تأثیرEDTA ، اسید سیتریک و عصاره کود مرغی بر گیاه‌پالایی سرب با ذرت در یک خاک آلوده لومی شنی

نوع مقاله: مقاله پژوهشی

نویسندگان

دانشگاه شهرکرد

چکیده

از گیاهان می‌توان برای پاکسازی خاک از آلاینده‌ها استفاده کرد ولی، برای این امر ممکن است سالها و یا حتی چندین دهه وقت لازم باشد، به همین دلیل از تیمارهای شیمیایی و بیولوژیکی برای گیاه‌پالایی استفاده می‌شود. در پژوهش حاضر توانایی ذرت (هیبرید سینگل کراس 704) برای اندوزش سرب در یک خاک لومی شنی آلوده به سرب در قالب طرح کاملا تصادفی در سه تکرار در گلخانه بررسی شد. اسید سیتریک وEDTA در سطوح غلظتی صفر، 5/0 و 1 میلی‌مول بر کیلوگرم خاک و عصاره کود مرغی در سطوح غلظتی صفر، 5/0 و 1 گرم بر کیلوگرم خاک استفاده شدند. نتایج نشان داد که اثر کلات‌کننده‌ها بر غلظت سرب در اندام هوایی و ریشه، جذب سرب اندام هوایی و ضریب تغلیظ زیستی معنی‌دار (05/0p≤) بود. با افزایش غلظت کلات‌کننده‌ها وزن خشک اندام هوایی به‌صورت غیرمعنی‌دار کاهش یافت. استفاده از غلظت‌های 5/0 و 1 میلی‌مولEDTA بر کیلوگرم خاک غلظت سرب در اندام هوایی ذرت را به‌ترتیب 164 و 260 درصد برابر شاهد به‌صورت معنی‌دار افزایش داد. کاربرد 1 میلی‌مول بر کیلوگرم خاک اسید سیتریک و 1 گرم بر کیلوگرم خاک عصاره کود مرغی غلظت سرب را در اندام هوایی ذرت به‌ترتیب 122 و 116 درصد برابر شاهد افزایش داد. کاربرد 1میلی‌مول در کیلوگرم EDTA و اسید سیتریک ضریب تغلیظ زیستی را به‌ترتیب 145 و 140 درصد برابر شاهد به-صورت معنی‌دار افزایش داد. کاربرد غلظت 1 میلی‌مول EDTA در کیلوگرم خاک ضریب انتقال را 207 درصد برابر شاهد به‌صورت معنی‌دار افزایش داد. از آنجایی که استفاده زیاد از حد کلات‌کننده‌ها می‌تواند باعث قابلیت‌استفاده بیشتر سرب در خاک شود بدون این که جذب گیاه را افزایش دهد، لذا استفاده از سطوح غلظتی بالاتر توصیه نمی‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

The impact of EDTA, citric acid and poultry manure extract on phytoremediation of Pb by corn in a sandy loam contaminated soil

چکیده [English]

The plants which can be used to clean up the heavy metals contaminated soils but, for this reason may take years or even decades, because of this chemical and biological treatment uses for phytoremediation. In this study, the potential of corn (hybrid KSC. 704) for accumulation of lead was conducted as an experiment using a completely randomized design with three replications in a greenhouse experiment in a sandy loam soil contaminated with Pb. Citric acid and EDTA were used at concentrations level 0, 0.5 and 1 mmol kg-1 soil and poultry manure extract 0, 0.5 and 1 g kg-1 soil. The results showed that the effect of chelating agents on the shoots and roots Pb concentration, shoots absorption Pb and biological concentration factor (BCF) was (p <0.05) significant. With increasing chelators concentration shoots dry weight nonsignificantly decreased. The results showed that application of 0.5 and 1 mmol kg-1 soil concentrations of EDTA were significantly increased Pb concentration in corn shoots 164 and 260 (%) (significant for 1 mmol kg-1 of EDTA) times more than the control, respectively. Application of 1 mmol kg-1 soil citric acid and 1 g kg-1 soil poultry manure extract were increased Pb concentration in corn shoots 122 and 116 (%) times more than the control, respectively. Application of 1 mmol kg-1 soil EDTA and citric acid significantly increased bioconcentration factor 145 and 140 (%) times more than the control, respectively. Application of 1 mmol kg-1 soil EDTA significantly increased translocation factor 207 (%) times more than the control. Since inordinate use of chelators can make more Pb availibility in soil without increasing plant uptake, therefore application of more concentration level not recommend.

کلیدواژه‌ها [English]

  • phytoremediation
  • heavy metal
  • EDTA
  • citric acid
  • poultry manure extract
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