان مایه‌زنی شده با جدایه‌ی AHG-6 مشاهده گردید. کاربرد جدایه‌های محرک رشد بر میزان مقادیر کلروفیل a، b، کلروفیل کل، شاخص کلروفیل و کارتنوئیدها تاثیر معنی‌داری داشت و باعث افزایش چشمگیر این شاخص‌ها در گیاهان مایه‌زنی شده در مقایسه با شاهد گردید. مایه‌زنی گیاه پونه با جدایه‌های محرک رشد تاثیر چندانی در غلظت فسفر گیاه در مقایسه با شاهد نداشت و اختلاف معنی‌داری بین آنها مشاهده نشد. کاربرد تمام ریزموجودات محرک رشد باعث افزایش غلظت منیزیم در اندام هوایی گیاه پونه در مقایسه با شاهد شد. این در حال بود که فقط جدایه‌های AHG-5 و AHG-6 مورد مطالعه باعث افزایش معنی‌دار منیزیم ریشه گیاه پونه در مقایسه با شاهد گردیدند. در مورد کلسیم و پتاسیم استفاده از جدایه‌های محرک رشد توانست باعث افزایش غلظت این عناصر در ریشه و اندام هوایی گیاه پونه گردد، اما اختلاف معنی‌داری بین شاهد و برخی از تیمارها وجود نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of plant growth promoting (PGP) and arsenic-resistant isolates on Physiological characteristics and macronutrients concentration of Mentha peparata L. in a arsenic-polluted soil

چکیده [English]

In order to perform this research, a greenhouse experiment was conducted in a completely randomized design with three replications. Treatments were involved: inoculation of Mentha peparata L. with 5 plant growth promoting (PGP) and arsenic-resistant isolates and control (without use of inoculant). Results revealed that inoculation of Oregano with superior isolates had significant effect on proline concentration. The greatest accumulations of proline (0.23 µg g-1FW) was observed in plants inoculated with AHG-6 isolate. The application of PGP isolates significantly increased chlorophylla, b, total chlorophyll content, chlorophyll index and carotenoids proportions compared with control. The inoculation of Mentha peparata L with PGP isolates has no significant effect on the plant phosphorus concentration. The utilization of all PGP microorganisms notably propagated the shoot magnesium concentration compared with control. Whereas only AHG-5 and AHG-6 significantly increased the root magnesium concentration. Regarding calcium and potassium, the use of PGP isolates augmented their concentration in the root and shoot, although there was no significant between some treatments and control
The utilization of all PGP microorganisms notably propagated the shoot magnesium concentration compared with control. Whereas only AHG-5 and AHG-6 significantly increased the root magnesium concentration. Regarding calcium and potassium, the use of PGP isolates augmented their concentration in the root and shoot, although there was no significant between some treatments and control.

کلیدواژه‌ها [English]

  • Mentha peparata L
  • growth promoting (PGP) and arsenic-resistant isolates
  • Physiological characteristics
  • nutrients
Ali B.M., Vajpayee P., Tripathi R.D., Rai U.N., Singht S.N., and Singhgh S.P. 2003. Phytoremediation of lead, nickel, and copper by Salix acmophylla boiss: Role of antioxidant enzymes and antixidant substances. Contamination and Toxicology, 70: 462-469.

Baker S., Barrentine W.L., Bowman D.H., Hoawthrone W.L., and Pettiet J.V. 1976. Crop response and arsenic uptake following soil incorporation of MSMA. Weed Science, 24: 322-326.

Baroni F., Boscagli A., Protano G., and Riccobono F. 2000. Antimony accumulation in Achillea ageratum, Plantago lanceolata and Silene vulgaris growing in an old Sb-mining area. Environmental Pollution, 109: 347- 352.

Bates I., Waldern R., and Tear I. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207.

Belimov A.A., Hontzeas N., Safronova V. I., Demchinskaya S. V., Piluzza G., Bullitta S., and Glick B. R. 2005. Cadmium- tolerant plant growth- promoting bacteria associated with the roots of India mustard (Brassica juncea L.). Soil Biology and Biochemistry, 37(2): 241-250.

Bremner J. M., and Mulvaney. C. S. 1982. Total nitrogen. In: Page A.L., Miller R.H., and Keeney D.R (Eds.), Methods of Soil Analysis. Part 2, American Society of Agronomy and Soil Science Society of America, Madison, WI, pp. 595-624.

Cempel M., and Nikel G. 2006. Nickel: a review of its sources and environmental toxicology. Polish Journal of Environmental Studies, 15: 375-382.

Chang J.S., and Kim I.S. 2010. Arsenic oxidation by Bacillus sp. strain Sea H-As22w isolated from coastal seawater in yeosu Bay. Environmental Engineering Research, 15: 15-21.

Cozzolino V., Pigna M., Di Meo V., Caporale A. G., Violante A., and Meharg, A. A. 2010. Influence of phosphate addition on the arsenic uptake by wheat (Triticum durum) grown in arsenic polluted soils. Fresenius Environmental Bulletin, 19 (5): 838-845.

Dabonne S., Koffi B., Kouadio E., Koffi A., Due E., and Kouame L. 2010. Traditional utensils: Potential sources of poisoning by heavy metals. British Journal of Pharmacology Toxicology, 1: 90-92.

Dary M. Chamber-Pérez M. A. Palomares A. J., and Pajuelo, E. 2010. in situ phytostabilisation of heavy metal polluted soils using Lupinus luteus inoculated with metal resistant plant-growth promoting rhizobacteria. Journal of Hazardous Materials, 177: 323-330.

Duel L. E., and Swoboda A. R. 1972. Arsenic Toxicity to cotton and soybeans. Journal of Environment Quality, 1: 317-320.

Eijsackers H. 2010. Earthworms as colonisers: Primary colonisation of contaminated land, and sediment andsoil waste deposits. Science of the Total Environment. 408: 1759-1769.

Gavrilescu M. 2004. Removal of heavy metals from the environment by biosorption. Engineering in Life Sciences, 3: 219-232.

Gee G. W., and Orr D. 2002. Particle- size analysis. Soil Science Society of America. Madison, 16: 255-293.

Geng C.N., Zhu Y.G., Liu W.J., and Smith S. E. 2005. Arsenate uptake and translocation in seedlings of two genotypes of rice is affected by external phosphate concentrations. Aquatic Botany, 83: 321-331.

Glick B. R. 1995. The enhancement of plant growth by- free- living bacteria. Canadian Journal of Microbiology, 41: 109-117.

Glick B. R. 2003. Phytoremediation: synergistic use of plants and bacteria to clean up the environment. Biotechnology Advances, 21: 383-393.

Gohre V., and Paszkowski U. 2006. Contribution of the arbuscular mycorrhizal symbiosis to heavy metal phytoremediation. Planta, 223: 1115-1122.

Grichko V. P., Fillby B., and Glick B. R. 2000. Increased ability of transgenic plants expressing the bacterial enzyme ACC deaminase to accumulate Cd, Co, Cu, Ni, Pb and Zn. Journal of Biotechnology, 81: 45-53.

Heidari M., Mousavinik S. M., and Golpayegani A. 2011. Plant growth promoting rhizobacteria (PGPR) effect on physiological parameters and mineral uptake in basil (Ociumum basilicm L.) under water stress. Journal of gricultural and Biological Science, 12: 6-11. (In Persian)

Jones J., and Benton. K. 2001. Laboratory guide for conducting soil tests and plant analysis. CRS Press, 308p.

Kazemalilou S., and Rasouli-Sadaghiani M.H. 2012. Effect of soil cadmium pollution on some physiological parameters of Hyoscyamus plant in presence/absence of growth-promoting microorganisms. Journal of Water and Soil Research, 22: 17-30. (In Persian)

Ladan S. 2009. Bioremediation of arsenic-polluted soils by Scallion & Ornamental cabbage. Tarbiat Modares University. PhD thesis. (In Persian)

Lichtenthaler H. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods of Enzymology, 148: 350-382.

Nagajyoti P.C., Dinakar N., Prasad T. N., Suresh C., and Damodharam. T. 2008. Heavy metal toxicity: Industrial effluent effect on groundnut (Arachis hypogaea L.) seedlings. Journal of Applied Sciences Research, 4: 110-121.

Olsen S.R., and Sommers L.E. 1982. Phosphorus.P403-427. In: Page A.L., Miller R.H., and Keeney D.R (Eds.), Methods of Soil Analysis. Part 2, American Society of Agronomy and Soil Science Society of America, Madison, WI.

Ordookhani K., Khavazi K., Moezzi A., and Rejali F. 2010. Influence of PGPR and AMF on antioxidant activity, lycopene and potassium contents in tomato. African Journal of Agricultural Research, 5(10): 1108-1116.

Pandey N., and Sharma C.P. 2002. Effect of heavy metals CO2, Ni2, and Cd2 on growth and metabolism of cabbage. Plant Science, 163:753-758.

Parida A. K., Dagaonkar V. S., Phalak M. S., and Aurangabadkar L. P. 2008. Differential response of the enzymes involved in proline biosynthesis and degradation in drought tolerant and sensitive cotton genotypes during drought stress and recovery. Acta Physiologiae Plantarum, 30: 619-627.

Pazoki A. R. 2012. Effect of lead, Azospirillum and humic acid on chlorophyll content, root and shootdry weight in rapeseed. Journal of Crop Production Research, 4 (2): 173-174.

Pigna M., Cozzolino V., Violanto A., and Meharg A. 2009. Influence of phosphate on the arsenic uptake by wheat (Triticum durum L.) irrigated with arsenic solutions at three different concentrations. Water, Air and Soil Pollution, 197: 371-380.

Pirdashti H., Tahmasebi – Sarvestani Z., and Bahmanyar M.A. 2009. Comparison of physiological response among four contrast rice cultivars under drought stress conditions. World Academy of Science, Engineering and Technology, 49: 52-53.

Rai V., Khatoon S., Bisht S.S., and Mehrotra S. 2005. Effect of cadmium ongrowth, ultramorphology of leaf and secondary metabolites of Phyllanthus amarus Schum and Thonn. Chemosphere, 61: 1644-1650.

Rajkumar M., and Freitas H. 2011. Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnology Advances, 29: 248-258.

Rajkumar M., Ma Y., and Freitas H. 2008. Characterization of metal resistant plant growth Bacillus weihenstephanensis isolated from serpentine soil in Portugal. Journal of Basic Microbiology, 48: 1-9.

 

Richards B. k., and Steenhuis T. S. 1998. Metal mobility at an old heavy metal loaded sludge application site. Environmental Pollution, 99: 365-377.

Srivastava S., Srivastava A.K., Suprasanna P., and D’Souza S.F. 2009. Comparative biochemical and transcriptional profiling of two contrasting varieties of Brassica juncea L. in response to arsenic exposure reveals mechanisms of stress perception and tolerance. Journal of Experimental Botany, 181: 1-13.

Stoeva N., and Bineva T. 2003. Oxidative Changes and photosynthesis in oat plants grown in As- contaminated Soil. Bulgarian Journal of Plant Physiology, 29(1-2):87-95.

Verma S., and Dubeym R.S. 2001. Effect of cadmium on soluble sugars and enzymes of their metabolism in rice. Biologia Plantarum, 1: 117-123.

Vitoria A.P., Cunhab M.D., and Azevedo R.A. 2006. Ultrastructural changes of radish leaf exposed to cadmium. Environmental and Experimental Botany, 58: 47-52.

Walkly A., and Black I.A. 1934. An examination of Degtijaref method for determining soil organic matter and a proposed modification of the chromic acid in soil analysis. I. Experimental. Soil Science Society of America Journal, 79: 459-465.

Wenzel W.W, Kirchbaumer N., and Prohaska T. 2001. Arsenic fractionation in soils using an improved sequential extraction procedure. Analytical Chimica Acta, 436: 309-323.