نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه تربیت مدرس

2 (استاد دانشگاه ترتبیت مدرس ) فرسایش و رسوب

3 گروه معدن، دانشگاه تربیت مدرس

چکیده

سیانوباکتری‌ها یکی از اجزای اصلی پوسته‌های زیستی خاک در مناطق خشک و نیمه‌خشک هستند که در لایه سطحی خاک رشد می‌کنند و با ترشح مواد پلی‌ساکاریدی در چرخه عناصر شرکت کرده و نقش مهمی در بهبود کیفیت و حاصلخیزی خاک دارند. بر همین اساس، در سال‌های اخیر با کشت و تولید زی‌توده موردنیاز در شرایط آزمایشگاهی و با تلقیح در سطح خاک در مباحث حفاظت منابع خاک و آب مورداستفاده قرارگرفته‌اند. ولی تاکنون ارزیابی جامعی برای شناسایی محیط‌های کشت مناسب ریزموجودات خاک‌زی گزارش نشده است. در همین راستا، پژوهش حاضر باهدف انتخاب محیط کشت بهینه برای رشد سیانوباکتری‌ها در شرایط آزمایشگاهی انجام شد. برای این هدف به مقایسه عملکرد سه محیط کشت BBM، BG11 و CHU10 در مدت زمان رشد یک‌ماهه سیانوباکتری‌های خاک‌زی پرداخته شد. نتایج نشان داد سیانوباکتری‌های خاک‌زی در محیط کشت BBM نسبت به دو محیط کشت دیگر رشد بهتری داشته‌اند، به‌ طوریکه در انتهای دوره رشد تعداد سیانوباکتری‌های رشد یافته در محیط کشت‌های BBM، BG11 و CHU10 به ترتیب 91790، 48638 و 1491 عدد در یک میلی‌لیتر بوده است. بنابراین محیط کشت BBM به‌ عنوان محیط کشت بهینه برای استفاده در خصوص تهیه زی‌توده مورد نیاز از سیانوباکتری‌ها برای تلقیح در سطح خاک انتخاب و پیشنهاد می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of Different Culture Media on the Growth of Soil Cyanobacteria for Soil and Water Conservation

نویسندگان [English]

  • Atefeh Jafarpoor 1
  • Seyed Hamidreza Sadeghi 2
  • Mehdi Homaee 3
  • Behrouz Zarei Darki 1

1 Tarbiat Modares University

2 Professor

3 Tarbiat Modares University

چکیده [English]

Cyanobacteria are one of the main components of soil biocrusts in arid and semi-arid regions that grow in the surface layer of the soil. They actively participate in the cycle of elements by secreting polysaccharides and play an important role in improving soil quality and fertility. Accordingly, they have been used in soil and water resources conservation issues in recent years through cultivating and producing the required biomass in laboratory conditions and inoculating at the soil surface. However, the proper media for optimal growth of the soil microorganisms have not been reported comprehensively. The present study was conducted to select the optimal culture medium for the growth of cyanobacteria in experimental conditions. For this purpose, the performance of three culture media viz. BBM, BG11 and CHU10 was compared during the one-month growth period of soil cyanobacteria. In this regard, the types of grown microorganisms as well as the number of cyanobacteria present during the growth period of one-month and on average once every 4 days were examined. The results showed that soil cyanobacteria in BBM culture medium had better growth than the other two culture media and at the end of the growth period, the number of cyanobacteria grown in BBM, BG11 and CHU10 medium was 91790, 48638 and 1491, respectively, per milliliter. Therefore, BBM culture medium is proposed as the optimal culture medium for use in preparing the biomass required by cyanobacteria for inoculation at the soil surface.

کلیدواژه‌ها [English]

  • Biologic control of soil erosion
  • Growth elements
  • Soil microorganisms
  • Soil organic amendment
  • Soil protection
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