نقش میکروب‌های خاک در پالایش‌سبز خاک آلوده به کادمیم توسط گیاه خار زن ‌بابا (Onopordon acanthium L.)

نوع مقاله: مقاله پژوهشی


گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه


مایه­زنی میکروبی، در بهبود رشد و افزایش تحمل گیاهان به تنش­های محیطی و پالایش سبز خاک­های آلوده به فلزات سنگین، موثرند. به منظور بررسی نقش برخی گونه­های قارچ­ریشه­های آربوسکولار (AMF) (ترکیبی از گونه­های GlomusشاملG. mosseae، G. intraradicesوG. fasciculatum)و باکتری­های محرک رشد گیاه (PGPR) (ترکیبی از گونه­های Pseudomonas  شامل برخی سویه­های P. fluorescens ، P.putidaوP. aeruginosa) در پالایش آلودگی کادمیم خاک توسط گیاه خارزن ­بابا (Onopordon acanthium L)، آزمایشی گلخانه ای به صورت فاکتوریل در قالب طرح پایه بلوک­های کامل تصادفی با 3 تکرار اجرا شد. فاکتور اول غلظت کادمیم در چهار سطح شامل صفر، 10، 30 و 100 میلی گرم کادمیم بر کیلوگرم و فاکتور دوم تیمار میکروبی در سه سطح شامل AMF، PGPR و شاهد بودند. یک نمونه خاک با نمک نیترات کادمیم به­طور یکنواخت برای ایجاد غلظت­های مختلف کادمیم (صفر، 10، 30 و 100 میلی گرم کادمیم بر کیلوگرم) آلوده شد. خاک آلوده شده استریل و سپس با AMF و PGPR مایه­زنی شد. نتایج نشان داد که با افزایش غلظت کادمیم در خاک، درصد کلنیزاسیون، فراوانی باکتری­های ریزوسفری، عملکرد و عملکرد نسبی شاخساره­ به طور معنی­داری (05/0P≤) کاهش یافت، اما مقدار پرولین، غلظت کادمیم در شاخساره به­طور معنی­داری (05/0P≤) افزایش یافت. میانگین کادمیم استخراج شده در تیمارهای PGPR و  AMF به­ترتیب 1/3 و 6/2 برابر بیش از تیمارهای شاهد بود. از نتایج بدست آمده در این آزمایش می­توان نتیجه گیری کرد که مایه زنی با AMF و PGPR می‌تواند روشی نوید بخش برای افزایش توان گیاه خارزن­بابا در استخراج کادمیم از خاک­های آلوده باشد.


عنوان مقاله [English]

Role of Soil Microbes in Phytoremediation of Cadmium Contaminated Soils by onopordon (Onopordon acanthium L.)

نویسنده [English]

  • Mohsen Barin
Department of Soil Science, Urmia University, Iran
چکیده [English]

Microbial inoculation improves plants growth and increases their tolerance to environmental stresses and induces phytoremediation of heavy metals contaminated soils. In order to evaluate the role of some strains of AMF (a mixture of Glomus speciesincluding G. intraradices, G. mosseae and G. fasciculatum) and PGPR (a mixture of Pseudomonas speciesincludeing P. putida, P. fluorescens, and P. aeruginosa) in reclamation of cadmium (Cd) contaminated soils by Onopordon (Onopordon acanthium L) a factorial experiment based on a randomized complete block design and in three replications was carried out in greenhouse condition. The first factor was Cd concentration in four levels including 0, 10, 30 and 100 mg kg-1 and the second factor was microbial treatment in three levels including non-inoculation, and AMF or PGPR inoculation. A soil sample was spiked uniformly with Cd nitrate salt to create different Cd concentrations. The contaminated soils were then sterilized and subsequently inoculated with AMF and PGPR. Results showed that with increasing soil Cd concentration, colonization percent, and abundance of rhizobacteria, shoot biomass and shoot relative biomass significantlydecreased, while proline content and the shoot Cd concentration significantly increased (P ≤ 0.05). Mean Cd extracted in AMF and PGPR treatments were respectively 3.1 and 2.6 order of magnitude higher compared to the corresponding blank treatments. Therefore, it could be concluded that inoculation with species of AMF and PGPR can be a promising technique for enhancing the potential of onopordon plant in extraction of Cd from contaminated soils.

کلیدواژه‌ها [English]

  • Phytoextraction
  • Growth promoting rhizobacteria
  • Heavy metals
  • Mycorrhiza

Abou-Shanab RA Angle JS and Ghaney RL. 2006. Bacterial inoculants affecting nickel uptake by Alyssum murale from low, moderate and high Ni soils. Soil Biology and Biochemistry,38: 2886-2889.

Arriagada CA, Herrera MA and Ocampo JA. 2005. Contribution of arbuscular mycorrhizal and saprobe fungi to the tolerance of Eucalyptus globulus to Pb. Water, Air and Soil Pollution, 166: 31-47.

Arshad M, Saleem M and Hussain S. 2007. Perspectives of bacterial ACC deaminase in phytoremediation. Trends in Biotechnology, 25: 356–362.

Awotoye OO, Adewole MB, Salami AO and Ohiembor MO. 2009. Arbuscular mycorrhiza contribution to the growth performance and heavy metal uptake of Helianthus annuus LINN in pot culture. African Journal Environment Science and Technology, 3: 157-163.

Bates L, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205-207.

Belimov AA, Sarfronova VI and Mimura T. 2002. Response of spring rape to inoculation with plant growth-promoting rhizobacteria containing 1-aminocyclipropane-1-carboxylate deaminase depends on nutrient status of the plant . Candian Journal of Microbiology, 48: 189-199.

Cariny T. 1995. The Reuse of Contaminated Land .John Wiley and Sons Ltd. Publisher. 219 p.

Carter MR and Gregorich EG. 2008. Soil sampling and methods of analysis (2nd ed). CRC Press Boca   Raton, Florida, 1204 p.

ChenSK, Edwards CA and Subler S. 2001. Effects of the fungicides benomyl, captan, chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biology and Biochemistry, 33: 1971-1980.

Clark RB and Zeto SK. 2000. Mineral acquisition by arbuscular mycorrhizal plants. Journal Plant Nutrition, 23: 867-902.

Dary M, Chamber-Perez MA, Palomares AJ and Pajuelo E. 2010. In situ phytostabilisation of heavy metal polluted soils using Lupinus luteus inoculated with metal resistant plant-growth promoting rhizobacteria. Journal of Hazardous Material, 177: 323-330.

Das P, Samantaray S, Routm GR. 1997. Studies on cadmium toxicity in plants: A review. Environmental Pollution, 98: 29–36.

Diaz G, Azcon-Aguilar C and Honrubia M. 1996. Influence of arbuscular mycorrihza on heavy metal (Zn and Pb) uptake and growth of Lygedum spartum and Anthyllis cytisoides. Plant and Soil, 180: 241-249.

Giovannetti, M, and B. Mosse. 1980. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist, 84: 489-500.

Hovsepyan A and Greipsson S. 2004. Effect of arbuscular mycorrhizal fungi on phytoextraction bycorn (Zea mays) of lead-contaminated soil. International Journal of Phytoremediation, 6: 305-321.

Joner EJ and Leyval C. 1997. Uptake of Cd by roots and hyphae of a Glomus mosseae/Trifolium subterraneum mycorrhiza from soil amended with high and low concentrations of cadmium. New Phytologist, 135: 353–360.

Kaldorf M, Kuhn AJ,. Schroder WH, Hildebrandt U and Bothe H. 1999. Selective element deposits in maize colonized by a heavy metal tolerance conferring arbuscular mycorrhizal fungus. Journal of Plant Physiology, 154: 718-728.

Kamnev AA and Lelie DV. 2000. Chemical and biological parameters as tools to evaluate and improve heavy metal phytoremediation. Bioscience Reports, 20: 239-258.

Karimi A, Khodaverdiloo H. and Rasoli-sadaghiani MH. 2013. Induction effect of some species of Glomus and Pseudomonas in phytoremediation of soil Pb by Hyoscyamus niger. Journal of Soil and Water Science, 23: 227-243. (In Persian).

Karimi A, Khodaverdiloo H and Rasouli-Sadaghiani MH. 2011. Effect of plant growth-promoting organisms on growth and yield 0f pasture plant (Onopordon acanthium) in a lead contaminated soil. 12th Iranian Soil Science Congress, 3-5 September 2011, Tabriz, Iran.

Kazemalilou S, Rasouli-Sadaghiani MH. 2012. Effect of soil cadmium pollution on some physiological parameters of Hyoscyamus plant in presence/absence of growth-promoting microorganisms. Water and Soil Sciences, 22: 17-30. (In Persian).

Kazemalilou S, Rasouli-Sadaghiani MH, Khodaverdiloo H and Barin M. 2013. Soil Cd contamination and evaluation of its effects on soil biological quality and plant growth. Applied Soil Research,  1: 24-40. (In Persian).

Khan AG. 2005. Mycorrhizas and phytoremediation. In: Willey N, (ed.). Method in biotechnology phytoremediation: Methods and reviews. Totowa, USA, Humana Press, 494p.

Khodaverdiloo H. 2006. Modeling phytoremediation soils polluted with cadmium and lead. PhD thesis. TarbiatModaresUniversity, Tehran, Iran. 131p. (In Persian).

Khodaverdiloo H, Rahmanian M, Rezapour S, Ghorbani Dashtaki Sh, Hadi H and Han FX. 2012. Effect of wetting-drying cycles on redistribution of lead in some semi-arid zone soils spiked with a lead salt. Pedosphere, 22: 304–313.

Khodaverdiloo H, Rasouli-Sadaghiani MH and Karimi A. 2013. Influence of microbial inoculation of a Pb-contaminated soil on growth, some physiological properties, and uptake and translocation of Pb, Fe, and Zn by Centaurea (Centaurea cyanus). Journal of Soil Management and Sustainable Production, 3: 75-93. (In Persian).

Khodaverdiloo HS, Ghorbani h, Dashtaki Sh and Rezapour S. 2011. Lead and cadmium accumulation potential and toxicity threshold determined for land cress (Barbarea verna) and spinach (Spinacia oleracea L.). International Journal of Plant Production, 5: 275-281.

Ma Y, Prasad MNV, Rajkumar M and Freitas H. 2011. Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnology Advances, 29: 248–258.

Malcova R, Rydlova J and Vosatka M. 2003. Metal-free cultivation of Glomus sp. BEG 140 isolated from Mn-contaminated soil reduces tolerance to Mn. Mycorrhiza, 13: 151-157.

Marschner H and Dell B, 1994. Nutrient uptake in mycorrhizal symbiosis. Plant and Soil, 159: 89-102.

Metwally A, Finkemeier I, George M, and Dietz KJ. 2003. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiology, 132: 272-281.

Munns R, Husain S, Rivelli AR, James RA, Condon AG, Lindsay MP, Lagudah ES, Schachtman DP and Hare RA. 2002. Avenues for increasing salt tolerance of crops and the role of physiologically based selection traits. Plant and Soil, 247: 93-105.

Oudeh M, Khan M and Scullion J. 2002. Plant accumulation of potentially toxic elements in sewage sludge as affected by soil organic matter level and mycorrhizal fungi. Environmental Pollution, 6: 293–300.

Punamiya P, Datta R, Sarkar D, Barber S, Patel M and Da P. 2010. Symbiotic role of Glomus mosseae in phytoextraction of lead in vetiver grass )Chrysopogon zizanioides L.). Journal of Hazardous Materials, 177: 465-474.

Rasouli-Sadaghiani MH, Khavazi K, Rahimian H, Malekoti MJ and Asadi Rahmani H. 2006. Evaluation of potential of native strains of Pseudomonas in wheat rhizosphere for sidrophore production. Journal of Water and Soil Science, 20:133-143. (In Persian).

Salt DE, Blaylock M, Kumar NP, Dushenkov V, Ensley BD, Chet I and RaskinI. 1995. Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants. Nature Biotechnology, 13: 468 - 474.

Sanita di, Toppi L and Gabbrielli, R. 1999. Response to cadmium in higher plants: A review. Environmental and Experimental Botany, 4: 105–130.

Sharifi Z, Safari Sinegani AA and Shariati S. 2012. Potential of indigenous plant species for the phytoremediation of arsenic contaminated land in Kurdistan (Iran). Soil and Sediment Contamination an International Journal, 21: 557-573.

Tutin TG, Heywood VH, Burges NA, Moore DM, Burges NA, Valentine DH, Walters SM and Webb DA. 1976. Flora Europaea, vol 4, Cambridge University Press, 629p.

Vassilev A and YordanovI. 1997. Reductive analysis of factors limiting growth of cadmium treated plants review. Plant Physiology, 23: 114-133.

Verma P, George KV and Singh HV. 2007. Modeling cadmium accumulation in radish, carrot, spinach and cabbage. Applied Mathematical Modeling, 31(8): 1652–1661.

Vessey JK. 2003. Plant growth promoting rhizobacteria as biofertilizer. Plant and Soil, 255: 271- 586.