نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم خاک، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

2 علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

3 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

چکیده

استفاده از یک روش ارزان قیمت و دوستدار محیط زیست جهت کاهش خطرات زیست محیطی فلزات سنگین امری ضروری است. در این پژوهش تاثیر اسیدهای هیومیک و فولویک بر فراهمی مس و کادمیوم و جذب آن­ها توسط کرچک زینتی بررسی شد. تیمارهای آزمایشی شامل دو سطح 5/0 و 1 درصد (وزنی) اسیدهای هیومیک و فولویک به همراه تیمار شاهد در پنج تکرار به یک خاک آلوده به مس و کادمیوم افزوده شدند. پس از گذشت 90 روز، برخی ویژگی­های رویشی گیاه، غلظت مس و کادمیوم قابل دسترس خاک و غلظت  مس و کادمیوم ریشه و شاخساره اندازه­گیری شد. نتایج نشان داد که کاربرد اسید هیومیک در سطح 5/0 درصد باعث افزایش خصوصیات رویشی کرچک زینتی شد. در مقابل، افزودن سطح 1 درصد اسید هیومیک و سطح 5/0 و 1 درصد اسید فولویک ویژگی­های رویشی گیاه کرچک را به طور معنی­داری کاهش داد. کاربرد سطوح 5/0 و 1 درصد اسید هیومیک به­ترتیب سبب افزایش 19 و 37 درصدی غلظت مس قابل دسترس خاک و افزایش 30 و 44 درصدی کادمیوم قابل دسترس خاک شد. در تیمارهای 5/0 و 1 درصد اسید فولویک، به­ترتیب غلظت مس قابل دسترس خاک 35 و 39 درصد و کادمیوم قابل دسترس خاک 42 و 54 درصد بیش­تر از تیمار شاهد بود. کاربرد اسید هیومیک و اسید فولویک سبب افزایش معنی­دار غلظت مس و کادمیوم در شاخساره و ریشه کرچک زینتی در مقایسه با تیمار شاهد شد.  فاکتور انباشت زیستی و فاکتور انتقال در تمامی تیمارها کمتر از 1 به دست آمد و تنها کاربرد سطح 1 درصد اسید هیومیک و اسید فولویک سبب افزایش این دو فاکتور گردید. بر اساس نتایج فاکتور تجمع زیستی کرچک زینتی به عنوان یک گیاه انباشتگر محسوب نمی­شود اما کاربرد اسید هیومیک و فولویک توانست کارایی کرچک زینتی در پالایش فلزات مس و کادمیوم از خاک را افزایش دهد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Humic and Fulvic Acids on Phytoremediation Ability of Copper and Cadmium by Ornamental Castor Bean

نویسندگان [English]

  • Mojgan Jokar 1
  • Majid Hejaz 2
  • Mehdi Sarcheshmehpoor 1
  • Homayoon Farahmand 3

1 Department of Soil Science, Faculty of Agriculture, Shahid Bahonar univeraity of Kerman

2 Department of Soil Science, Faculty of Agriculture, Shahid Bahonar University Of Kerman

3 Department of Horticultural Science, Faculty of Agriculture, Shahid Bahonar Univerity of Kerman

چکیده [English]

It is essential to use of an inexpensive and eco-friendly method to reduce the environmental hazards of heavy metals. In this study, the effect of humic acid (HA) and fulvic acids (FA) on the availability of copper and cadmium and their uptake by ornamental castor oil was investigated in the greenhouse of Shahid Bahonar University of Kerman. Treatments including two levels of HA and FA (0.5 and 1 %) were added to a Cu-Cd polluted soil with five replications. A treatment received no organic acid as a control. After 90 days, some plant growth characteristics, available Cu and Cd and shoot, and root Cu, Cd concentration were measured. The results showed that application of 0.5% of humic acid to soil increased the growth characteristics of ornamental castor. In contrast, the addition of humic acid at the rate of 1% and fulvic acid at the rate of 0.5 and 1% significantly reduced plant growth characteristics. Soil application of 0.5 and 1% of humic acid increased available Cu by 19 and 37% and available Cd by 30 and 44%, respectively. Available Cu and Cd in soils treated with 0.5 and 1% of fulvic acid were 35 and 39%, and 42 and 54% higher than the control. Shoot and root Cu and Cd concentration were significantly increased in plants treated with 0.5 and 1% of humic and fulvic acids. Bioaccumulation and translocation factors were less than 1 in all treatments and increased in soil treated with 1 % of HA and FA. Based on these results, ornamental castor been could not be considered as a hyperaccumulator plants. However, humic and fulvic acids can increase the phytoremediation ability of ornamental castor for Cu and Cd removal.
 

کلیدواژه‌ها [English]

  • phytoremediation
  • Humic Substances
  • Bioaccumulation factor
  • Heavy metals
Auld D.L., Zanotto M.D., McKeon T., and John Morris B. 2009. Oil Crops. Springer Dordrecht Heidelberg, Germany, 548p.
Bandiera M., Mosca G., and Vamerali T. 2009. Humic acids affect root characteristics of fodder radish (Raphanus sativus L. var. oleiformis Pers.) in metal – polluted wastes. Desalination, 247: 78 – 91.
Chen Y. 1996. Organic Matter Reactions Involving Micronutrients in Soils and Their Effect on Plants. In: Piccolo, A. (Ed), Humic Substances in Terrestrial Ecosystems. Elsevier, Amsterdam. pp. 507–530.
Chen Y.X., Lin Q., Luo Y.M., He Y.F., Zhen S.J., Yu Y.L., Tian G.M., and Wong M.H. 2003. The role of citric acid on the phytoremediation of heavy metal contaminated soil. Chemosphere, 50: 807-811.
Dehno A.H., and Mohtadi A. 2018. The effect of different iron concentrations on lead accumulation in hydroponically grown Matthiola flavida Boiss. Ecological Research, 33: 757-765.
Duan D., Tong J., Xu Q., Dai L., Ye J., Wu H., Xu C., and Shi, J. 2020. Regulation mechanisms of humic acid on Pb stress in tea plant (Camellia sinensis L.). Environmental Pollution, 267: p.115546.
Evangelou M.W.H., Ebel M., and Schaeffer, A. 2006. Evaluation of the effect of small organic acids on phytoextraction of Cu and Pb from soil with tobacoo nicotiana tabacum. Chemosphere, 63: 996-1004.
Evangelou M.W.H., Daghan H., and Schaeffer A. 2004. The influence of humic acids on the phytoextraction of cadmium from soil. Chemosphere, 57:207-213.
Gan L., Yan Z., Ma Y., Zhu Y., Li X., Xu J., and Zhang, W. 2019. pH dependence of the binding interactions between humic acids and bisphenol A - A thermodynamic perspective. Environmental Pollution, 225: 113292.
Gee, G.W., and Bauder, J.W., 1986. Particle size analysis. In: Klute, A. (Ed.), Methods of Soil Analysis: Physical and Mineralogical Methods, Part 1, Second ed. Soil Science Society of America Inc., Madison, WI, pp. 383–409.
Ghosh M., and Singh S.P. 2005. A review on phytoremediation of heavy metal and utilization of its by-products. Applied ecology and environmental research, 3: 1-18.
Gitipour S., Akbarpour F.,Baghdadi M. and Mehrdadi N. 2022. Influence of the organic acids on the heavy metals mobility and distribution in the contaminated soils. Applied Soil Research, 9(4): 62-73.
Guo Y. and Marschner H. 1995. Uptake, distribution, and binding of cadmium and nickel in different plant species. Journal of Plant Nutrition, 18: 2691-2706.
Haberhauer G., Rafferty B., Strebl F., and Gerzabek, M.H. 1998. Comparison of the composition of forest soil litter derived from three different sites at various decompositional stages using FTIR spectroscopy. Geoderma, 83: 331-342.
Haghighi M.,  and Kafi M. 2010. Effect of humic acid on the accumulation of cadmium, nitrate and changes of nitrate reductase activity in lettuce.  Journal of Horticulture Science (Agricultural Sciences and Technology), 1: 53-58. (In Persian)
Hamzenejad taghlidabad R. and Khodaverdiloo H. 2020. Quantitative assessment of soil heavy metals pollution. Applied Soil Research, 8(2): 37-52.
Jones, J.B., 2001. Laboratory Guide for Conducting Soils Tests and Plant Analysis. CRC Press, New York.
Kadem D., Rached O., Krika A., and Gheribi-Aoulmi, Z. 2004. Statistical analysis of vegetation inci­dence on contamination of soils by heavy metals (Pb, Ni and Zn) in the vicinity of an Iron steel industrial plant in Algeria. Environmetrics, 15: 447–462.
Kansara K., Paruthi A., Misra S.K., Karakoti A.S., and Kumar, A. 2019. Montmorillonite clay and humic acid modulate the behavior of copper oxide nanoparticles in aqueous environment and induces developmental defects in zebra fish embryo. Environmental Pollution, p. 113313.
Karimi A., Khodaverdiloo, H., and Rasouli-Sadaghiani M.H. 2018. Plant tolerance, accumulation and remediation of Pb by three rangeland plant species in a calcareous soil in West Azerbaijan Province. Journal of Natural Environment, 70: 907-922. (In Persian)
Khodaverdiloo H., Han F.X., Hamzenejad Taghlidabad R., Karimi A., Moradi N., and Kazery J. A. 2020. Potentially toxic element contamination of arid and semi-arid soils and its phytoremediation. Arid Land Research and Management, 34: 361-91.
Kim H.C., Yu M.J., and Han I. 2006. Multi-method study of the characteristic chemical nature
of aquatic humic substances isolated from the Han River, Korea. Applied Geochemistry, 21:
1226–1239.
Komar L., Tu C., Zhang W., Cai Y., and Kennelley E.K. 2001. A fern that hyperaccumulates arsenic. Nature Journal, 409: 579-585.
Kulikowska D., Gusiatin Z.M., Bułkowska K., and Klik B. 2015.  Feasibility of using humic substances from compost to remove heavy metals (Cd, Cu, Ni, Pb, Zn) from contaminated soil aged for different periods of time. Journal of Hazardous Materials, 300: 882–891.
Lasat M.M. 2002. Phytoextraction of heavy metals: A review of biological mechanisms. Journal of Environmental Quality, 31: 109–120.
Li X., Peng P., Long J., Dong X., Jiang K., and Hou, H. 2020. Plant-induced insoluble Cd mobilization and Cd redistribution among different rice cultivars. Journal of Clean Production, 256: p. 120494.
Lindsay W.L., and Norvell W.A. 1978. Development of DTPA soil test for Zinc, Iron, manganese and copper. Soil Science Society of American Journal, 42:421–428.
Page, A.L., Miller R.H., and Keeney, D.R. 1982. Methods of Soil Analysis, Part 2, Chemical and Microbiological Properties. American Society of Agronomy, Inc. Soil Science of America, Inc. Madison, Wisconsin, USA, 1195 p.
Park S., Kim K.S., Kang D., Yoon H., and Sung K. 2013. Effects of humic acid on heavy metal uptake by herbaceous plants in soils simultaneously contaminated by petroleum hydrocarbons. Environmental Earth Sciences, 68: 2375-2384.
Rashid I., Murtaza G., Ahmed Dar A., and Wang Z. 2020. The influence of humic and fulvic acids on Cd bioavailability to wheat cultivars grown on sewage irrigated Cd-contaminated soils. Ecotoxicology and Environmental Safety, 205: 111347.
Rasouli-Sadaghiani M.H., Karimi H., Khodavediloo H., Moradi M., and Barin, N. The Role of Humic Acid on Phytoremediation of Pb through a Pasture Collar Plant (Xeanthium vetelus). Water and Soil Science, 27: 249-266 (In Persian)
Rauthan B.S., and Schnizer, M. 1981. Effects of soil fulvic acid on the growth and nutrient content of cucumber plant. Plant and soil, 63: 491-495.
Robinson B.H., Millis T.M., Petit D., Fung L.E., Green S.R, and Clothier B.E. 2000. Natural and induced cadmium accumulation poplar and willow: implications for phytoremediation. Plant Soil, 227: 301–306.
Rodríguez F.J., Schlenger P., and García-Valverde M. 2016. Monitoring changes in the structure and properties of humic substances following ozonation using UV–Vis, FTIR and 1H NMR techniques. Science of the Total Environment, 541: 623-637.
Rong Q., Zhong K., Huang H., Li C., Zhang C., and Nong X. 2020. Humic acid reduces the available cadmium, copper, lead, and zinc in soil and their uptake by tobacco. Applied Science, 10: p.1077.
Rothery E. 1988. Analytical methods for graphite tube atomizers. Varian Australia Pty Ltd, Mulgrave, p.193.
Salm M.A., Morton D.W., Johnson B.B., and Angove M.J. 2020. Adsorption of humic and fulvic acids onto a range of adsorbents in aqueous systems, and their effect on the adsorption of other species: A review, Separation and Purification Technology, p. 116949.
Schützendübel A., and Polle, A. 2002. Plant responses to abiotic stresses: Heavy metal‐induced oxida­tive stress and protection by mycorrhization. Journal of Experimental Botany, 53:1351–1365.
Senden M.H.M.N., Van Paassen F.J.M., VanDerMeer A.J.G.M., and Wolterbeek H.T.H. 1990. Cadmium–citric acid–xylem cell wall interactions in tomato plants. Plant, Cell and Environment, 15: 71–79.
Senesi N., D’Orazio V., and Ricca G. 2003. Humic acids in the first generation of Eurosoils. Geoderma, 116: 325–344.
Sun B., Zhao F. G., Lombi E., and Mc Grath, S.P. 2001. Leaching of heavy metals from contaminated soil using EDTA. Enviromental Pollution, 113: 111-120.
Ullah A., Heng S., Munis M.F.H., Fahad S., and Yang X. 2015. Phytoremediation of heavy metals assisted by plant growth promoting (PGP) bacteria: a review. Environmental and Experimental Botany, 117: 28-40.
Yu G., Jiang X., He W., and He Z. 2002. Effect of humic acids on species and activity of cadmium and lead in red soil. Acta Scientiae Circumstantiae, 22: 508-513.
Walkley, A., and Black. 1934. An examination of the dehligaroff method for determining organic matter and a proposed modification of the chromic acid titration method. Soil Science, 37: 29-38.
Wang Q., Li Zh., Cheng Sh., and Wu, Zh. 2010. Effects of humic acids on phytoextraction of Cu and Cd from sediment by Elodea nuttallii. Chemospher, 78: 604 – 608.
Zhang Y., Yang X., Zhang S., Tian Y., Guo W., and Wang J. 2013. The influence of humic acids on the accumulation of lead (Pb) and cadmium (Cd) in tobacco leaves grown in different soils. Journal of Soil Science and Plant Nutrition, 13: 43-53.