ارزیابی چند روش‌ عصاره‌گیری مس قابل جذب ذرت در برخی خاک‌های آهکی ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 عضو هیات علمی گروه علوم و مهندسی خاک دانشگاه تبریز

2 دانش آموخته کارشناسی ارشد

چکیده

در این پژوهش ارزیابی برخی روش‌های تعیین مس قابل‌جذب خاک برای محصول ذرت در 21 خاک آهکی با استفاده از نمونه‌های مرکب خاک سطحی (0-30 سانتی‌متر) انجام گرفت. طی آزمایش گلخانه‌ای گیاه ذرت (Zea mays L.) رقم سینگل کراس 704 در سه تکرار کشت شد. پس از 60 روز (پایان دوره رشد رویشی) شاخص سبزینه برگ قبل از برداشت و وزن تر و خشک بخش هوایی و مقدار مس جذب‌شده بخش هوایی گیاه بعد از برداشت اندازه­گیری شد. اکسالات آمونیوم، هیدروکسیل آمونیوم هیدروکلراید، DTPA-TEA، AB-DTPA، AC-EDTA، عصاره­گیرهای مورد بررسی در این پژوهش بودند. بر طبق نتایج حاصله مس عصاره­گیری شده توسط هیچ­یک از عصاره­گیرهای مورداستفاده در این پژوهش  با شاخص­های رشد ذرت همبستگی معنادار نداشت. لگاریتم مس عصاره­گیری شده با DTPA-TEA با لگاریتم مس جذب‌شده توسط ریشه ذرت ضریب همبستگی معنادار(p<0.05) نشان داد. عصاره‌گیرهای DTPA-TEA و اکسالات آمونیوم سریع به­ترتیب کمترین برابر با 57/1 و بیشترین مقدار مس برابر با 9/25 میلی‌گرم بر کیلوگرم را عصاره­گیری کردند. بیشترین ضریب همبستگی معنادار بین روش­های عصاره‌گیری AB-DTPA و AC-EDTA (**93/0=r) مشاهده شد. ضرایب همبستگی مس عصاره­گیری شده با DTPA-TEA و شاخص­های رشد ذرت در 48 ساعات اولیه عصاره­گیری مس روند افزایشی داشت و در این میان بیشترین ضریب همبستگی مثبت معنادار بین شاخص سبزینه برگ و مس عصاره­گیری شده با DTPA-TEA (*53/0=r) مشاهده شد. درنهایت عصاره­گیر DTPA-TEA به­عنوان عصاره­گیر مناسب در این پژوهش انتخاب شد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Some Extraction methods for Determination of Corn Available copper in Some Calcareous Soils of Iran

نویسنده [English]

  • Adel Reyhanitabar 1
چکیده [English]

The effectiveness of different extraction methods for the prediction of available Cu concentration was studied in 21 calcareous surface soils (0-30 cm) in western Iran. The greenhouse experiment was conducted with single cross 704 (SC-704) cultivar of corn plant (Zea mays L.) with three replications. The leaf chlorophyll index, shoot dry weight and shoot Cu concentration were measured at the end of the growing period (after 60 days). Various extractants were applied for extraction of soil available Cu, including DTPA, AB-DTPA, AC-EDTA, Hydroxylamine Hydrochloride, and Ammonium Oxalate. According to results, extracted Cu by various extractants was insignificantly correlated with plant growth indices. However, significantly correlation was found between The Log DTPA-TEA-Cu and Log Root-Cu content. The highest (25.9 mg kg-1) and the lowest (1.57 mg kg-1) concentrations of extractable Cu was extracted with ammonium oxalate and DTPA-TEA respectively. The most significant correlation coefficient (r=0.93**) was obtained between AB-DTPA and AC-EDTA extraction methods.  Results revealed that the DTPA-TEA was the most effective Cu extractant in this study.

کلیدواژه‌ها [English]

  • available Cu
  • Corn
  • correlation coefficient and extractant
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