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اخبار و اعلانات

ارزیابی توان تولید سیدروفور توسط سیانوباکترهای جداسازی شده از اراضی شالیزاری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 هیات علمی گروه علوم و مهندسی خاک- دانشکده کشاورزی- دانشگاه شهرکرد

2 استاد گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز، ایران.

چکیده

سیدروفورها ترکیبات آلی با جرم مولکولی کم بوده که در شرایط کمبود آهن برای کمپلکس کردن آهن (III) به منظور تنظیم فعالیت­های مبتنی بر آهن بیان می­شوند. سیانوباکترها که ریزموجودات گرم منفی فتواتوتروف پروکاریوتی هستند، نوع هیدروکسامات سیدروفور در آنها غالب است. این بررسی با هدف قابلیت تولید سیدروفور سویه­های بومی سیانوباکترهای مزارع برنج در پژوهشکده زیست­فناوری طبرستان انجام گرفت. برای این منظور 30 جدایه از سیانوباکترهای شالیزارهای استان گیلان جداسازی شدند. توان تولید سیدروفور سویه­ها با استفاده از محیط کشت جامد و مایع حاوی کرم آزرول اس (CAS) ارزیابی شدند و آزمایش گلدانی برای ارزیابی کاربرد سویه­های برتر گزینش شده سیانوباکتری بر رشد و عملکرد گیاه برنج (رقم طارم هاشمی) انجام گردید. تیمار مایه­زنی سیانوباکتر با هفت سویه برتر گزینش شده و تیمار شاهد بدون مایه­زنی با سه تکرار در قالب طرح پایه بلوک کامل تصادفی در گلخانه انجام شد. نتایج حاصل از این پژوهش نشان داد که بیش‌ترین میزان نسبت هاله به کلونی در روش CAS- آگار جدایه­های Chroococcus sp. GGuCy-34 و Anabaena sp. GGuCy-49 به ترتیب 19/4 و 08/4 و در روش اسپکتروفتومتری سویه­های Anabaena sp. GGuCy-21 و Nostoc sp. GGuCy-47 به ترتیب 69/6 و 75/5 میکرومول بر لیتر در روز بود. در آزمایش گلدانی سویه Anabaena sp. GGuCy-42 بیش‌ترین عملکرد دانه (27/8 گرم بر گلدان)، سویه Chroococcus sp. GGuCy-34 بیش‌ترین جذب آهن (31/2 میلی­گرم در گلدان) و سویه Cylendrospermum sp. GGuCy-25 بیش‌ترین جذب نیتروژن (3/81 میلی­گرم در گلدان) و فسفر (7/11 میلی­گرم در گلدان) در برنج را نشان داد. نتایج حاصل از ارزیابی تولید سیدروفور سیانوباکترها در روش CAS- آگار با نتایج حاصل از آزمایش گلدانی مطابقت بیش‌تری داشت. افزایش جذب آهن را می­توان به علت تأثیر سیدروفور سویه­ها در فراهمی آهن برای گیاه دانست. باتوجه به اینکه سیانوباکترهای توانمند در تولید سیدروفور، می­توانند به عنوان عوامل محرک رشد گیاه هم مورد استفاده قرار گیرند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Siderophore Production Capacity by Isolated Cyanobacteria from Paddy Fields

نویسندگان [English]

  • saheb soodaee mashaee 1
  • Nasser Aliasgharzad 2

1 Faculty, Shahrekord University

2 Professor, Soil Science Department, Faculty of Agriculture, Tabriz University, Iran

چکیده [English]

Siderophores are low molecular weight organic chelators which mostly expressed under iron deficiency to chelate Fe (III) metal ions in order to regulate iron based activities. Cyanobacteria, which are Gram-negative photoautotrophic prokaryotic organisms, hydroxamate type of siderophore predominates. The objective of this investigation was to determine the potentials of some indigenous cyanobacteria for siderophore production in Tabarestan Biotechnology Institute in 2014. For this purpose, 30 strains of cyanobacteria were isolated from Guilan paddy field. Potentials of these strains for siderophore production were evaluated by chrome azorel-S assay (CAS-agar) through color change. Pot experiment was performed to evaluate the application of selected top strains of cyanobacteria on growth and yield of rice plant (cv. Tarom Hashemi). Cyanobacter inoculation treatment was selected with the top seven strains and control treatment without inoculation with three replications was performed in a randomized complete block design in the greenhouse. The results of this study showed that the highest rate of siderophore production in CAS- agar Chroococcus sp. GGuCy-34 and Anabaena sp. GGuCy-49, 4.19 and 4.08 respectively and in spectrophotometer method strains of Anabaena sp. GGuCy-21 and Nostoc sp. GGuCy-47, 6.69 and 5.75 (µmol/L.day) respectively. Anabaena sp. GGuCy-42 highest grain yield (8.27 g pot-1) Chroococcus sp. GGuCy-34 showed the highest iron uptake (2.31 mg pot-1) and Cylendrospermum sp. GGuCy-25 the highest N (81.3 mg pot-1) and P (11.7 mg pot-1) uptake in pot experiment in rice plant. The results of the evaluation of siderophore production of cyanobacteria in CAS- agar with the results of the pot experiment were more consistent. Increased iron uptake can be attributed to the effect of siderophore strains on plant iron availability. Due to the fact that cyanobacteria capable of producing siderophore can also be used as stimulants of plant growth.

کلیدواژه‌ها [English]

  • Chrome azurol S
  • Cyanobacteria
  • Spectrophotometer method
  • Siderophore-iron chelate
  • Rice
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تحقیقات کاربردی خاک
دوره 9، شماره 3
آذر 1400
صفحه 123-133
فایل ها
سابقه مقاله
  • تاریخ دریافت: 23 آذر 1399
  • تاریخ بازنگری: 21 تیر 1400
  • تاریخ پذیرش: 09 مرداد 1400
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