تاثیر نیتروژن و نسبت نیترات به آمونیوم بر شاخص های رشد و عملکرد اسانس مرزنجوش بخارایی (Origanum vulgare ssp. Gracile) در شرایط شور

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه ارومیه

2 ارومیه، دانشگاه ارومیه

3 عضوهیات علمی دانشگاه ارومیه

چکیده

به منظور بررسی تأثیر سطوح متفاوت نیتروژن و نسبت های مختلف نیترات به آمونیوم در شرایط شور و غیرشور بر شاخص های رشد، درصد و عملکرد اسانس مرزنجوش بخارایی، آزمایشی به صورت فاکتوریل در قالب طرح بلوک های کامل تصادفی با ۳۶ تیمار و ۳ تکرار انجام گرفت. نیتروژن مورد استفاده در ۳ سطح (۵، ۱۰ و ۱۵ میلی مولار)، نسبت های مختلف نیترات به آمونیوم در۶ سطح (۱۰۰ :۰، ۸۷/۵: ۱۲/۵، ۷۵: ۲۵، ۵۰ : ۵۰، ۲۵ : ۷۵ و ۰ : ۱۰۰) و شوری در ۲ سطح (صفر و ۵۰ میلی مولار سدیم کلرید) به عنوان فاکتورهای آزمایش در نظر گرفته شد. نتایج نشان داد در شرایط عدم تنش شوری بیشترین مقدار وزن تر (۲/۴۲ گرم)، وزن خشک (۸/۱۶ گرم)، و عملکرد اسانس (۹/۱۵۸ میلی‌گرم در گلدان) در سطح نیتروژن ۱۵ میلی مولار و نسبت ۷۵ : ۲۵ نیترات به آمونیوم به دست آمد؛ در صورت اعمال تنش شوری هر سه صفت فوق در تمام تیمارها کاهش یافت، به طوری که تحت این شرایط حداکثر وزن تر (۳۴/۳۳ گرم)، وزن خشک (۵۶/۴ گرم)، و عملکرد اسانس (۳۵/۹۴ میلی‌گرم در گلدان) در تیمار ۱۵ میلی مولار نیتروژن و نسبت ۵۰ : ۵۰ نیترات به آمونیوم مشاهده گردید. اثرات اصلی هر سه تیمار تاثیر بسیار معنی داری بر درصد اسانس داشت؛ به طوری که از غلظت ۱۵ میلی مولار نیتروژن، نسبت ۷۵ : ۲۵ نیترات به آمونیوم و در شوری ۵۰ میلی مولار بیشترین درصد اسانس حاصل شد. می توان نتیجه گرفت غلظت ۱۵ میلی مولار نیتروژن و نسبت های ۷۵ : ۲۵ و ۵۰ : ۵۰ نیترات به آمونیوم به ترتیب در محیط غیر شور و شور از نظر تغذیه نیتروژنی جهت افزایش بیوماس تولیدی مرزنجوش مناسب می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of nitrogen and nitrate: ammonium ratio on growth indices and essential oil content of Origanum vulgare ssp. Gracile under saline conditions

نویسندگان [English]

  • sajad nematzade 2
  • Amir Rahimi 3
1
2 urmia university
3 urmia university
چکیده [English]

In order to evaluate effects of different levels of nitrogen and different ratios of nitrate: ammonium under saline and non-saline conditions on growth indices, essential oil content and yield of Origanum Vulgare ssp. Gracile, a factorial experiment was carried out in randomized complete block design (RCBD) with third factors including nitrogen levels (5, 10, and 15 mM), nitrate: ammonium ratios (100:0, 87.5:12.5, 75:25, 50:50, 25:75, 0:100), and salinity levels (0 and 50 mM NaCl) in three replications. The results showed that under non-saline condition, the highest fresh weight (33.34 g), dry weight (8.16 g), and essential oil yield (158.9 mg/pot) were obtained by 15 mM nitrogen and 75:25 nitrate: ammonium ratio. Under saline condition, all the three above-mentioned traits were reduced in all treatments however under this condition, the highest fresh weight (33.34 g), dry weight (4.56 g) and essential oil yield (94.35 mg/pot) were observed by 15 mM nitrogen and 50:50 nitrate: ammonium ratio. The main effects of all treatments on essential oil content were significant (p ≤ 0.01); so that the highest essential oil content obtained by 15 mM nitrogen, 75:25 nitrate: ammonium ratio, and 50 mM salinity. In conclusion, 15 mM nitrogen with 75:25 and 50:50 nitrate: ammonium are recommended for non-saline and saline conditions, respectively.

کلیدواژه‌ها [English]

  • "ammonium
  • " Nitrate
  • " Nitrogen
  • " Origanum
  • " Salinity"
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