پیامد مایه‌زنی قارچ اندوفیت Piriformospora indica بر گیاه‌پالایی سرب توسط گونه چمنی فستوکای پابلند (Festuca arundinacea) رقم Tomahawk

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه

2 استادیار گروه علوم باغبانی دانشگاه ارومیه

3 استادیار گروه علوم خاک، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه.

4 استادیار گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه.

5 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه.

چکیده

مایه­زنی میکروبی، در بهبود رشد و افزایش تحمل گیاهان به تنش­های محیطی و پالایش سبز خاک­های آلوده به فلزات سنگین، مؤثرند. این مطالعه به‌منظور بررسی تأثیر  مایه­زنی قارچ Piriformospora indica در گیاه­پالایی سرب توسط گونه چمنی فستوکای پابلند (Festuca arundinacea) رقم Tomahawk در سطوح مختلف آلودگی سرب، در آزمایشی گلدانی به‌صورت فاکتوریل در قالب طرح پایه بلوک­های کامل تصادفی و در سه تکرار اجرا شد که عامل‌های آزمایشی شامل مایه­زنی قارچ در دو سطح (مایه­زنی و عدم مایه­زنی با قارچindica  P.) و تنش آلودگی سرب در سه سطح (0، 500 و 800 میلی­گرم بر کیلوگرم خاک) بودند. سرب در هر دو سطح آلودگی 500 و 800 میلی­گرم بر کیلوگرم خاک به­ترتیب سبب کاهش 46/31 و 98/43 درصد وزن خشک اندام هوایی، 2/31 و 32/44 درصد عملکرد نسبی شاخساره، 89/10 و 74/15 درصد محتوای نسبی آب برگ، 8/47 و 55/47 درصد محتوای کلروفیل a، 41/47 و 22/50 درصد محتوای کلروفیل b، 22/5 و 64/11 درصد غلظت فسفر ریشه، 44/11 و 47/14 درصد غلظت نیتروژن برگ و افزایش 49/27 و 89/20 درصد محتوای پرولین و 13/6 و 71/14 درصد قند محلول برگ شد.  تیمار مایه­زنی با قارچ P. indica نسبت به تیمار بدون مایه­زنی در هر دو سطح آلودگی500 و 800 میلی­گرم بر کیلوگرم خاک سرب به­ترتیب با افزایش 34/26 و 67/18 درصدی انباشت سرب در ریشه و افزایش 13/64 و 83/59 درصد سرب قابل استخراج توسط ریشه سبب افزایش 22/40 و 12/18 درصد وزن تر ریشه، 22/51 و 59/50 درصد وزن خشک ریشه، 32/44 و 72/40 درصد محتوای کلروفیل a، 98/16 و 95/33 درصد محتوای کاروفیل b، 71/15 و 6/17 درصد غلظت فسفر ریشه، 21/16 و 92/13 درصد غلظت فسفر برگ شد. می­توان نتیجه­گیری کرد که مایه­زنی میکروبی با قارچ P. indica باعث افزایش انباشت سرب در ریشه­ی گیاه چمن فستوکا می­شود. بنابراین مایه­زنیقارچ P. indica می­تواند به‌عنوان یک تیمار زیستی مفید در بهبود کارایی گیاه­پالایی سرب توسط گیاه چمن فستوکا رقم Tomahawk در خاک­های آلوده استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Consequences of Endophytic Fungus Piriformospora Indica Inoculation on Phytoremediation of Lead by Tall Fescue (Festuca Arundinacea cv. Tomahawk)

نویسندگان [English]

  • mahdieh mirzaei mashhood 1
  • Javad Rezapour fard 2
  • mohsen barin 3
  • hadi alipour 4
  • zohreh Jabbarzadeh 5
1 Ph.D. Student, Department of Horticultural Sciene, Faculty of Agriculture, Urmia University, Urmia.
2 Assistant professor, Department of Horticulture science, Faculty of Agriculture, Urmia university
3 Assistant Professor, Department of Soil Sciene, Faculty of Agriculture, Urmia University, Urmia.
4 Assistant Professor, Department of Plant Production and Genetic, Faculty of Agriculture, Urmia University, Urmia.
5 Associate Professor, Department of Horticultural Sciene, Faculty of Agriculture, Urmia University, Urmia
چکیده [English]

Microbial inoculation is effective in improving plant growth and tolerance to environmental stresses, and green refinement of contaminated soils with heavy metals. This study was carried out in order to evaluate the effect of Piriformospora indica inoculation on phytoremediation of lead by tall fescue (Festuca arundinacea cv. Tomahawk) at different levels of Pb contamination. It was performed in pot experiment as factorial based on randomized complete block design with three replications which the experimental factors included inoculation, and non-inoculation with P. indica, and Pb contamination (0, 500, and 800 mg kg-1 of soil). Pb contamination at levels of 500 and 800 mg kg-1 of soil caused a decrease of 31.46% and 43.98% of shoot dry weight, 31.2% and 44.32% of relative yield, 10.89%, and 15.74% of leaf relative water content, 47.8%, and 47.55% of chlorophyll a, 47.41%, and 50.22% of chlorophyll b, 5.22%, and 11.64% of root phosphorus content, 11.44%, and 14.47% leaf nitrogen content, and increase of 27.49% and 20.89% of leaf proline and 6.13% and 14.71% of leaf soluble sugars respectively. P. indica inoculation in compared with non- inoculation in Pb contamination at both 500 and 800 mg kg-1 of soil by increasing the accumulation of 26.34% and 18.67% Pb in roots as well as increasing the 64.13% and 59.83% root metal extraction caused an increase of 40.22% and 18.12% of root fresh and 51.22% and 50.59% dry weight, 44.32% and 40.72% of chlorophyll a and 16.98% and 33.95% of chlorophyll b content, 15.71% and 17.6% of root phosphorus concentration and 16.21% and 13.92% of shoot phosphorus concentration, respectively. It is inferred that microbial inoculation with P. indica increases the accumulation of Pb in the roots of Festuca arundinacea and it is suggested that P. indica-inoculated Festuca arundinacea cv. Tomahawk can be used as biologically useful approach in phytoremediation of Pb-contaminated soils.

کلیدواژه‌ها [English]

  • Heavy metals
  • transfer factor
  • leaf relative water content
  • compatible solutions
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