ارزیابی چند روش‌ عصاره‌گیری مس قابل جذب ذرت در برخی خاک‌های آهکی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی گروه علوم و مهندسی خاک دانشگاه تبریز

2 دانش آموخته کارشناسی ارشد

چکیده

در این پژوهش ارزیابی برخی روش‌های تعیین مس قابل‌جذب خاک برای محصول ذرت در 21 خاک آهکی با استفاده از نمونه‌های مرکب خاک سطحی (0-30 سانتی‌متر) انجام گرفت. طی آزمایش گلخانه‌ای گیاه ذرت (Zea mays L.) رقم سینگل کراس 704 در سه تکرار کشت شد. پس از 60 روز (پایان دوره رشد رویشی) شاخص سبزینه برگ قبل از برداشت و وزن تر و خشک بخش هوایی و مقدار مس جذب‌شده بخش هوایی گیاه بعد از برداشت اندازه­گیری شد. اکسالات آمونیوم، هیدروکسیل آمونیوم هیدروکلراید، DTPA-TEA، AB-DTPA، AC-EDTA، عصاره­گیرهای مورد بررسی در این پژوهش بودند. بر طبق نتایج حاصله مس عصاره­گیری شده توسط هیچ­یک از عصاره­گیرهای مورداستفاده در این پژوهش  با شاخص­های رشد ذرت همبستگی معنادار نداشت. لگاریتم مس عصاره­گیری شده با DTPA-TEA با لگاریتم مس جذب‌شده توسط ریشه ذرت ضریب همبستگی معنادار(p<0.05) نشان داد. عصاره‌گیرهای DTPA-TEA و اکسالات آمونیوم سریع به­ترتیب کمترین برابر با 57/1 و بیشترین مقدار مس برابر با 9/25 میلی‌گرم بر کیلوگرم را عصاره­گیری کردند. بیشترین ضریب همبستگی معنادار بین روش­های عصاره‌گیری AB-DTPA و AC-EDTA (**93/0=r) مشاهده شد. ضرایب همبستگی مس عصاره­گیری شده با DTPA-TEA و شاخص­های رشد ذرت در 48 ساعات اولیه عصاره­گیری مس روند افزایشی داشت و در این میان بیشترین ضریب همبستگی مثبت معنادار بین شاخص سبزینه برگ و مس عصاره­گیری شده با DTPA-TEA (*53/0=r) مشاهده شد. درنهایت عصاره­گیر DTPA-TEA به­عنوان عصاره­گیر مناسب در این پژوهش انتخاب شد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Some Extraction methods for Determination of Corn Available copper in Some Calcareous Soils of Iran

نویسنده [English]

  • Adel Reyhanitabar 1
1
2
چکیده [English]

The effectiveness of different extraction methods for the prediction of available Cu concentration was studied in 21 calcareous surface soils (0-30 cm) in western Iran. The greenhouse experiment was conducted with single cross 704 (SC-704) cultivar of corn plant (Zea mays L.) with three replications. The leaf chlorophyll index, shoot dry weight and shoot Cu concentration were measured at the end of the growing period (after 60 days). Various extractants were applied for extraction of soil available Cu, including DTPA, AB-DTPA, AC-EDTA, Hydroxylamine Hydrochloride, and Ammonium Oxalate. According to results, extracted Cu by various extractants was insignificantly correlated with plant growth indices. However, significantly correlation was found between The Log DTPA-TEA-Cu and Log Root-Cu content. The highest (25.9 mg kg-1) and the lowest (1.57 mg kg-1) concentrations of extractable Cu was extracted with ammonium oxalate and DTPA-TEA respectively. The most significant correlation coefficient (r=0.93**) was obtained between AB-DTPA and AC-EDTA extraction methods.  Results revealed that the DTPA-TEA was the most effective Cu extractant in this study.

کلیدواژه‌ها [English]

  • Available Cu
  • corn
  • correlation coefficient and extractant
Agricultural Statistics. 2013. The first volume of agricultural crops, 2012. Ministry of Agricultural Jihad, Vice Chancellor for of Planning and Economic, Center of Information and Communication Technology. 164p. (In Persian)
Alva A.K., Hung B., and Paramasivam S. 2000. Soil pH affects copper fractionation and phytotoxicity. Soil Science Society America Journal, 64: 955-962.
Allison L.E., and Moodie C.D. 1965. In: Black C.A. (Ed), Methods of Soil Analysis, Part 2, Carbonate,American Society of Agronomy, Soil Science Society of America, Madison, WI, pp. 1379-1396.
Bawer C.A., Reitemeier R.F., and Fireman M. 1952. Exchangeable cation analysis of saline and alkali soil. Soil Science, 73: 251-261.
Benitez M.L., Pedraja V.M., Del Campillo M.C., and Torrent J. 2002. Iron chlorosis in olive in relation to soil properties. Nutrient Cycling Agroecosystem, 62: 47-52.
Bernhard W., and Sperling M. 2008. Atomic Absorption Spectrometry. John Wiley and Sons.
Black A., McLaren R.G., Reichman S.M., Speir T.W., and Condron M.L. 2011. Evaluation of soil metal bioavailability estimates using two plant species (perenne T. and aestivum L.) grown in a range of agricultural soils treated with biosolids and metal salts. Environmental Pollution, 159: 1523-1535.
Brun L.A., Maillet J., Hinsinger P., and Pepin M. 2001. Evaluation of copper availability to plants in copper-contaminated vineyard soils. Environmental Pollution, 111:  293-302.
Correa R.N., Wanderley J.M., and Ferracciu Alleoni L.R. 2013.Testing extractants for Cu, Fe, Mn and Zn in tropical soil treated with sewage sludge for 13 consecutive years. Water, Air, Soil Pollution, 5: 224-1557.
Del Campillo M.C., and Torrent J. 1992. A rapid acid-oxalate extraction procedure for the determination of active Fe-oxide forms in calcareous soils. Journal of Plant Nutrition and Soil Science, 155: 437-440.
De Santiago A., and Delgado A. 2006. Predicting iron chlorosis of lupin in calcareous Spanish soils from iron extracts. Soil Science Society of America Journal, 70(6): 1945-1950.
Drouineau G. 1942. Dosage rapide du calcaire actif du sol: Nouvelles donne es sur la se parathion et la nature des fractions calcaires. Analysis of Agronomy, 12: 441-450.
Fageria N.K., and Barbosa Filho V.C. 2006. Identification and correction nutrient deficiencies in rice. Embrapa Arroze Feijao Circular number, 7 p.
Feng M.H., Shan X.Q., Zhang S.Z., and Wen B. 2005. Comparison of a rhizosphere-based method with other onestep extraction methods for assessing the bioavailability of soil metals to wheat. Chemosphere, 59: 939-949.
Fonseca A.F., Cairess E.F., and Barth G. 2010. Extraction methods and availability of micronutrients for wheat under a no-till system with a surface application of lime. Science Agriculture, 67: 60-70.
Gee G.W., and Or, D. 2002. Particle size analysis. Methods of Soil Analysis. Part 4. Physical Methods. Soil Science Society of America, pp. 201-214.
Havlin J.L., and Soltanpour P.N. 1981. Evaluation of NH4HCO3-DTPA soil test for iron and zinc. Soil Science Society of America Journal, 45(1): 70-75.
Haynes R.J., and Swift R.S. 1983. An evaluation of the use of DTPA and EDTA as extractants for micronutrients in moderately acid soils. Plant and Soil, 74(1): 111-122.
Jones J.B. 2001. Laboratory guide for conducting soil tests and plant analysis. Cereals Research of Community Press, 384p.
Kamkar B., Langerodi V., and Mohammadi R. 2012. Application of minerals in feeding of crops. Mashhad Jihad University press. (In Persian)
 Kocialkowski W.Z., Diatta J.B., and Grzebisz W. 1999. Evaluation of chelating agents as heavy metals extractants in agricultural soils under threat of contamination. Polish Journal of Environmental Studies, 3: 149-154.
Lindsay W.L. 1979. Chemical Equilibria in Soils. John Wiley and Sons, New York.pp.449
Lindsay W.L., and Cox F. 1985. Micronutrient soil testing for the tropic. In: Paul L., and Velk G. (Ed). Micronutrients In Tropical Food Crop Production. Spring Netherland, pp. 169-200.
Lindsay W.L., and Norvell W.A. 1978. Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of America Journal, 42: 421-428.
Loeppert R.H., and Suarez D.L. 1996. Carbonate and Gypsum. Publications from USDA-Agricultural Research Service.  University of Nebraska-Lincoln, 504p.
Mafton M.V., Mohasseli N., Karimian and Ronaghi A.M. 2003. Laboratory and greenhouse evaluation of five chemical extractants for estimating available copper in selected calcareous soils of Iran. Communications in Soil Science and Plant Analysis, 34: 1451-1463.
Marchi G., Guilherme L.R.G., Lima J.M., Change A.C., and Fontes R.L. 2006. Adsorption/desorption of organic anions in Brazilian Oxisols. Communications in Soil Science and Plant Analysis, 37: 1367-1379.
Marschner H. 1985. Mineral nutrition of higher plants. (2nd Ed). Academic Press. New York, NY, pp. 70-82.
Menzies N.W., Donn M.J and Kopittke P.M. 2007. Evaluation of extractants for estimation of the phytoavailable trace metals in soils. Environmental Pollution, 145: 121-130.
Motaghian H.R., Hossainpour A.R., Raeisi F., and Mohammadi J. 2013. Assessement of several extractants for determination of copper bioavailability to whet (Triticum aestivum L.) in sewage sludge-treated calcareous soils. Ejgcst, 4(15): 51-65. (In Persian)
Motaghian H.R., and Hosain pour A.R. 2014. Assessement of several extractants for determination of copper bioavailability to bean (Phaseolus vulgaris L.) in sewage sludge-treated and non-sewage sludge-treated. Journal of Science and Technology of Greenhouse Culture, 17: 113-124. (In Persian)
Nelson D.W., and Sommers L.E. 1966. Total carbon, organic Chemical methods. In: Sparks D.L., Page A.L., Helmke P.A., Loppert R.H., Soltanpour P.N., Tabatabai M.A., Johanson G.T., and Summer M.E. (Ed). American Society of Agronomy. Madison, WI, pp. 961-1010.
Ponnamperuma F.N., Cayton M.T., and Latin R.S. 1981. Dilute hydrochloric acid as an extractant for available zinc, copper and boron in rice soil. Plant and Soil, 61: 297-310.
Richards L.K. 1954. Diagnosis and improvement of saline and alkaline soils. Agriculture Hand Book, Salinity laboratory Staffs. Department of Agriculture, pp. 69-72.
Rhoades J.D. 1996. Salinity: electrical conductivity and total dissolved solids. In: Sparks D.L. Methods of Soil Analysis, Part 3, Chemical Methods. Soil Science Society of America, Madison, WI, pp. 417-436.
Sakal R., Singh A.P., and Singh S.P. 1988. Distribution of available zinc, copper, iron and manganese in old alluvial soils as related to certain soil characteristics. Journal of Indian Society Soil Science, 36: 59-63.
Sandermann G., and Boger P. 1983. The enzymatological function of heavy metals and their role in the electron transfer processes of plants. In: Lauchi A., and Bicleski R.L. (Ed). Encyclopedia of Plant Physiology, New Serve Vol 15. Berlin, pp. 563-596.
Sedberry J.E., Bligh D.P., and Eun M.Y. 1988. An evaluation of chemical methods for extracting copper from rice soils. Community of Soil Science and Plant Analysis, 19: 1841–1857.
Singh R.R and Prasad B. 1986. Selection of suitable extractant for predicting the response of barley (Hordeum Vulgar L.) to copper application in calcareous soils. Plant and Soil, 93: 211-222.
Sims J.T., and Johnson G.V. 1991. Micronutrient soil tests. In: Mortvedt J.J. (Ed). Micronutrients in Agriculture. Soil Science Society America. Madison, WI, pp. 427- 476.
Sharma C.P. 1992. 25 years of micro- and secondary nutreints research in soils and plants. Teach Bull. AICRP. Lucknow University.184p.
Shahbazi K, and Besharati H. 2014. Overview of agricultural soil fertility status of Iran. Journal of Land Management, 1 (1):1-17. (In Persian)
Shikanai T., Muller-Moule P., Munekage Y., Niyogi K.K., and Pilon M. 2003. PPA1, a P-type ATPase of Arabidopsis, functions in copper transport in chloroplasts. The Plant Cell, 15: 1333-1346.
Shorrocks V.M and Alloway B.J. 1988. Copper in plant, animal and human nutrition.Copper Development Association Publication, pp. 98-104.
Slavek J., Wold J., and Pickering W.F. 1982. Selective extraction of metal ions associated with humic acids. Talanta, 29(9): 743–749.
Soltanpour P.N., and Schwab A.P. 1977. A new soil test for simultaneous extraction of macro and micro-nutrients in alkaline soils. Communications in Soil Science and Plant Analysis, 8(3): 195-207.
Tabandeh L., Mafton M., and Karimian N. 2008. Comparition of different chemical extractants to determination the available copper of rice in calcareous soils of Fars province. Soil Research Journal of (soil and water science),22(2): 189-201. (In Persian)
Trier Weiler J.F and Lindsay W.L. 1969. EDTA-Ammonium carbonate soil test for zinc. Soil Science of America, 33: 49-53.
Wang J.J., Harrell D.L., Henderson and Bell P.F. 2004. Comparison of soil-test extractants for phosphorus, potassium, calcium, magnesium, sodium, zinc, copper, manganese, and Iron in Louisiana soils. Communication in Soil Science and Plant Analysis, 35(1 and 2): 145–160.
Williams C., Nascimento A., Eduardo E., Severina R., and Leite P .2007. Effect of liming on the plant availability and distribution of zinc and copper among soil fractions. Communications in Soil Science and Plant Analysis, 38: 545–560.