جداسازی، شناسایی مولکولی و ارزیابی ویژگی‌های محرک رشدی باکتری‌های تشکیل دهنده بیوفیلم از ریزوسفر گیاهان غیرزراعی در شمال غرب ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه علوم و مهندسی خاک دانشگاه مراغه

2 گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز

3 علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز

4 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه مراغه

چکیده

باکتری­های ریزوسفری تشکیل دهنده بیوفیلم با ویژگی­های مطلوب محرک رشدی می­توانند تأثیر زیادی بر تولید گیاهان زراعی در شرایط تنش داشته باشند. بنابراین به‌منظور مطالعه این باکتری‌ها، 50 نمونه­ ریزوسفری از ریشه­ گیاهان وحشی گندمیان تهیه و پس از تهیه سوسپانسون در شرایط آزمایشگاهی روی محیط کشت TSA کشت و باکتری‌ها خالص‌سازی شدند. نتایج نشان داد که بیش از 90 درصد باکتری­های به‌دست آمده توانایی تشکیل بیوفیلم را دارا بوده و می‌توانند در پنج گروه روشناور، چسبندگی زیاد، چسبندگی متوسط، چسبندگی ضعیف و بدون قابلیت چسبیدن تقسیم­بندی شوند. ارزیابی تنوع ژنتیکی باکتری­های مؤثر جداسازی شده نشان داد که در شرایط این مطالعه به شش جنس مختلف باکتریایی تعلق دارند، اما بالغ بر 80 درصد آن‌ها از جنس باسیلوس بودند. نتایج مقاومت به خشکی روی 68 جدایه از 130 جدایه جداسازی شده با قدرت بالای تشکیل بیوفیلم با روش سوربیتول مشخص نمود که در پتانسیل ماتریک 25- بار تمامی جدایه­ها قادر به فعالیت و رشد بودند. از نظر ویژگی­های محرک رشدی تمامی جدایه­ها قادر به تولید هورمون اکسین در محدوده شش تا 65 میکروگرم بر میلی‌لیتر بوده، 96 درصد از جدایه‌ها قادر به تخریب 1-آمینو سیکلوپروپان کربوکسیلیک اسید و تولید آلفا کتو بوتیریک اسید تا 14/2 میکرومول در 36 ساعت بودند. توانایی انحلال فسفر بین 2/1 تا 251 میلی­گرم بر لیتر و قدرت آزادسازی پتاسیم از کانی مسکوویت در آن‌ها بین 90 تا 250 میلی­گرم بر لیتر متغیر بود. در بین باکتری­های مورد مطالعه فقط 41/4 درصد دارای توان تولید سیدروفور بودند. به‌نظر می­رسد که مجموعه باکتریایی خوبی در این مطالعه شناسایی شده­اند که می­توانند به‌عنوان محرک رشد گیاهان در آزمایش‌های گلخانه‌ای و گلدانی و سپس مزرعه­ای در شرایط تنش کم­آبی مورد استفاده قرار گیرند.

کلیدواژه‌ها


عنوان مقاله [English]

Isolation, Molecular Identification, and Assessing Plant Growth Promoting Activities of Biofilm Forming Bacteria from Gramineae Rhizosphere in North West of Iran

نویسندگان [English]

  • Esmaeil Karimi 1
  • Naser Aliasgharzad 2
  • Mohammad Reza Neyshabouri 3
  • Ezatallah Esfandiari Esfandiari 4
1 Soil Science Department, University of MAragheh
2 Soil Science department, faculty of agriculture, University of Tabriz
3 Soil science department, faculty of agriculture, university of Tabriz
4 agronomy department, faculty of agriculture, university of Margheh.
چکیده [English]

Plant-associated rhizospheric bacteria which form biofilms have higher positive effects on plants in fluctuating environments. Thus, to study of these bacteria, 50 rhizospheric samples were taken from grass geramineae roots. After preparing the suspension, 100 microlitere was cultured on TSA medium and then pure culture of bacteria was prepared. Resulted showed that up to 90% of these isolates were able to form biofilm, so that they could be categorized in five groups as: pellicle, very adherent, moderately adherent, weakly adherent and non-adherent. 16S rDNA analysis revealed six different genus of bacteria. Bacillus are the predominant group (80%) of robust biofilm forming in the grass geramineae rhizosphere. The water deficit tolerance of isolates was assessed by rising concentration of sorbitol (1, 10, 20 and 30 %, w/v). All isolates were able to grow in -25 bar matric potential. Isolates showed good potential to producing auxins ranging from 6 - 65 µg mL-1, ACC-deaminase activity (up to 2.14 µmol α-ketobutyrate in 36 h), Ca3(PO4)2 solublization ranging from 1.2 – 251 mg L-1 and K releasing ability 90 to 250 mg L-1. Only three bacteria were able to produce siderophore. It seems that these isolates can be suitable candidates for doing some greeanhaous or pot experiment subjected to protecting plants against water deficit stress. Moreover, they can act as plant growth promoting bacteria as well.

کلیدواژه‌ها [English]

  • 16S rDNA
  • ACC-deaminase
  • Bacillus
  • K releasing
  • Water deficit stress
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