تأثیر کودهای آلی و شیمیایی بر میزان جذب فسفر به وسیله گندم و فعالیت آنزیم‌های فسفاتاز اسیدی و قلیایی در خاک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری خاکشناسی، پردیس علوم و تحقیقات خوزستان، دانشگاه آزاد اسلامی، اهواز، ایران گروه خاکشناسی، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران

2 گروه خاکشناسی، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران

3 استادیار موسسه تحقیقات خاک و آب ، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

4 دانشیار بخش تحقیقات خاک و آب مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان قزوین، سازمان تحقیقات، آموزش و ترویج کشاورزی، قزوین، ایران

چکیده

کمبود فسفر و نیز کارایی جذب پایین این عنصر در خاک‌های آهکی یکی از مشکلات مهم در سامانه‌های زراعی مناطق خشک و نیمه‌خشک می‌باشد. به‌منظور بررسی میزان جذب فسفر و فعالیت آنزیم‌های فسفاتاز اسیدی و قلیایی در سه نوع خاک با مقادیر متفاوت فسفر قابل‌جذب (Qazvin1، Qazvin2 و (Dizanو با کاربرد 50 میلی‌گرم فسفر بر کیلوگرم خاک از منابع کودهای گوسفندی، کمپوست زباله ‌شهری و سوپرفسفات ‌تریپل در شرایط گلخانه و تحت کشت گندم، تحقیقی در سال 1394 به‌صورت آزمایش‌های فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار به مرحله اجرا درآمد. نتایج نشان داد که میزان فعالیت آنزیم فسفاتاز قلیایی بالاتر از اسیدی و در خاک Dizan نسبت به سایر خاک‌های موردبررسی با افزایش معنی-داری همراه شد. میزان فعالیت آنزیم فسفاتاز قلیایی بیش از 7/2 برابر فسفاتاز اسیدی در خاک Dizanبود. میزان تأثیر کمپوست زباله شهری بر فعالیت آنزیم فسفاتاز اسیدی نسبت به گوسفندی و سوپرفسفات‌تریپل به ترتیب 3/82 و 1/62 و بر فعالیت آنزیم فسفاتاز قلیایی به ترتیب 6/52 و 6/87 میکروگرم پارانیتروفنل بر گرم خاک خشک بر ساعت بیشتر بود. در برهم‌کنش خاک و کود، بیشترین فعالیت آنزیم فسفاتاز اسیدی از خاک Dizan در تیمار با کمپوست زباله شهری و سوپرفسفات تریپل به ‌دست آمد. در خاک Dizan با کاربرد سوپرفسفات‌ تریپل، غلظت جذب فسفر توسط گیاه نسبت به کود گوسفندی، 3/34 درصد، کود کمپوست زباله شهری، 9/20 درصد و نسبت به شاهد، 7/65 درصد افزایش داشت. در این خاک میزان فسفر باقی‌مانده خاک پس از برداشت گیاه در تیمار با کود سوپرفسفات تریپل نسبت به تیمار با کود گوسفندی، 3/33 درصد، کود کمپوست زباله شهری، 7/61 درصد و شاهد 4/ 72 درصد افزایش نشان داد. با توجه به نتایج حاصل از این تحقیق در خاک‌های آهکی با کاربرد کمپوست زباله شهری حداکثر فعالیت آنزیمی فسفاتاز حاصل می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of organic and chemical fertilizers on the uptake of phosphorus by wheat and activity of acidic and alkaline phosphatase enzymes in soil

نویسندگان [English]

  • jafar shahabifar 1
  • Ebrahim Panahpour 2
  • Farhad Moshiri 3
  • Ali Gholami 2
  • Mehrzad Mostashari 4
1 PhD student of Soil Science, Khouzestan Science and Research Branch, Islamic Azad University, Khouzestan, Iran
2 Department of Soil Science, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
3 Assistant Professor of Soil and Water Research Institute, Agricultural and Natural Resources Research Center, (AREEO), Karaj, Iran.
4 Associate Professor of Soil and Water Research Section, Qazvin Agricultural and Natural Resources Research and Education Center, Agricultural and Natural Resources Research Center, (AREEO), Qazvin, Iran.
چکیده [English]

Phosphorus deficiency is one of the most important problems in calcareous agricultural soils of arid and semi-arid regions. This study was conducted to investigate the phosphorus uptake and activity of acidic and alkaline phosphatase enzymes in three types of soils with different levels of available phosphorus (Qazvin1, Qazvin2 and Dizan) treated with 50 mg/kg phosphorus by using sheep manure, municipal solid waste compost and triple superphosphate under greenhouse conditions in 2015 as a completely randomized design with three replications. The results showed that the acidic and alkaline phosphatase activity in Dizan were significantly higher than other studied soils. The alkaline phosphatase activity was more 2.7 times than acidic phosphatase in the soil. The influence of municipal solid waste compost on acidic and alkaline phosphatase enzymes activity were 82.3 and 62.1 p- Nitro phenol (µg/g-1dwt h-1) and 52.6 and 87.6 p- Nitro phenol (µg/g-1dwt h-1) higher than sheep manure and triple superphosphate treatments respectively. The results of soil and fertilizers interaction showed that, acidic phosphatase enzyme activity in municipal solid waste compost and triple superphosphate were more than sheep manure in Dizan soil sample. The phosphorus uptake by plant was 34.3, 20.9 and 65.7 % in Dizan treated soil sample with sheep manure, municipal solid waste and control respectively. In Dizan soil residual phosphorus after harvest of wheat in triple superphosphate treatment was 33.3, 61.7 and 72.4 % compared to sheep manure, municipal solid waste compost and control, respectively. According to the results the maximum phosphatase enzyme activity was obtained in treated calcareous soil with municipal waste compost.

کلیدواژه‌ها [English]

  • alkaline phosphatase
  • compost
  • sheep manure
  • triple super phosphate
  • wheat
Ahmadpoor- Sefidkoohi A., Ghajar-Sepanlu M., and Bahmaiyar M.A. 2013. Effects of several consecutive period of organic and chemical fertilizers on nitrogen, phosphorus and potassium, and some growth characteristics of wheat. Journal of Agricultural Science and Sustainable Production, 22 (4):72-86. (In Persian)
Ali Ahmad Korori S., Khoshnevis M., Shirvany A., and Matinizadeh M. 2011. Pollution effects of the Persian Gulf War on the southern regions of Iran. Jahad Publication of Tarbait Moalem, 380 p.
Baldoni G. 1996. The influence of compost and sewage sludge on agriculture crops in: De Bertoldi et al. (Ed.). The Science of Composting. Blackie Press, London. pp. 430-438.
Baure A., and Black A.L. 1992. Organic carbon effects on available water. Soil Science Amerhca Journal, 56: 248-254.
Bhattacharyya P., Chakrabarti K., and Chakraborty A. 2005. Microbial biomass and enzyme activities in submerged rice soil amended with municipal solid waste compost and decomposed cow manure. Chemosphere.60: 310-318. Biochemistry, 39: 1294-1302.
Blagodatsky S.A., and Richter O. 1998. Microbial growth in soil and nitrogen turnover: A theoretical model considering the activity state of microorganisms. Soil Biology and Biochemistry, 30: 1743-1755.
Cong P.T., and Merckx R. 2005. Improving phosphorus availability in two upland soils of Vietnam using Tithonia diversifolia H. Plant and Soil, 269: 11-23.
Dick W.A., and Tabatabai, M.A. 1993. Significance and potential uses of soil enzymes. In: Metting, F.B. (Ed.), Soil Microbial Ecology: Application in Agricultural and Environmental Management. Marcel Dekker, New York: 95- 125.
Eivazi F., and Tabatabai M.A. 1977. Phosphatases in soils. Soil Biology and Biochemistry, 9: 167-172.
Fereidooni-Naghani M., Raeisi F., and Fallah S. 2009. The effect of nitrogen source and amount on the calcareous soil enzyme activity under cultivation of maize. Journal of Water and Soil. 23(4): 127-136. (In Persian)
Forghani A. 2003. Study on biochemical and fulvic and humic acid characteristics of different soils amended with various organic matters. Eighth Soil Science Congress of Iran, Rasht. 12-9- September, Page 15. (In Persian)
Garg S., and Bahl G.S. 2008. Phosphorus availability to maize as influenced by organic manures and fertilizer P associated phosphatase activity in soils. Bioresource Technology. 99 (13): 5261-5996.
Guimaraes L.H.S., Simone C.P.N., and Michele M. 2006. Screening of filamentous fungi for production of enzymes of biotechnological interest. Brazilian Journal of Microbiolog, 37: 474-480.
Guo F., Yost R.S., Hue N., Evensen C.I., and Silva J.A. 2000. Changes in phosphorus fractions in soils under intensive plant growth. Soil Science Society of America, 64: 1681-1689.
Hallajnia A., Haghnia Gh., Photovva A., and Khorasani R. 2006. The impact of organic matter on soil P availability in calcareous soils. Scientific and Research Journal of Agriculture and Natural Resources Science and Technology,10, (4a): 121-132. (In Persian)
Harrison A. F. 1987. Soil Organic Phosphorus. C.A.B. International United Kingdom. 257p.
Hojjati S., Noorbakhsh F., Khavazi K. 2006. The effect of sewage sludge on microbial biomass index, soil enzyme activity and corn yield. Journal of Soil Research (Soil and Water Sciences), 25(1): 12-21. (In Persian)
Johansson E., Krantz-Rülcker C., Zhang B.X., and Öberg G. 2000. Chlorination and biodegradation of lignin. Soil Biology and Biochemistry, 32: 1029-1032.
Juma N.G., and Tabatabaei M.A. 1977. Effects of trace elements on phosphatase activity in soils. Soil Science Society of America Journal, 41: 343–346.
Khan A., Jilani G., Akhtar M.S., Saqlan Naqvi S.M., and Rasheed M. 2009. Phosphorus solubilizing bacteria: Occurrence, Mechanisms and their role in crop production. Journal of Agricultural and Biological Science, 1(11): 48-58.
Kiss S., Stefanic G., and Dragan-Bularda M. 1974. Soil enzymology in Romania (Part I). Contrib. Bot Cluj, pp. 207-219.
Kizilkaya R., Askin T., Bayarkli B., and Saglam M. 2004. Microbiological characteristics of soils contaminated with heavy metals. European Journal of Soil Biology, 40: 95-102.
Kuo S. 1996. Phosphorus. In: Sparks D.L. (Ed.), Methods of Soil Analysis-Part 3. Chemical Methods No. 5. Soil Science Society of America and American Society of Agronomy, Madison, pp. 869-919.
Leytem A.B., and Westermann D. T. 2005. Phosphorus availability to barley from manures and fertilizers on a calcareous Soil. Soil Science, 170: 401-408.
Liang Y., Yang Y., Yang Ch., Shen Q., Zhou J., and Yang L. 2003. Soil enzymatic activity and growth rice and barley as influenced by organic manure in an anthropogenic soil. Geoderma, 115: 149-160.
Mandal A., Patra A.K., Singh D., Swarup A., and Masto R.E. 2007. Effect of long-term application of manure and fertilizer on biological and biochemical activities in soil during crop development stage. Bioresource Technology, 98: 3585-3592.
Mirzashahi K., and Kiani Sh. 2008. Effects of sheep manure on amounts of chemical fertilizer consumption in corn. Journal of Investigative in Agricultural Science, 4(20): 174-186. (In Persian)
Mkhabela M. S., and Warman P.R. 2005. The influence of municipal solid waste compost on yield, soil phosphorus availability and uptake by two vegetable crops grown in a Pugwash sandy loam soil in Nova Scotia. Agriculture, Ecosystems and Environment, 106: 57-67.
Mohammadzadeh A.R., and Mivechi Langaroodi H. 1998. The method of manure and phosphorus fertilizers for decrease phosphorus fertilizers in Bushehr province soils. Journal of Soil and Water, 12(1): 20-27. (In Persian).
Nannipieri P. 1994. The potential use of soil enzymes as indicators of productivity, sustainability and pollution. In: Pankhurst in Sustainable Farming Systems. CSIRO, East Melbourne, pp. 238-244.
Olsen S.R., and Sommers L.E. 1982. Phosphorus. In A. L. page etal (Ed.) Methods of Soil analysis. Part 2. Agronomy 9:403-430. Am. Soc. Agro. Inc., Madison, WI.
Pettipas F.C. 2004. Soil and plant nutrient relationships in processing carrots. MSc. Thesis, Nova Scotia Agricultural College, Truro, Nova Scotia.
Philip A., Thomas J., and Xiaodun He. 2008. Bioavailability of Organically-Bound Soil Phophorous. College of Agricultural and Life Sciences, University of Wisconsin- Madison, USDA, National Research Initiative Competitive, Grant 94-37107-0356, and Hatch Project 3940.
Saha S., Mina B.L., Gopinath K.A., Kundu S., and Gupta H. S. 2008. Relative changes in phosphatase activities as influenced by source and application rate of organic composts in field crops. Bioresource Technology Journal, 99: 1750-1757.
Sastre I., Vicente M.A., and Lobo M.C. 1996. Influence of the application of sewage sludges on soil microbial activity. Bioresearch Technology, 57: 19-23.
Shahbazi K., and Davoodi M.H 2013. Evaluation of wheat phosphorous necessity with phosphorous absorption isotherm in calcareous soils. Journal of Soil and Water Science, 26: 1-17. (In Persian)
Smith A.N. 1996. The supply of soluble phosphorus to wheat plant from inorganic soil       phosphorus. Plant and Soil, 22: 314-316.
Tabatabai M.A., Weave R.W., Angle S., Bottomley P., Bezdicek D., Smith S., Tabatabai A., and Wollum A. 1994. Soil enzyme, In: Weaver RW et al., (Ed.) Methods in soil Analysis, Part 2: Microbiological and Biochemical properties, pp. 775-834.
Tabatabai M.A., and Bremner J.M. 1969. Use of p-nitrophenylphosphate for assay of soil phosphatase activity. Soil Biology Biochemistry, 1: 301-307.
Zahedifar M., Karimian N., Ronaghi A.M., Yasrebi J., and Emam Y. 2011. Phosphorus and zinc distribution in different parts and various growth stages of wheat under field conditions. Jornal of Water and Soil, 25(3): 436-445. (In Persian)